Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells
Source abstract
Abstract
Silver nanoparticles (Ag NPs) are being used increasingly in wound dressings, catheters, and in various household products due to their antimicrobial activity. The present study reports the toxicity evaluation of synthesized and well characterized Ag NPs using Chinese hamster ovary (CHO) cells. The UV–Vis spectroscopy reveals the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 408–410 nm. Transmission electron microscopy (TEM) reveals that the average diameter of silver nanoparticles is about 5.0 ± 1.0 nm and that they have spherical shape. Cell visibility and cell viability percentage show dose-dependent cellular toxicity of Ag NPs. The half maximal inhibitory concentration (IC50) for CHO cells is 68.0 ± 2.65 μg/ml after 24 h Ag NPs exposure. Toxicity evaluations, including cellular morphology, mitochondrial function (MTT assay), reactive oxygen species (ROS), and DNA fragmentation assay (Ladder pattern) were assessed in unexposed CHO cells (control) and the cells exposed to Ag NPs concentrations of 15, 30, and 60 μg/ml for 24 h. The findings may assist in the designing of Ag NPs for various applications and provide insights into their toxicity.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Gold and Silver Nanoparticles Synthesis and Applications · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (66)
Show source-provided references
1. Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ, Hong Y (2008) DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol Appl Pharmacol 233(3):404–410. doi: 10.1016/j.taap.2008.09.01510.1016/j.taap.2008.09.015 ↗
2. Ahsan A, Hiniker SM, Davis MA, Lawrence TS, Nyati MK (2009) Role of cell cycle in epidermal growth factor receptor inhibitor-mediated radiosensitization. Cancer Res 69(12):5108–511410.1158/0008-5472.CAN-09-0466 ↗
3. Arora S, Jain J, Rajwade JM, Paknikar KM (2008) Cellular responses induced by silver nanoparticles: in vitro studies. Toxicol Lett 179(2):93–100. doi: 10.1016/j.toxlet.2008.04.00910.1016/j.toxlet.2008.04.009 ↗
4. AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3(2):279–290. doi: 10.1021/nn800596w10.1021/nn800596w ↗
5. Awasthi KK, John PJ, Awasthi A, Awasthi K (2013) Multi walled carbon nano tubes induced hepatotoxicity in Swiss albino mice. Micron 44:359–364. doi: 10.1016/j.micron.2012.08.00810.1016/j.micron.2012.08.008 ↗
6. Bae CH, Nam SH, Park SM (2002) Formation of silver nanoparticles by laser ablation of a silver target in NaCl solution. Appl Surf Sci 197–198:628–634
7. Bernardi P, Scorrano L, Colonna R, Petronilli V, Di Lisa F (1999) Mitochondria and cell death. Eur J Biochem 264(3):687–70110.1046/j.1432-1327.1999.00725.x ↗
8. Bhakat KK, Mokkapati SK, Boldogh I, Hazra TK, Mitra S (2006) Acetylation of human 8-oxoguanine-DNA glycosylase by p300 and its role in 8-oxoguanine repair in vivo. Mol Cell Biol 26(5):1654–166510.1128/MCB.26.5.1654-1665.2006 ↗
9. Bigall NC, Reitzig M, Naumann W, Simon P, van Pée K-H, Eychmüller A (2008) Fungal templates for noble-metal nanoparticles and their application in catalysis. Angew Chem Int Ed 47(41):7876–787910.1002/anie.200801802 ↗
10. Brause R, Möltgen H, Kleinermanns K (2002) Characterization of laser-ablated and chemically reduced silver colloids in aqueous solution by UV/VIS spectroscopy and STM/SEM microscopy. Appl Phys B 75(6–7):711–716. doi: 10.1007/s00340-002-1024-310.1007/s00340-002-1024-3 ↗
11. Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann M-C (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci 88(2):412–41910.1093/toxsci/kfi256 ↗
12. Braydich-Stolle LK, Lucas B, Schrand A, Murdock RC, Lee T, Schlager JJ, Hussain SM, Hofmann M-C (2010) Silver nanoparticles disrupt GDNF/Fyn kinase signaling in spermatogonial stem cells. Toxicol Sci 116(2):577–58910.1093/toxsci/kfq148 ↗
13. Brayner R (2008) The toxicological impact of nanoparticles. Nano Today 3(1–2):48–55. doi: 10.1016/S1748-0132(08)70015-X10.1016/S1748-0132(08)70015-X ↗
14. Bosques CJ, Collins BE, Meador JW III, Sarvaiya H, Murphy JL, Dellorusso G, Bulik DA, Hsu IH, Washburn N, Sipsey SF, Myette JR, Raman R, Shriver Z, Sasisekharan R, Venkataraman G (2011) Chinese hamster ovary cells can produce galactose-α-1,3-galactose antigens on proteins. Nat Biotechnol 28(11):1153–115610.1038/nbt1110-1153 ↗
15. Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, Schlager JJ (2008) Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 112(43):13608–13619. doi: 10.1021/jp712087m10.1021/jp712087m ↗
16. Chau TT, Campbell JI, Galindo CM, Van Minh H, Diep TS, Nga TTT, Van Vinh Chau N et al (2007) Antimicrobial drug resistance of Salmonella enterica serovar typhi in Asia and molecular mechanism of reduced susceptibility to the fluoroquinolones. Antimicrob Agents Chemother 51(12):4315–432310.1128/AAC.00294-07 ↗
17. Chaudhari VR, Haram SK, Kulshreshtha SK, Bellare JR, Hassan PA (2007) Micelle assisted morphological evolution of silver nanoparticles. Colloids Surf A 301(1–3):475–48010.1016/j.colsurfa.2007.01.025 ↗
18. Croteau M-N, Misra SK, Luoma SN, Valsami-Jones E (2011) Silver bioaccumulation dynamics in a freshwater invertebrate after aqueous and dietary exposures to nanosized and ionic Ag. Environ Sci Technol 45(15):6600–6607. doi: 10.1021/es200880c10.1021/es200880c ↗
19. De Wild M, Berner S, Suzuki H, Ramoino L, Baratoff A, Junga TA (2003) Molecular assembly and self-assemblya: molecular nanoscience for future technologies. Ann N Y Acad Sci 1006(1):291–30510.1196/annals.1292.020 ↗
20. Donaldson K, Stone V, Clouter A, Renwick L, MacNee W (2001) Ultrafine particles. Occup Environ Med 58(3):211–21610.1136/oem.58.3.211 ↗
21. Elechiguerra J, Burt J, Morones J, Camacho-Bragado A, Gao X, Lara H, Yacaman M (2005) Interaction of silver nanoparticles with HIV-1. J Nanobiotechnol 3(1):610.1186/1477-3155-3-6 ↗
22. Zeng F, Hou C, Wu S, Liu X, Tong Z, Yu S (2007) Silver nanoparticles directly formed on natural macroporous matrix and their anti-microbial activities. Nanotechnology 18(5):05560510.1088/0957-4484/18/5/055605 ↗
23. Farkas J, Christian P, Gallego-Urrea JA, Roos N, Hassellöv M, Tollefsen KE, Thomas KV (2011) Uptake and effects of manufactured silver nanoparticles in rainbow trout (Oncorhynchus mykiss) gill cells. Aquat Toxicol 101(1):117–125. doi: 10.1016/j.aquatox.2010.09.01010.1016/j.aquatox.2010.09.010 ↗
24. Foldbjerg R, Dang D, Autrup H (2011) Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Arch Toxicol 85(7):743–750. doi: 10.1007/s00204-010-0545-510.1007/s00204-010-0545-5 ↗
25. Gosheger G, Hardes J, Ahrens H, Streitburger A, Buerger H, Erren M, Gunsel A, Kemper FH, Winkelmann W, von Eiff C (2004) Silver-coated megaendoprostheses in a rabbit model—an analysis of the infection rate and toxicological side effects. Biomaterials 25(24):5547–555610.1016/j.biomaterials.2004.01.008 ↗
26. Haas TA, Plow EF (1994) Integrin–ligarid interactions: a year in review. Curr Opin Cell Biol 6(5):656–662. doi: 10.1016/0955-0674(94)90091-410.1016/0955-0674(94)90091-4 ↗
27. Hallab NJ, Bundy KJ, O’Connor K, Clark R, Moses RL (1995) Cell adhesion to biomaterials: correlations between surface charge, surface roughness, adsorbed protein, and cell morphology. J Long Term Eff Med Implants 5(3):209–231
28. Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19(7):975–983. doi: 10.1016/j.tiv.2005.06.03410.1016/j.tiv.2005.06.034 ↗
29. Kawata K, Osawa M, Okabe S (2009) In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol 43(15):6046–6051. doi: 10.1021/es900754q10.1021/es900754q ↗
30. Kim SYB, Chun K, Kang W, Choo J, Gong M, Joo S (2005) Catalytic effect of laser ablated Ni nanoparticles in the oxidative addition reaction for a coupling reagent of benzylchloride and bromoacetonitrile. J Mol Catal A 226:231–23410.1016/j.molcata.2004.10.038 ↗
31. Kim S, Choi JE, Choi J, Chung KH, Park K, Yi J, Ryu DY (2009) Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In Vitro 23(6):1076–108410.1016/j.tiv.2009.06.001 ↗
32. Kim S-H, Lee H-S, Ryu D-S, Choi S-J, Lee D-S (2011) Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli. Korean J Microbiol Biotechnol 39(1):77–85
33. Kumar A, Joshi H, Pasricha R, Mandale AB, Sastry M (2003) Phase transfer of silver nanoparticles from aqueous to organic solutions using fatty amine molecules. J Colloid Interface Sci 264(2):396–40110.1016/S0021-9797(03)00567-8 ↗
34. Li G-Y, Osborne NN (2008) Oxidative-induced apoptosis to an immortalized ganglion cell line is caspase independent but involves the activation of poly(ADP-ribose)polymerase and apoptosis-inducing factor. Brain Res 1188:35–43. doi: 10.1016/j.brainres.2007.10.07310.1016/j.brainres.2007.10.073 ↗
35. Li DG, Chen SH, Zhao SY, Hou XM, Ma HY, Yang XG (2004) Simple method for preparation of cubic Ag nanoparticles and their self-assembled films. Thin Solid Films 460(1–2):78–82
36. Liu S, Zhang Z, Han M (2005) Gram-scale synthesis and biofunctionalization of silica-coated silver nanoparticles for fast colorimetric DNA detection. Anal Chem 77(8):2595–2600. doi: 10.1021/ac048286410.1021/ac0482864 ↗
37. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–6310.1016/0022-1759(83)90303-4 ↗
38. Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12(3):788–800. doi: 10.1021/la950271110.1021/la9502711 ↗
39. Paddle-Ledinek JE, Nasa Z, Cleland HJ (2006) Effect of different wound dressings on cell viability and proliferation. Plast Reconstr Surg 117(7S):110S–118S10.1097/01.prs.0000225439.39352.ce ↗
40. Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73(6):1712–172010.1128/AEM.02218-06 ↗
41. Panda KK, Achary VMM, Krishnaveni R, Padhi BK, Sarangi SN, Sahu SN, Panda BB (2011) In vitro biosynthesis and genotoxicity bioassay of silver nanoparticles using plants. Toxicol In Vitro 25(5):1097–1105. doi: 10.1016/j.tiv.2011.03.00810.1016/j.tiv.2011.03.008 ↗
42. Park E-J, Yi J, Kim Y, Choi K, Park K (2010) Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicol In Vitro 24(3):872–878. doi: 10.1016/j.tiv.2009.12.00110.1016/j.tiv.2009.12.001 ↗
43. Patel K, Kapoor S, Dave DP, Mukherjee T (2005) Synthesis of Pt, Pd, Pt/Ag and Pd/Ag nanoparticles by microwave-polyol method. J Chem Sci 117(4):311–31610.1007/BF02708443 ↗
44. Paz-Elizur T, Krupsky M, Blumenstein S, Elinger D, Schechtman E, Livneh Z (2003) DNA repair activity for oxidative damage and risk of lung cancer. J Natl Cancer Inst 95(17):1312–131910.1093/jnci/djg033 ↗
45. Piao MJ, Kang KA, Lee IK, Kim HS, Kim S, Choi JY, Choi J, Hyun JW (2011) Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett 201(1):92–100. doi: 10.1016/j.toxlet.2010.12.01010.1016/j.toxlet.2010.12.010 ↗
46. Pillai ZS, Kamat PV (2003) What factors control the size and shape of silver nanoparticles in the citrate ion reduction method? J Phys Chem B 108(3):945–951. doi: 10.1021/jp037018r10.1021/jp037018r ↗
47. Ringer SP, Ratinac KR (2004) On the role of characterization in the design of interfaces in nanoscale materials technology. Microsc Microanal 10(03):324–33510.1017/S1431927604040504 ↗
48. Salkar RA, Jeevanandam PT, Aruna S, Koltypin Y, Gedanken A (1999) The sonochemical preparation of amorphous silver nanoparticles. J Mater Chem 9(6):1333–133510.1039/a900568d ↗
49. Samuel U, Guggenbichler JP (2004) Prevention of catheter-related infections: the potential of a new nano-silver impregnated catheter. Int J Antimicrob Agents 23:75–7810.1016/j.ijantimicag.2003.12.004 ↗
50. Sanpui P, Chattopadhyay A, Ghosh SS (2011) Induction of apoptosis in cancer cells at low silver nanoparticle concentrations using chitosan nanocarrier. ACS Appl Mater Interfaces 3(2):218–228. doi: 10.1021/am100840c10.1021/am100840c ↗
51. Schins RPF, Knaapen AM (2007) Genotoxicity of poorly soluble particles. Inhalation Toxicol 19(s1):189–198. doi: 10.1080/0895837070149620210.1080/08958370701496202 ↗
52. Schrand AM, Braydich-Stolle LK, Schlager JJ, Dai L, Hussain SM (2008) Can silver nanoparticles be useful as potential biological labels? Nanotechnology 19:23510410.1088/0957-4484/19/23/235104 ↗
53. Sheeley DM, Merrill BM, Taylor LCE (1997) Characterization of monoclonal antibody glycosylation: comparison of expression systems and identification of terminal α-linked galactose. Anal Biochem 247(1):102–110. doi: 10.1006/abio.1997.203610.1006/abio.1997.2036 ↗
54. Soroushian BLI, Belloni J, Mostafavi M (2005) Radiolysis of silver ion solutions in ethylene glycol: solvated electron and radical scavenging yields. Radiat Phys Chem 72:111–11810.1016/j.radphyschem.2004.02.009 ↗
55. Sosa IO, Noguez C, Barrera RG (2003) Optical properties of metal nanoparticles with arbitrary shapes. J Phys Chem B 107(26):6269–6275. doi: 10.1021/jp027407610.1021/jp0274076 ↗
56. Starowicz M, Stypuła B, Banaś J (2006) Electrochemical synthesis of silver nanoparticles. Electrochem Commun 8(2):227–23010.1016/j.elecom.2005.11.018 ↗
57. Suber L, Sondi I, Matijević E, Goia DV (2005) Preparation and the mechanisms of formation of silver particles of different morphologies in homogeneous solutions. J Colloid Interface Sci 288(2):489–49510.1016/j.jcis.2005.03.017 ↗
58. Sun Y, Xia Y (2002) Shape-controlled synthesis of gold and silver nanoparticles. Science 298(5601):2176–217910.1126/science.1077229 ↗
59. Tran QH, Nguyen VQ, Le AT (2013) Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Adv Nat Sci Nanosci Nanotechnol 4:033001–03302010.1088/2043-6262/4/3/033001 ↗
60. Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM, Balasubramanya RH (2007) Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett 61(6):1413–1418. doi: 10.1016/j.matlet.2006.07.04210.1016/j.matlet.2006.07.042 ↗
61. Vinardell MP (2005) In vitro cytotoxicity of nanoparticles in mammalian germ-line stem cell. Toxicol Sci 88(2):285–28610.1093/toxsci/kfi340 ↗
62. Wise JP Sr, Goodale BC, Wise SS, Craig GA, Pongan AF, Walter RB, Thompson WD, Ng A-K, Aboueissa A-M, Mitani H, Spalding MJ, Mason MD (2010) Silver nanospheres are cytotoxic and genotoxic to fish cells. Aquat Toxicol 97(1):34–41. doi: 10.1016/j.aquatox.2009.11.01610.1016/j.aquatox.2009.11.016 ↗
63. Xie Y, Ye R, Liu H (2006) Synthesis of silver nanoparticles in reverse micelles stabilized by natural biosurfactant. Colloids Surf A 279(1–3):175–17810.1016/j.colsurfa.2005.12.056 ↗
64. Yavuz Basterzi GE, Sarac G, Sari A, Demirkan F (2010) In vitro comparison of antimicrobial efficacy of various wound dressing materials. Wounds 22(7):165–170
65. Zhang J-p, Chen P, Sun C-h, Hu X-j (2004) Sonochemical synthesis of colloidal silver catalysts for reduction of complexing silver in DTR system. Appl Catal A 266(1):49–5410.1016/j.apcata.2004.01.025 ↗
66. Zhang W, Qiao X, Chen J (2008) Formation of silver nanoparticles in SDS inverse microemulsions. Mater Chem Phys 109(2–3):411–41610.1016/j.matchemphys.2007.12.009 ↗