An investigation of in vivo wound healing activity of biologically synthesized silver nanoparticles
Source abstract
Abstract
Therapeutic use of nano-silver is claimed to have reduced side effects and enhanced curative activity as compared to its ionic counterpart (silver ions). The present work aims to screen microbes for the synthesis of silver nanoparticles (AgNPs), to formulate the nano-silver-based Carbopol gel and evaluating its wound healing efficacy on rat model. The goal was to develop the topical formulation based on bio-nano-silver to control the infection and healing the wounds with higher efficacy. Procedure involved the use of Saccharomyces boulardii for the synthesis of silver nanoparticles in the size range of 3–10 nm and these nanoparticles were used for the preparation of Carbopol-based nano-silver gel. Highly stable Carbopol nanogel was developed with good rheological properties. The burn wound healing potential of this nano-silver gel was evaluated on SD rats via visual observation, transepidermal water loss and histology of skin. Excellent wound healing was observed with AgNPs. Biologically synthesized AgNPs-based nano-silver gel showed superior wound healing efficacy as compared to marketed formulations and silver ions.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Wound Healing and Treatments · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (39)
Show source-provided references
1. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA (2007) Effect of silver on burn wound infection control and healing: review of the literature. Burns 33:139–14810.1016/j.burns.2006.06.010 ↗
2. Barraud C, Cécile Nguon N, Vallet V, Sentenac C, Four E, Arlaud C, Coulon D, Boudry I (2013) Sulfur mustard cutaneous injury characterization based on SKH-1 mouse model: relevance of non-invasive methods in terms of wound healing process analyses. Skin Res Technol 19(1):e146-e1
3. Bjarnsholt T, Kirketerp-Møller K, Jensen PQ, Madsen KG, Phipps R, Krogfelt K, Hoiby N, Givskov M (2008) Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen 16:2–1010.1111/j.1524-475X.2007.00283.x ↗
4. Brandt D, Park B, Hoang M, Jacobe HT (2005) Argyria secondary to ingestion of homemade silver solution. J Am Acad Dermatol 53:105–10710.1016/j.jaad.2004.09.026 ↗
5. Chiara R, Letizia F, Ilaria T, Marco R, Ivan M, Chiara G, Warren RL Cs, Vincenzo V, Bruno A, Carlo B, Barbara Z (2013) Active silver nanoparticles for wound healing. Int J Mol Sci 14:4817–4840. doi: 10.3390/ijms1403481710.3390/ijms14034817 ↗
6. Cho Lee AR, Leem H, Lee J, Park KC (2005) Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor. Biomaterials 26:4670–467610.1016/j.biomaterials.2004.11.041 ↗
7. Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG (2009) A clinical investigation into the microbiological status of’ locally infected’ leg ulcers. Int Wound J 6:453–46210.1111/j.1742-481X.2009.00640.x ↗
8. Davies CE, Hill KE, Newcombe RG, Stephens P, Wilson MJ, Harding KG, Thomas DW (2007) A prospective study of the microbiology of chronic venous leg ulcers to reevaluate the clinical predictive value of tissue biopsies and swabs. Wound Repair Regen 15:17–2210.1111/j.1524-475X.2006.00180.x ↗
9. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 15:662–66810.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3 ↗
10. Fresno Contreras MJ, Ramírez Diéguez A, Jiménez Soriano MM (2001) Rheological characterization of hydroalcoholic gels—15 % ethanol—of Carbopol® Ultrez™ 10. II Farmaco 56:437–44110.1016/S0014-827X(01)01057-6 ↗
11. Gardea-Torresdey JL, Gomez E, Videa-Peralta JR, Parsons JG, Troiani H, Jose-Yacaman M (2003) Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir 19(4):1357–136110.1021/la020835i ↗
12. Grubauer G, Elias PM, Feingold KR (1998) Transepidermal water loss: the signal for recovery of barrier structure and function. J Lipid Res 30:323–33310.1016/S0022-2275(20)38361-9 ↗
13. Gunasekaran T, Nigusse T, Dhanaraju MD (2011) Silver nanoparticles as real topical bullets for wound healing. J Am Coll Clin Wound Spec 3:82–9610.1016/j.jcws.2012.05.001 ↗
14. Gurjala AN, Geringer MR, Seth AK, Hong SJ, Smeltzer MS, Galiano RD, Leung KP, Mustoe TA (2011) Development of a novel, highly quantitative in vivo model for the study of biofilm impaired cutaneous wound healing. Wound Repair Regen 19:400–41010.1111/j.1524-475X.2011.00690.x ↗
15. Innes ME, Umraw N, Fish JS, Gomez M, Cartotto RC (2001) The use of silver coated dressings on donor site wounds: a prospective, controlled matched pair study. Burns 27:621–62710.1016/S0305-4179(01)00015-8 ↗
16. Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13(10):2638–265010.1039/c1gc15386b ↗
17. Jagetia GC, Rajanikant GK, Mallikarjun Rao KVN (2003) Modulation of radiation-induced delay in the wound healing by ascorbic acid in mice exposed to different doses of hemi-body gamma radiation. Wounds 5:10
18. Kaler A, Nankar R, Bhattacharyya MS, Banerjee UC (2011) Extracellular biosynthesis of silver nanoparticles using aqueous extract of Candida viswanathii. J Bionanosci 5:1–610.1166/jbns.2011.1040 ↗
19. Kaler A, Jain S, Banerjee UC (2013) Green and rapid synthesis of anticancerous silver nanoparticles by Saccharomyces boulardii and insight into mechanism of nanoparticle synthesis. BioMed Res Int Volume 2013 Article ID 872940. doi: 10.1155/2013/87294010.1155/2013/872940 ↗
20. Korbekandi H, Iravani S, Abbasi S (2012) Optimization of biological synthesis of silver nanoparticles using Lactobacillus casei subsp. casei. J Chem Tech Biotechnol 87:932–93710.1002/jctb.3702 ↗
21. Lam PK, Chan ES, Ho WS, Liew CT (2004) In vitro cytotoxicity testing of a nanocrystalline silver dressing (Acticoat) on cultured keratinocytes. Br J Biomed Sci 6:125–12710.1080/09674845.2004.11732656 ↗
22. Madsen SM, Westh H, Danielsen L, Rosdahl VT (1996) Bacterial colonization and healing of venous leg ulcers. APMIS 104:895–89910.1111/j.1699-0463.1996.tb04955.x ↗
23. Marambio-Jones C, Hoek EM (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12:1531–155110.1007/s11051-010-9900-y ↗
24. Martinez-Castanon GA, Nino-Martinez N, Martinez-Gutierrez F, Martinez-Mendoza JR, Ruiz F (2008) Synthesis and antibacterial activity of silver nanoparticles with different sizes. J Nanopart Res 10:1343–134810.1007/s11051-008-9428-6 ↗
25. Mittal AK, Kaler A, Banerjee UC (2012) Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from flower extract of Rhododendron dauricum. Nano Biomed Eng 4:118–12410.5101/nbe.v4i3.p118-124 ↗
26. Mittal AK, Chisti Y, Banerjee UC (2013a) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31:346–35610.1016/j.biotechadv.2013.01.003 ↗
27. Mittal AK, Kaler A, Mulay AV, Banerjee UC (2013b) Synthesis of gold nanoparticles using whole cells of Geotrichum candidum. J Nanoparticles. doi: 10.1155/2013/15041410.1155/2013/150414 ↗
28. Mittal AK, Bhaumik J, Kumar S, Banerjee UC (2014) Biosynthesis of silver nanoparticles: Elucidation of prospective mechanism and therapeutic potential. J Coll Interface Sci 415:39–4710.1016/j.jcis.2013.10.018 ↗
29. Nayak RR, Pradhan N, Behera D, Pradhan KM, Mishra S, Sukla LB, Mishra BK (2011) Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization. J Nanopart Res 13:3129–313710.1007/s11051-010-0208-8 ↗
30. Pugazhenthiran N, Anandan S, Kathiravan G, Prakash NKU, Crawford S, Ashok kumar M (2009) Microbial synthesis of silver nanoparticles by Bacillus sp. J Nanopart Res 11:1811–181510.1007/s11051-009-9621-2 ↗
31. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–8310.1016/j.biotechadv.2008.09.002 ↗
32. Salas CL, Fernandes MM, Martinez de la Chica AM (2005) Topical chemotherapy for the treatment of burns. Rev Enferm 28:67–70
33. Samczewska G (2009) The effect of various types of Carbopol and different neutralizing bases on pharmaceutical availability of morphine hydrochloride from hydrogel preparations. Polim Med 39:25–37
34. Shah KA, Date AA, Joshi MD, Patravale VB (2007) Solid lipid nanoparticles (SLN) of tretinoin: potential in topical delivery. Int J Pharm 345:163–17110.1016/j.ijpharm.2007.05.061 ↗
35. Silver S, Phung LT, Silver G (2006) Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds. J Ind Microbiol Biotechnol 33:627–63410.1007/s10295-006-0139-7 ↗
36. Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP (2011) Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart Res 13:2981–298810.1007/s11051-010-0193-y ↗
37. Tian J, Kenneth KYW, Chi-Ming H, Chun-Nam L, Wing-Yiu Y, Chi-Ming C, Jen-Fu C, Paul KHT (2007) Topical delivery of silver nanoparticles promotes wound healing. Chem Med Chem 2:129–13610.1002/cmdc.200600171 ↗
38. Warriner R, Burrell R (2005) Infection and the chronic wound: a focus on silver. Adv Skin Wound Care 18:2–1210.1097/00129334-200510001-00001 ↗
39. Yu A, Guo C, Zhou Y, Cao F, Zhu W, Sun M, Zhai G (2010) Skin irritation and the inhibition effect on HSV-1 in vivo of penciclovir-loaded microemulsion. Int Immunopharmacol 10:1305–130910.1016/j.intimp.2010.07.015 ↗