Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
Source abstract
Abstract
Carbon-encapsulated iron nanoparticles (CEINs) are emerging as promising biomedical tools due to their unique physicochemical properties. In this study, the cytotoxic effect of CEINs (the mean diameter distribution ranges 46-56 nm) has been explored by MTT, LDH leakage, Calcein-AM/propidium iodide (PI) and Annexin V-FITC/PI assays in human melanoma (HTB-140), mouse melanoma (B16-F10) cells, and human dermal fibroblasts (HDFs). The results demonstrated that CEINs produce mitochondrial and cell membrane cytotoxicities in a dose (0.0001-100 μg/ml)-dependent manner. Moreover, the studies elucidated some differences in cytotoxic effects between CEINs used as raw and purified materials composing of the carbon surface with acidic groups. Experiments showed that HTB-140 cells are more sensitive to prone early apoptotic events due to raw CEINs as compared to B16-F10 or HDF cells, respectively. Taken together, these results suggest that the amount of CEINs administered to cells and the composition of CEINs containing different amounts of iron as well as the carbon surface modification type is critical determinant of cytotoxic responses in both normal and cancer (melanoma) cells.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Graphene and Nanomaterials Applications · Nanoparticles: synthesis and applications · Carbon Nanotubes in Composites
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (69)
Show source-provided references
1. Ahmed N, Fessi H, Elaissari A (2012) Theranostic applications of nanoparticles in cancer. Drug Discov Today 17(17–18):928–93410.1016/j.drudis.2012.03.010 ↗
2. Akhavan O, Ghaderi E, Akhavan A (2012) Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. Biomaterials 33(32):8017–802510.1016/j.biomaterials.2012.07.040 ↗
3. Berman SMC, Walczak P, Bulte JWM (2011) Tracking stem cells using magnetic nanoparticles. Nanomed Nanobiotechnol 3:343–35510.1002/wnan.140 ↗
4. Boehm HP (1994) Some aspects of the surface chemistry of carbon blacks and other carbons. Carbon 32:759–76910.1016/0008-6223(94)90031-0 ↗
5. Borysiuk J, Grabias A, Szczytko J, Bystrzejewski M, Twardowski A, Lange H (2008) Structure and magnetic properties of carbon encapsulated Fe nanoparticles obtained by arc plasma and combustion synthesis. Carbon 46:1693–170110.1016/j.carbon.2008.07.011 ↗
6. Briley-Saebo K, Bjørnerud A, Grant D, Ahlstrom H, Berg T, Kindberg GM (2004) Hepatic cellular distribution and degradation of iron oxide nanoparticles following single intravenous injection in rats: implications for magnetic resonance imaging. Cell Tissue Res 316:315–32310.1007/s00441-004-0884-8 ↗
7. Bystrzejewski M, Huczko A, Lange H (2005) Arc plasma route to carbon-encapsuled magnetic nanoparticles for biomedical applications. Sens Actuators B-Chem 109:81–8510.1016/j.snb.2005.03.029 ↗
8. Bystrzejewski M, Cudziło S, Huczko A, Lange H, Soucy G, Cota-Sanchez G, Kaszuwara W (2007) Carbon encapsulated magnetic nanoparticles for biomedical applications: thermal stability studies. Biomol Eng 24:555–55810.1016/j.bioeng.2007.08.006 ↗
9. Bystrzejewski M, Klingeler R, Gemming T, Büchner B, Rümmeli MH (2011) Synthesis of carbon-encapsulated iron nanoparticles by pyrolysis of iron citrate and poly(vinyl alcohol): a critical evaluation of yield and selectivity. Nanotechnology 22(31):31560610.1088/0957-4484/22/31/315606 ↗
10. Chen L, Mccrate JM, Lee JC, Li H (2011) The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells. Nanotechnology 22(10):10570810.1088/0957-4484/22/10/105708 ↗
11. Chiappetta DA, Facorro G, de Celis ER, Sosnik A (2011) Synergistic encapsulation of the anti-HIV agent efavirenz within mixed poloxamine/poloxamer polymeric micelles. Nanomedicine 7:624–63710.1016/j.nano.2011.01.017 ↗
12. Clement JH, Schwalbe M, Buske N, Wagner K, Schnabelrauch M, Gornert P, Kliche KO, Pachmann K, Weitschies W, Hoffken K (2006) Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells. J Cancer Res Clin Oncol 132:287–29210.1007/s00432-006-0076-x ↗
13. Dam DH, Lee JH, Sisco PN, Co DT, Zhang M, Wasielewski MR, Odom TW (2012) Direct observation of nanoparticle-cancer cell nucleus interactions. ACS Nano 6:3318–332610.1021/nn300296p ↗
14. Davis MA, Zuckerman JE, Choi H, Seligson D, Tolcher A, Alabi CA, Yen Y, Heidel JD, Ribas A (2010) Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles. Nature 464(7291):1067–107010.1038/nature08956 ↗
15. Erdmann F, Lortet-Tieulent J, Schüz J, Zeeb H, Greinert R, Breitbart EW, Bray F (2013) International trends in the incidence of malignant melanoma 1953–2008 are recent generations at higher or lower risk? Int J Cancer 132(2):385–40010.1002/ijc.27616 ↗
16. Evans MS, Madhunapantula SV, Robertson GP, Drabick JJ (2013) Current and future trials of targeted therapies in cutaneous melanoma. Adv Exp Med Biol 779:223–25510.1007/978-1-4614-6176-0_10 ↗
17. Fisichella M, Dabboue H, Bhattacharyya S, Lelong G, Saboungi ML, Warmont F, Midoux P, Pichon C, Guérin M, Hevor T, Salvetat JP (2010) Uptake of functionalized mesoporous silica nanoparticles by human cancer cells. J Nanosci Nanotechnol 10:2314–232410.1166/jnn.2010.1917 ↗
18. Forsea AM, Del Marmol V, de Vries E, Bailey EE, Geller AC (2012) Melanoma incidence and mortality in Europe: new estimates, persistent disparities. Br J Dermatol 167(5):1124–113010.1111/j.1365-2133.2012.11125.x ↗
19. Gillich T, Benetti EM, Rakhmatullina E, Konradi R, Li W, Zhang A, Schlüter AD, Textor M (2011) Self-assembly of focal point oligo-catechol ethylene glycol dendrons on titanium oxide surfaces: adsorption kinetics, surface characterization, and nonfouling properties. J Am Chem Soc 133:10940–1095010.1021/ja202760x ↗
20. Grass RN, Athanassiou EK, Stark WJ (2007) Covalently functionalized cobalt nanoparticles as a platform for magnetic separations in organic synthesis. Angew Chem Int Ed Engl 46(26):4909–491210.1002/anie.200700613 ↗
21. Gupta AK, Gupta M (2005) Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles. Biomaterials 26:1565–157310.1016/j.biomaterials.2004.05.022 ↗
22. Hong SH, Lee JH, Lee J, Kim HY, Park JY, Cho J, Lee J, Han DW (2011) Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups. Int J Nanomedicine 6:3219–3231
23. Hosseinkhani H (2006) DNA nanoparticles for gene delivery to cells and tissue. Int J Nanotechnol 3(4):416–46110.1504/IJNT.2006.011172 ↗
24. Hosseinkhani H, Hong PD, Shyong YR, Ickowicz D, Farber IY, Domb AJ (2012) Development of 3D in vitro platform technology to engineer mesenchymal stem cells. Int J Nanomedicine 7:3035–304310.2147/IJN.S30434 ↗
25. Jain TK, Reddy MK, Morales MA, Leslie-Pelecky DL, Labhasetwar V (2008) Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats. Mol Pharm 5:316–32710.1021/mp7001285 ↗
26. Jain S, Thakare VS, Das M, Godugu C, Jain AK, Mathur R, Chuttani K, Mishra AK (2011) Toxicity of multiwalled carbon nanotubes with end defects critically depends on their functionalization density. Chem Res Toxicol 24(11):2028–203910.1021/tx2003728 ↗
27. Kai W, Xiaojun X, Ximing P, Zhenqing H, Qiqing Z (2011) Cytotoxic effects and the mechanism of three types of magnetic nanoparticles on human hepatoma BEL-7402 cells. Nanoscale Res Lett 6:480. doi: 10.1186/1556-276X-6-48010.1186/1556-276X-6-480 ↗
28. Karmali PP, Chao Y, Park JH, Sailor MJ, Ruoslahti E, Esener SC, Simberg D (2012) Different effect of hydrogelation on antifouling and circulation properties of dextran-iron oxide nanoparticles. Mol Pharm 9:539–54510.1021/mp200375x ↗
29. Kim JS, Yoon TJ, Yu KN, Kim BG, Park SJ, Kim HW, Lee KH, Park SB, Lee JK, Cho MH (2006) Toxicity and tissue distribution of magnetic nanoparticles in mice. Toxicol Sci 89:338–34710.1093/toxsci/kfj027 ↗
30. Kim JE, Shin JY, Cho MH (2012) Magnetic nanoparticles: an update of application for drug delivery and possible toxic effects. Arch Toxicol 86(5):685–70010.1007/s00204-011-0773-3 ↗
31. Kosmulski M, Maczka E, Jartych E, Rosenholmb JB (2003) Synthesis and characterization of goethite and goethite–hematite composite: experimental study and literature survey. Adv Colloid Interface Sci 103:57–7610.1016/S0001-8686(02)00083-0 ↗
32. Lee HJ, Park J, Yoon OJ, Kim HW, Lee do Y, Kim do H, Lee WB, Lee NE, Bonventre JV, Kim SS (2011) Amine-modified single-walled carbon nanotubes protect neurons from injury in a rat stroke model. Nat Nanotechnol 6(2):121–12510.1038/nnano.2010.281 ↗
33. Liu G, Swierczewska M, Lee S, Chen X (2010) Functional nanoparticles for molecular imaging guided gene delivery. Nano Today 5:524–53910.1016/j.nantod.2010.10.005 ↗
34. Lu YJ, Wei KC, Ma CC, Yang SY, Chen JP (2012) Dual targeted delivery of doxorubicin to cancer cells using folate-conjugated magnetic multi-walled carbon nanotubes. Colloids Surf B Biointerfaces 89:1–910.1016/j.colsurfb.2011.08.001 ↗
35. Magrez A, Kasas S, Salicio V, Pasquier N, Seo JW, Celio M, Catsicas S, Schwaller B, Forró L (2006) Cellular toxicity of carbon-based nanomaterials. Nano Lett 6:1121–112510.1021/nl060162e ↗
36. Miele E, Spinelli GP, Miele E, Fabrizio ED, Ferretti E, Tomao S, Gulino A (2012) Nanoparticle-based delivery of small interfering RNA: challenges for cancer therapy. Int J Nanomedicine 7:3637–3657
37. Naqvi S, Samim M, Abdin M, Ahmed FJ, Maitra A, Prashant C, Dinda AK (2010) Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress. Int J Nanomedicine 5:983–98910.2147/IJN.S13244 ↗
38. Noh JS, Schwarz JA (1989) Estimation of the point of zero charge of simple oxides by mass titration. J Colloid Interface Sci 130:157–16410.1016/0021-9797(89)90086-6 ↗
39. Novotna B, Jendelova P, Kapcalova M, Rossner P, Turnovcova K, Bagryantseva Y, Babic M, Horak D, Sykova E (2012) Oxidative damage to biological macromolecules in human bone marrow mesenchymal stromal cells labeled with various types of iron oxide nanoparticles. Toxicol Lett 210:53–6310.1016/j.toxlet.2012.01.008 ↗
40. Pan D, Lanza GM, Wickline SA, Caruthers SD (2009) Nanomedicine: perspective and promises with ligand-directed molecular imaging. Eur J Radiol 70:274–28510.1016/j.ejrad.2009.01.042 ↗
41. Park JK, Jung J, Subramaniam P, Shah BP, Kim C, Lee JK, Cho JH, Lee C, Lee KB (2011) Graphite-coated magnetic nanoparticles as multimodal imaging probes and cooperative therapeutic agents for tumor cells. Small 7:1647–165210.1002/smll.201100012 ↗
42. Peng H, Alemany LB, Margrave JL, Khabashesku VN (2003) Sidewall carboxylic acid functionalization of single-walled carbon nanotubes. J Am Chem Soc 125:15174–1518210.1021/ja037746s ↗
43. Poplawska M, Zukowska GZ, Cudzilo S, Bystrzejewski M (2010) Chemical functionalization of carbon-encapsulated magnetic nanoparticles by 1,3-dipolar cycloaddition of nitrile oxide. Carbon 48:1318–132010.1016/j.carbon.2009.11.048 ↗
44. Raoof M, Mackeyev Y, Cheney MA, Wilson LJ, Curley SA (2012) Internalization of C60 fullerenes into cancer cells with accumulation in the nucleus via the nuclear pore complex. Biomaterials 33:2952–296010.1016/j.biomaterials.2011.12.043 ↗
45. Safi M, Courtois J, Seigneuret M, Conjeaud H, Berret JF (2011) The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles. Biomaterials 32:9353–936310.1016/j.biomaterials.2011.08.048 ↗
46. Saltan N, Kutlu HM, Hur D, Iscan A, Say R (2011) Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid. Int J Nanomedicine 6:477–484
47. Schlachter EK, Widmer HR, Bregy A, Lönnfors-Weitzel T, Vajtai I, Corazza N, Bernau VJ, Weitzel T, Mordasini P, Slotboom J, Herrmann G, Bogni S, Hofmann H, Frenz M, Reinert M (2011) Metabolic pathway and distribution of superparamagnetic iron oxide nanoparticles: in vivo study. Int J Nanomedicine 6:1793–1800
48. Shubayev V, Pisanic T, Jin S (2009) Magnetic nanoparticles for theragnostics. Adv Drug Deliv Rev 61(6):467–47710.1016/j.addr.2009.03.007 ↗
49. Singh SK, Singh MK, Nayak MK, Kumari S, Shrivastava S, Grácio JJ, Dash D (2011) Thrombus inducing property of atomically thin graphene oxide sheets. ACS Nano 5(6):4987–499610.1021/nn201092p ↗
50. Singh SK, Singh MK, Kulkarni PP, Sonkar VK, Grácio JJ, Dash D (2012) Amine-modified graphene: thrombo-protective safer alternative to graphene oxide for biomedical applications. ACS Nano 6(3):2731–274010.1021/nn300172t ↗
51. Sohaebuddin SK, Thevenot PT, Baker D, Eaton JW, Tang L (2010) Nanomaterial cytotoxicity is composition, size, and cell type dependent. Part Fibre Toxicol 7:1–1710.1186/1743-8977-7-22 ↗
52. Subramani K, Hosseinkhani H, Khraisat A, Hosseinkhani M, Pathak Y (2009) Targeting nanoparticles as drug delivery systems for cancer treatment. Curr Nanosci 5:134–14010.2174/157341309788185406 ↗
53. Sun YP, Li XQ, Cao J, Zhang WX, Wang HP (2006) Characterization of zero-valent iron nanopartciles. Adv Colloid Interface Sci 120:47–5610.1016/j.cis.2006.03.001 ↗
54. Taylor A, Krupskaya Y, Kramer Y, Fussel S, Klingeler R, Buchner B, Wirth MP (2010) Cisplatin-loaded carbon-encapsulated iron nanoparticles and their in vitro effects in magnetic fluid hyperthermia. Carbon 48:2327–233410.1016/j.carbon.2010.03.009 ↗
55. Tietze R, Lyer S, Dürr S, Alexiou C (2012) Nanoparticles for cancer therapy using magnetic forces. Nanomedicine (Lond) 7:447–45710.2217/nnm.12.10 ↗
56. Tomitaka A, Hirukawa A, Yamada T, Morishita S, Takemura Y (2009) Biocompatibility of various ferrite nanoparticles evaluated by in vitro cytotoxicity assays using HeLa cells. J Mag Magn Mater 321:1482–148410.1016/j.jmmm.2009.02.058 ↗
57. Unfried K, Albrecht C, Klotz LO, Von Mikecz A, Grether-Beck S, Schins RPF (2007) Cellular responses to nanoparticles: target structures and mechanisms. Nanotoxicology 1:52–7110.1080/00222930701314932 ↗
58. Van Beers BE, Sempoux C, Materne R, Delos M, Smith AM (2001) Biodistribution of ultrasmall iron oxide particles in the rat liver. J Magn Reson Imaging 13:594–59910.1002/jmri.1083 ↗
59. Xia G, Chen B, Ding J, Gao C, Lu H, Shao Z, Gao F, Wang X (2011) Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells. Int J Nanomedicine 6:1921–1927
60. Xie J, Lee S, Chen X (2010) Nanoparticle-based theranostic agents. Adv Drug Deliv Rev 62:1064–107910.1016/j.addr.2010.07.009 ↗
61. Xu H, Cheng L, Wang C, Ma X, Li Y, Liu Z (2011) Polymer encapsulated upconversion nanoparticle/iron oxide nanocomposites for multimodal imaging and magnetic targeted drug delivery. Biomaterials 32:9364–937310.1016/j.biomaterials.2011.08.053 ↗
62. Ying E, Hwang HM (2010) In vitro evaluation of the cytotoxicity of iron oxide nanoparticles with different coatings and different sizes in A3 human T lymphocytes. Sci Total Environ 408:4475–448110.1016/j.scitotenv.2010.07.025 ↗
63. Yoo D, Lee JH, Shin TH, Cheon J (2011) Theranostic magnetic nanoparticles. Acc Chem Res 44:863–87410.1021/ar200085c ↗
64. Yu MK, Park J, Jon S (2012) Targeting strategies for multifunctional nanoparticles in cancer imaging and therapy. Theranostics 2(1):3–4410.7150/thno.3463 ↗
65. Yuan Y, Rende D, Altan CL, Bucak S, Ozisik R, Borca-Tasciuc DA (2012) Effect of surface modification on magnetization of iron oxide nanoparticle colloids. Langmuir 28(36):13051–1305910.1021/la3022479 ↗
66. Zeng YY, Chen HJ, Shiau KJ, Hung SU, Wang YS, Wu CC (2012) Efficient enrichment of phosphopeptides by magnetic TiO2-coated carbon-encapsulated iron nanoparticles. Proteomics 12:380–39010.1002/pmic.201000726 ↗
67. Zhang Y, Yang M, Portney NG, Cui D, Budak G, Ozbay E, Ozkan M, Ozkan CS (2008) Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells. Biomed Microdevices 10:321–32810.1007/s10544-007-9139-2 ↗
68. Zhang S, Li J, Lykotrafitis G, Bao G, Suresh S (2009) Size-dependent endocytosis of nanoparticles. Adv Mater 21:419–42410.1002/adma.200801393 ↗
69. Zhu MT, Wang Y, Feng WY, Wang B, Wang M, Ouyang H, Chai ZF (2010) Oxidative stress and apoptosis induced by iron oxide nanoparticles in cultured human umbilical endothelial cells. J Nanosci Nanotechnol 10:8584–859010.1166/jnn.2010.2488 ↗