Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications
Source abstract
Abstract
Bovine femur bone hydroxyapatite (HA) containing silver (Ag) nanoparticles was synthesized by thermal decomposition method and subsequent reduction of silver nitrate with N,N-dimethylformamide (DMF) in the presence of poly(vinylacetate) (PVAc). The structural, morphological, and chemical properties of the HA–Ag nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). TEM images showed that the Ag nanoparticles with size ranging from 8 to 20 nm and were arranged at the periphery of HA crystals. Bactericidal activity of HA–Ag with different concentration of Ag nanoparticles immobilized on the surface of HA was investigated against gram-positive Staphylococcus aureus (S. aureus, non-MRSA), Methicillin resistant S. aureus (MRSA) and gram-negative Escherichia coli (E.coli) by the disc diffusion susceptibility test. The HA–Ag nanoparticles showed that broad spectrum activity against non-MRSA, MRSA, and E. coli bacterial strains.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Nanoparticles: synthesis and applications · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
Show source-provided references
1. Ahmad Z, Pandey R, Sharma S, Khuller GK (2005) Alginate nanoparticles as antituberculosis drug carriers: formulation development, pharmacokinetics and therapeutic potential. Ind J Chest Dis Allied Sci 48:171–176
2. Ando Y, Miyamoto H, Noda I, Sakurai N, Akiyama T, Yonekura Y, Shimazaki T, Miyazaki M, Mawatari M, Hotokebuchi T (2009) Calcium phosphate coating containing silver shows high antibacterial activity and low cytotoxicity and inhibits bacterial adhesion. Mater Sci Eng C 30:175–18010.1016/j.msec.2009.09.015 ↗
3. Barakat NAM, Khalil KA, Sheikh FA, Omran AM, Gaihre B, Khil MS, Kim HY (2008a) Physiochemical characterizations of hydroxyapatite extracted from bovine bones by three different methods: extraction of biologically desirable HAp. Mater Sci Eng C 28:1381–138710.1016/j.msec.2008.03.003 ↗
4. Barakat NM, Woo KD, Kanjwal MA, Choi KE, Khil MS, Kim HY (2008b) Surface plasmon resonances, optical properties, and electrical conductivity thermal hystersis of silver nanofibers produced by the electrospinning technique. Langmuir 24:11982–1198710.1021/la802084h ↗
5. Barakat NAM, Khil MS, Omran AM, Sheik FA, Kim HY (2009) Extraction of pure natural hydroxyapatite from the bovine bones bio waste by three different methods. J Mater Proc Technol 209:3408–341510.1016/j.jmatprotec.2008.07.040 ↗
6. Campoccia D, Montanaro L, Arciola CR (2006) The significance of infection related to orthopedic devices and issues of antibiotic resistance. Biomaterials 27:2331–233910.1016/j.biomaterials.2005.11.044 ↗
7. Chen Y, Zheng X, Xie Y, Ding C, Ruan H, Fan C (2008) Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings. J Mater Sci Mater Med 19:3603–360910.1007/s10856-008-3529-8 ↗
8. Diaz M, Barba F, Miranda M, Guitian F, Torrecillas R, Moya JS (2009) Synthesis and antimicrobial activity of a silver-hydroxyapatite nanocomposite. J Nanomater doi: 10.1155/2009/49850510.1155/2009/498505 ↗
9. Dibrov P, Dzioba J, Gosink KK, Hase CC (2002) Chemiosmotic mechanism of antimicrobial activity of Ag+ in vibrio cholera. Antimicrob Agents Chemother 46:2668–267010.1128/AAC.46.8.2668-2670.2002 ↗
10. Dragieva I, Stoeva S, Stoimenov P, Pavlikianov E, Klabunde K (1999) Complex formation in solutions for chemical synthesis of nanoscaled particles prepared by borohydride reduction process. Nanostruct Mater 12:267–27010.1016/S0965-9773(99)00114-2 ↗
11. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on escherichia coli and staphylococcus aureus. J Biomed Mater Res 52:662–66810.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3 ↗
12. Gong P, Li H, He X, Wang K, Hu J, Tan W, Zhang S, Yang X (2007) Preparation and antibacterial activity of Fe3O4@Ag nanoparticles. Nanotechnology 18:285604–28561110.1088/0957-4484/18/28/285604 ↗
13. Gu H, Ho PL, Tong E, Wang L, Xu B (2003) Presenting vancomycin on nanoparticles to enhance antimicrobial activities. Nano Lett 3:1261–126310.1021/nl034396z ↗
14. Ibarra HO, Casillas N, Soto V, Soto MB, Vitela RT, Cruz W, Saazar SG (2007) Surface characterization of electrodeposited silver on activated carbon for bactericidal purposes. J Colloid Interface Sci 314:562–57110.1016/j.jcis.2007.05.062 ↗
15. Ishikawa K, Ducheyne P, Radin S (1993) Determination of the Ca/P ratio in calcium-deficient hydroxyapatite using X-ray diffraction analysis. J Mater Sci Mater Med 4:165–16810.1007/BF00120386 ↗
16. Jones SA, Bowler PG, Walker M, Parsons D (2004) Controlling wound bioburden with a novel silver-containing Hydrofiber® dressing. Wound Repair Regen 12:288–29410.1111/j.1067-1927.2004.012304.x ↗
17. Joosten U, Joist A, Gosheger G, Liljenqvist U, Brandt B, Eiff CV (2005) Effectiveness of hydroxyapatite–vancomycin bone cement in the treatment of staphylococcus aureus induced chronic osteomyelitis. Biomaterials 26:5251–525810.1016/j.biomaterials.2005.01.001 ↗
18. Jung BO, Lee YM, Kim JJ, Choi YJ, Jung KJ, Chung SJ (1999) The antimicrobial effect of water soluble chitosan. J. Korean Ind Eng Chem 10:660–665
19. Kang HY, Jung MJ, Jeong YK (2000) Antibacterial activity and the stability of an Ag+ solution made using metallic silver. Korean J Biotechnol Bioeng 15:521–524
20. Kim TN, Feng QL, Kim JO, Wu J, Wang H, Chen GC, Cui FA (1998) Antimicrobial effects of metal ions (Ag+, Cu2+, Zn2+) in hydroxyapatite. J Mater Sci Mater Med 9:129–13410.1023/A:1008811501734 ↗
21. Kim JS, Kuk E, Yu KN, Kim JS, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH (2007) Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol Biol Med 3:95–10110.1016/j.nano.2006.12.001 ↗
22. Magana SM, Quintana P, Aguilar DH, Toledo JA, Chavez CA, Cortes MA, Leon L, Pelegrin YF, Lopez T, Sanchez RMT (2008) Antibacterial activity of montmorillonites modified with silver. J Mol Catal A 281:192–19910.1016/j.molcata.2007.10.024 ↗
23. Mastro MA, Hardy AW, Boasso A, Shearer GM, Eddy CR, Kub FJ (2009) Non-toxic inhibition of HIV-1 replication with silver-copper nanoparticles. Med Chem Res doi: 10.1007/s00044-009-9253-110.1007/s00044-009-9253-1 ↗
24. Miura N, Shinohara Y (2009) Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. Biochem Biophys Res Comm 390:733–73710.1016/j.bbrc.2009.10.039 ↗
25. Morones JR, Elechiguerra JL, Camacho A, Ramirez JT (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–235310.1088/0957-4484/16/10/059 ↗
26. Nomura K, Fujii S, Ohki Y, Awazu K, Fujimaki M, Tominaga J, Fukuda N, Hirakawa T, Rockstuhl C (2008) Fabrication of inert silver nanoparticles with a thin silica coating. Jpn J Appl Phys 47:8641–864310.1143/JJAP.47.8641 ↗
27. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–8310.1016/j.biotechadv.2008.09.002 ↗
28. Rameshbabu N, Sampath Kumar TS, Prabhakar TG, Sastry VS, Murty KVGK, Prasad Rao K (2007) Antibacterial nanosized silver substituted hydroxyapatite: synthesis and characterization. J Biomed Mater Res A 80:581–59110.1002/jbm.a.30958 ↗
29. Regi MV (2001) Ceramics for medical applications. J Chem Soc Dalton Trans 4:97–10810.1039/b007852m ↗
30. Samuel L, Turek MD, Lippincott JB (1985) Orthopaedics: principles and applications, 2nd edn. J. B. Lippincott, Philadelphia, pp 113–136
31. Sanosh KP, Chu MC, Balakrishnan A, Lee YJ, Kim TN, Cho SJ (2009) Synthesis of nano hydroxyapatite powder that simulate teeth particle morphology and composition. Curr Appl Phys 9:1459–146210.1016/j.cap.2009.03.024 ↗
32. Santos IP, Marza LML (2002) Synthesis of silver nanoprisms in DMF. Nano Lett 2:903–90510.1021/nl025638i ↗
33. Schabes PSR, Canizal G, Herrera RB, Zorrilla C, Liu HB, Ascencio JA (2006) Biosynthesis and characterization of Ti/Ni bimetallic nanoparticles. Opt Mater 29:95–9910.1016/j.optmat.2006.03.014 ↗
34. Shen Z, Adolfsson E, Nygren M, Gao L, Kawaoka H, Niihara K (2001) Dense hydroxyapatite-zirconia ceramic composites with high strength for biological applications. Adv Mater 13:214–21610.1002/1521-4095(200102)13:3<214::AID-ADMA214>3.0.CO;2-5 ↗
35. Shirkhanzadeh M, Azadegan M, Liu GQ (1995) Bioactive delivery systems for the slow release of antibiotics: incorporation of Ag+ ions into micro-porous hydroxyapatite coatings. Mater Lett 24:7–1210.1016/0167-577X(95)00059-3 ↗
36. Sobhana SSL, Sundaraseelan J, Sekar S, Sastry TP, Mandal AB (2009) Gelatin-chitosan composite capped gold nanoparticles: a matrix for the growth of hydroxyapatite. J Nanopart Res 11:333–34010.1007/s11051-008-9385-0 ↗
37. Sondi I, Sondi BS (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci 275:177–18210.1016/j.jcis.2004.02.012 ↗
38. Tang X, Chen J, Li Y, Li Y, Xu Y, Shen W (2006) Complete oxidation of formaldehyde over Ag/MnOx–CeO2 catalysts. Chem Eng J 118:119–12510.1016/j.cej.2006.02.002 ↗
39. Thian ES, Huang J, Vickers ME, Best SM, Barber ZH, Bonfield W (2006) Silicon-substituted hydroxyapatite (Si-HA): a novel calcium phosphate coating for biomedical applications. J Mater Sci 41:709–71710.1007/s10853-006-6489-8 ↗
40. Tortora GJ, Funke BR, Case CL (1998) Microbiology an introduction, 6th edn. Benjamin/Cummings Publishing Company, California, pp 84–98
41. Wu Y, Jia W, An Q, Liu Y, Chen J, Li G (2009) Multiaction antibacterial nanofibrous membranes fabricated by electrospinning: an excellent system for antibacterial applications. Nanotechnology 20:245101–24510810.1088/0957-4484/20/24/245101 ↗
42. Yen HJ, Hsu SH, Tsai CL (2009) Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. Small 5:1553–156110.1002/smll.200900126 ↗
43. Yeo SG, Ahn CH, Kim IS, Park YB, Park YH, Kim SB (1981) Antimicrobial effect of tea extracts from green tea, oolong tea and black tea. J Korean Soc Food Nutr 24:293–298
44. Zhang C, Yang J, Quan Z, Yang P, Li C, Hou Z, Lin J (2009) Hydroxyapatite nano- and microcrystal with multiform morphologies: controllable synthesis and luminescence properties. Cryst Growth Des 9:2725–273310.1021/cg801353n ↗
45. Zhao K, Feng Q, Chen G (1999) Antimicrobial effects of silver loaded hydroxyapatite. Tsinghua Sci Technol 4:1570–1576