A cellular uptake and cytotoxicity properties study of gallic acid-loaded mesoporous silica nanoparticles on Caco-2 cells
Source abstract
Abstract
In this study, the effects of intracellular delivery of various concentrations of gallic acid (GA) as a semistable antioxidant, gallic acid-loaded meso- porous silica nanoparticles (MSNs-GA), and cellular uptake of nanoparticles into Caco-2 cells were inves- tigated. MSNs were synthesized and loaded with GA, then characterized using transmission electron micros- copy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, N2 adsorp- tion isotherms, X-ray diffraction, and thermal gravi- metric analysis. The cytotoxicity of MSNs and MSNs- GA at low and high concentrations were studied by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) test and flow cytometry. MSNs did not show significant toxicity in various concentrations (0-500 lg/ml) on Caco-2 cells. For MSNs-GA, cell viability was reduced as a function of incubation time and different concentrations of nano- particles. The in vitro GA release from MSNs-GA exhibited the same antitumor properties as free GA on Caco-2 cells. Flow cytometry results confirmed those obtained using MTT assay. TEM and fluorescent microscopy confirmed the internalization of MSNs by Caco-2 cells through nonspecific cellular uptake. MSNs can easily internalize into Caco-2 cells without deleterious effects on cell viability. The cell viability of Caco-2 cells was affected during MSNs-GA uptake. MSNs could be designed as suitable nanocarriers for antioxidants delivery.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Nanoparticle-Based Drug Delivery · Tannin, Tannase and Anticancer Activities
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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