Preparation of superparamagnetic sodium alginate nanoparticles for covalent immobilization of Candida rugosa lipase
Source abstract
Abstract
Superparamagnetic sodium alginate nanoparticles with diameter around 25–30 nm were prepared with a water-in-oil emulsion method. The resulted magnetic SA nanoparticle was activated with glutaraldehyde and epichlorohydrin to form nanoscale support. Candida rugosa lipase (CRL), hereby chosen as a model enzyme, was covalently immobilized on the resulted magnetic support. The structure and magnetic behavior of the magnetic nanoparticles were confirmed by transmission electron microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Based on the structural character of enzyme (containing functional residues that are ideal reaction sites for the immobilization of enzyme repeatedly), the regeneration of support was investigated by reactivating the deactivated immobilized lipase with glutaraldehyde. And the results indicated that these regenerated supports remained to be efficient for lipase immobilization. Finally, all of the immobilized CRL prepared by different generations of supports displayed excellent reusability and applicability.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Enzyme Catalysis and Immobilization · Innovative Microfluidic and Catalytic Techniques Innovation · Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (15)
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1. Finotelli PV, Morales MA, Rocha-Leao MH, Baggio-Saitovitch EM, Rossi AM (2004) Magnetic studies of iron(III) nanoparticles in alginate polymer for drug delivery applications. Mater Sci Eng C 24:625–62910.1016/j.msec.2004.08.005 ↗
2. Jiang DS, Long SY, Huang J, Xiao HY, Zhou JY (2005) Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres. Biochem Eng J 25:15–2310.1016/j.bej.2005.03.007 ↗
3. Laurienzo P, Malinconico M, Motta A, Vicinanza A (2005) Synthesis and characterization of a novel alginate–poly(ethylene glycol) graft copolymer. Carbohydr Polym 62:274–28210.1016/j.carbpol.2005.08.005 ↗
4. Le-Tien C, Millette M, Lacroix M, Mateescu MA (2004) Modified alginate matrices for the immobilization of bioactive agents. Biotechnol Appl Biochem 39:189–19810.1042/BA20030054 ↗
5. Luo XG, Zhang LN (2010) Immobilization of penicillin G acylase in epoxy-activated magnetic cellulose microspheres for improvement of biocatalytic stability and activities. Biomacromolecules 11:2896–290310.1021/bm100642y ↗
6. Magdy MME, Enas ND, Ghada EAA (2009) Novel carrier of grafted alginate for covalent immobilization of inulinase. Ind Eng Chem Fundam 48:9781–978510.1021/ie9011276 ↗
7. Massart R, Cabuil V (1987) Effect of some parameters on the formation of colloidal magnetite in alkaline-medium-Yield and particle-size control. J Chim Phys Phys Chim Biol 84:967–97310.1051/jcp/1987840967 ↗
8. Mofidi N, Aghai-Moghadam M, Sarbolouki MN (2000) Mass preparation and characterization of alginate microspheres. Process Biochem 35:885–88810.1016/S0032-9592(99)00149-1 ↗
9. Natividad O, Manuel PM, Maria CP, Maria DB (2009) Neutrase immobilization on alginate-glutaraldehyde beads by covalent attachment. J Agric Food Chem 57:109–11510.1021/jf8015738 ↗
10. Tanriseven A, Dogan S (2001) Immobilization of invertase within calcium alginate gel capsules. Process Biochem 36:1081–108310.1016/S0032-9592(01)00146-7 ↗
11. Watanabe N, Ota Y, Minoda Y, Yamada K (1977) Isolation and identification of alkaline lipase producing microorganisms: cultural conditions and some properties of crude enzymes. Agric Biol Chem 41:1353–135810.1271/bbb1961.41.1353 ↗
12. Yeom CK, Lee KH (1998) Characterization of sodium alginate membrane crosslinked with glutaraldehyde in pervaporation separation. J Appl Polym Sci 67:209–21910.1002/(SICI)1097-4628(19980110)67:2<209::AID-APP3>3.0.CO;2-Y ↗
13. Yoshiyuki N, Akiko Y, Kana E, Yoshiharu M, Hidemitsu F, Kazuyuki H (2004) Preparation and magnetometric characterization of iron oxide-containing alginate/poly(vinyl alcohol) networks. Polymer 45:7129–713610.1016/j.polymer.2004.08.047 ↗
14. Zhao GH, Wang JZ, Li YF, Chen X, Liu YP (2011) Enzymes immobilized on superparamagnetic Fe3O4@clays nanocomposites: preparation, characterization, and a new strategy for the regeneration of supports. J Phys Chem C 115:6350–635910.1021/jp200156j ↗
15. Zhu HG, Srivastava R, Brown JQ, Michael JM (2005) Combined physical and chemical immobilization of glucose oxidase in alginate microspheres improves stability of encapsulation and activity. Bioconjugate Chem 16:1451–145810.1021/bc050171z ↗