Nanodiamond internalization in cells and the cell uptake mechanism
Source abstract
Abstract
Cell type-dependent penetration of nanodiamond in living cells is one of the important factors for using nanodiamond as cellular markers/labels, for drug delivery as well as for other biomedical applications. In this work, internalization of 100 nm nanodiamonds by A549 lung human adenocarcinoma cell, Beas-2b non-tumorigenic human bronchial epithelial cell, and HFL-1 fibroblast-like human fetal lung cell is studied and compared. The penetration of nanodiamond into the cells was observed using confocal fluorescence imaging and Raman imaging methods. Visualization of the nanodiamond in cells allows comparison of the internalization for diamond nanoparticles in cancer A549 cell, non-cancer HFL-1, and Beas-2b cells. The dose-dependent and time-dependent behavior of nanodiamond uptake is observed in both cancer as well as non-cancer cells. The mechanism of nanodiamond uptake by cancer and non-cancer cells is analyzed by blocking different pathways. The uptake of nanodiamond in both cancer and non-cancer cells was found predominantly via clathrin-dependent endocytosis. In spite of observed similarity in the uptake mechanism for cancer and non-cancer cells, the nanodiamond uptake for cancer cell quantitatively exceeds the uptake for non-cancer cells, for the studied cell lines. The observed difference in internalization of nanodiamond by cancer and non-cancer cells is discussed.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Diamond and Carbon-based Materials Research · Ion-surface interactions and analysis · Mass Spectrometry Techniques and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (48)
Show source-provided references
1. Agrawal UP (2006) Raman imaging to investigate ultrastructure and composition of plant cell walls: distribution of lignin and cellulose in black spruce wood (Picea mariana). Planta 224:1141–115310.1007/s00425-006-0295-z ↗
2. Barua S, Rege K (2009) Cancer cell-phenotype-dependent differential intracellular trafficking of unconjugated quantum dots. Small 5:370–37610.1002/smll.200800972 ↗
3. Bauer IW, Li S-P, Han Y-C, Yuan L, Yin M-Z (2008) Internalization of hydroxyapatite nanoparticles in liver cancer cells. J Mater Sci Mater Med 19:1091–109510.1007/s10856-007-3124-4 ↗
4. Bema D, Freivalds T, Buikis I, Harju L (2008) Microcells development and endocytosis ability morphological and quantitative characterization in HeLa cancer cells. IFMBE Proc 20:598–60110.1007/978-3-540-69367-3_159 ↗
5. Busch W, Bastian S, Trahorsch U, Iwe M, Kuehnel D, Meißner T, Springer A, Gelinsky M, Richter V, Ikonomidou C, Potthoff A, Lehmann I, Schirmer K (2011) Internalisation of engineered nanoparticles into mammalian cells in vitro: influence of cell type and particle properties. J Nanopart Res 13:293–31010.1007/s11051-010-0030-3 ↗
6. Chang CC, Chen PH, Chu HL, Lee TC, Chou CC, Chao JI, Su CY, Chen JS, Tsai JS, Tsai CM, Ho YP, Sun KW, Cheng CL, Chen FR (2008) Laser induced popcornlike conformational transition of nanodiamond as a nanoknife. Appl Phys Lett 93:03390510.1063/1.2955840 ↗
7. Chao JI, Perevedentseva E, Chung PH, Liu KK, Cheng CY, Chang CC, Cheng CL (2007) Nanometer-sized diamond particle as a probe for biolabeling. Biophys J 93:2199–220810.1529/biophysj.107.108134 ↗
8. Chen HH, Chien CC, Petibois C, Wang CL, Chu YS, Lai SF, Hua TE, Chen YY, Cai X, Kempson IM, Hwu Y, Margaritondo G (2011) Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy. J Nanobiotechnol 9:1410.1186/1477-3155-9-14 ↗
9. Cheng CY, Perevedentseva E, Tu JS, Chung PH, Cheng CL, Liu KK, Chao JI, Chen PH, Chang CC (2007) Appl Phys Lett 90:16390310.1063/1.2727557 ↗
10. Chou LYT, Ming K, Chan WCW (2011) Strategies for the intracellular delivery of nanoparticles. Chem Soc Rev 40:233–24510.1039/C0CS00003E ↗
11. Chung PH, Perevedentseva E, Tu JS, Chang CC, Cheng C-L (2006) Spectroscopic study of bio-functionalized nanodiamonds. Diam Relat Mater 15:622–62510.1016/j.diamond.2005.11.019 ↗
12. Cuche A, Sonnefraud Y, Faklaris O, Garrot D, Boudou JP, Sauvage T, Roch JF, Treussart F, Huant S (2009) Diamond nanoparticles as photoluminescent nanoprobes for biology and near-field optics. J Luminesc 129:1475–147710.1016/j.jlumin.2009.04.089 ↗
13. Defrus AM, Chan WCM, Bhatia SN (2004) Intracellular delivery of quantum dots for life cell labeling and organelle tracking. Adv Mater 6:691–696
14. Faklaris O, Garrot D, Joshi V, Druon F, Boudou JP, Sauvage T, Georges P, Curmi PA, Treussart F (2008) Detection of single photoluminescent diamond nanoparticles in cells and study of the internalization pathway. Small 4:2236–223910.1002/smll.200800655 ↗
15. Faklaris O, Joshi V, Irinopoulou T, Tauc P, Girard H, Gesset C, Senour M, Thorel A, Arnault JC, Boudou JP, Curmi PA, Treussart F (2009) Determination of the internalization pathway of photoluminescent nanodiamonds in mammalian cells for biological labeling and optimization of the fluorescent yield. ASC Nano 3:3955–396210.1021/nn901014j ↗
16. Fang CY, Vaijayanthimala V, Cheng CA, Yeh SH, Chang CF, Li CL, Chang HC (2011) The exocytosis of fluorescent nanodiamond and its use as a long-term cell tracker. Small 7:3363–337010.1002/smll.201101233 ↗
17. Floyd S, De Camilli P (1998) Endocytosis proteins and cancer: a potential link? Trends Cell Biol 8:299–30110.1016/S0962-8924(98)01316-6 ↗
18. Fu CC, Lee HY, Chen K, Lim TS, Wu HY, Lin PK, Wei PK, Tsao PH, Chang HC, Fann W (2007) Characterization and application of single fluorescent nanodiamonds as cellular biomarkers. Proc Natl Acad Sci USA 104:727–73210.1073/pnas.0605409104 ↗
19. Geiser M, Rothen-Rutishauser B, Kapp N, Schürch S, Kreyling W, Schulz H, Semmler M, Im Hof V, Heyder J, Gehr P (2005) Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells. Environ Health Persp 113:1555–156010.1289/ehp.8006 ↗
20. Goodman TT, Chen J, Matveev K, Pun SH (2008) Spatio-temporal modeling of nanoparticle delivery to multicellular tumor spheroids. Biotechnol Bioeng 101:388–39910.1002/bit.21910 ↗
21. Grichko V, Grishko V, Shenderova O (2006) Nanodiamond bullets and their biological targets. NanoBiotech 2:37–4210.1007/s12030-006-0005-8 ↗
22. Gullotti E, Yeo Y (2009) Extracellularly activated nanocarriers: a new paradigm of tumor targeted drug delivery. Mol Pharm 6:1041–105110.1021/mp900090z ↗
23. Harush-Frenkel O, Debotton N, Benita S, Altschuler Y (2007) Targeting of nanoparticles to the clathrin-mediated endocytic pathway. Biochem Biophys Res Comm 353:26–3210.1016/j.bbrc.2006.11.135 ↗
24. Hillaireau H, Couvreur P (2009) Nanocarriers’ entry into the cell: relevance to drug delivery. Cell Mol Life Sci 66:2873–289610.1007/s00018-009-0053-z ↗
25. Huang H, Pierstorff E, Osava E, Ho D (2007) Active nanodiamond hydrogels for chemotherapeutic delivery. Nano Lett 7:3305–331410.1021/nl071521o ↗
26. Iversen TG, Skotland T, Sandvig K (2011) Endocytosis and intracellular transport of nanoparticles: present knowledge and need for future studies. Nano Today 6:176–18510.1016/j.nantod.2011.02.003 ↗
27. Kim JA, Åberg C, Salvati A, Dawson KA (2012) Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population. Nat Nanotechnol 7:62–6810.1038/nnano.2011.191 ↗
28. Krueger A (2008) New carbon materials: biological applications of functionalized nanodiamond. Mater Chem Eur J 14:1382–139010.1002/chem.200700987 ↗
29. Lien ZY, Hsu TC, Liu KK, Liao WS, Hwang KC, Chao JI (2012) Cancer cell labeling and tracking using fluorescent and magnetic nanodiamond. Biomaterials 33:6172–618510.1016/j.biomaterials.2012.05.009 ↗
30. Liu KK, Cheng CL, Chang CC, Chao JI (2007) Biocompatible and detectable carboxylated nanodiamond on human cell. Nanotechnology 18:32510210.1088/0957-4484/18/32/325102 ↗
31. Liu KK, Chen MF, Chen PY, Lee TJF, Cheng CL, Chang CC, Ho YP, Chao JI (2008) Alpha-bungarotoxin binding to target cell in a developing visual system by carboxylated nanodiamond. Nanotechnology 19:20510210.1088/0957-4484/19/20/205102 ↗
32. Liu KK, Wang CC, Cheng CL, Chao JI (2009) Endocytic carboxylated nanodiamond for the labeling and tracking of cell division and differentiation in cancer and stem cells. Biomaterials 30:4249–425910.1016/j.biomaterials.2009.04.056 ↗
33. Liu KK, Zheng WW, Wang CC, Chiu YC, Cheng CL, Lo YS, Chen C, Chao JI (2010) Covalent linkage of nanodiamond paclitaxel for drug delivery and cancer therapy. Nanotechnology 21:31510610.1088/0957-4484/21/31/315106 ↗
34. Loh O, Lam R, Chen M, Moldovan N, Huang H, Ho D, Espinosa HD (2009) Nanofountain-probe-based high-resolution patterning and single-cell injection of functionalized nanodiamonds. Small 5:1667–167410.1002/smll.200900361 ↗
35. Maeda H (2001) The enhanced permeability and retention (EPR) effect in tumor vasculature: the key role of tumor-selective macromolecular drug targeting. Advan Enzyme Regul 41:189–20710.1016/S0065-2571(00)00013-3 ↗
36. Perevedentseva E, Cheng CY, Chung PH, Tu JS, Hsieh YH, Cheng CL (2007) The interaction of the protein lysozyme with bacteria E. coli observed using nanodiamond labeling. Nanotechnology 18:31510210.1088/0957-4484/18/31/315102 ↗
37. Polo S, Pece S, Di Fiore PP (2004) Endocytosis and cancer. Curr Opinion Cell Biol 16:156–16110.1016/j.ceb.2004.02.003 ↗
38. Rejman J, Oberle V, Zuhorn IS, Hoekstra D (2004) Size-dependent internalization of particles via the pathways of clathrin and caveolae-mediated endocytosis. Biochem J 377:159–16910.1042/bj20031253 ↗
39. Sahay G, Oh Kim J, Kabanov AV, Bronich TK (2010) The exploitation of differential endocytic pathways in normal and tumor cells in the selective targeting of nanoparticulate chemotherapeutic age. Biomaterials 31:923–93310.1016/j.biomaterials.2009.09.101 ↗
40. Schrand AM, Huang H, Carlson C, Schlager JJ, Osawa E, Hussain SM, Dai L (2007) Are diamond nanoparticles cytotoxic? J Phys Chem B 111:2–710.1021/jp066387v ↗
41. Sun W, Fang N, Trewyn BGT, Tsunoda M, Slowing II, Lin VSY, Yeung ES (2008) Endocytosis of a single mesoporous silica nanoparticle into a human lung cancer cell observed by differential interference contrast microscopy. Anal Bioanal Chem 391:2119–212510.1007/s00216-008-2162-1 ↗
42. Thomas V, Halloran BA, Ambalavanan N, Catledge SA, Vohra YK (2012) In vitro studies on the effect of particle size on macrophage responses to nanodiamond wear debris. Acta Biomater 8:1939–194710.1016/j.actbio.2012.01.033 ↗
43. Tsai SW, Liaw JW, Hsu FH, Chen YY, Lyu MJ, Yeh MH (2008) Surface-modified gold nanoparticles with folic acid as optical probes for cellular imaging. Sensors 8:6660–667310.3390/s8106660 ↗
44. Verma A, Stellacci F (2010) Effect of surface properties on nanoparticle-cell interactions. Small 6:12–2110.1002/smll.200901158 ↗
45. Vicent MJ, Duncan R (2006) Polymer conjugates: nanosized medicines for treating cancer. Trends Biotechnol 24:39–4710.1016/j.tibtech.2005.11.006 ↗
46. Wang J, Zhan Y, Bao N, Lu C (2012) Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry. Lab Chip 12:1441–144510.1039/c2lc21298f ↗
47. Wee TL, Mau YM, Fang CY, Hsu HL, Han CC, Chang HC (2009) Preparation and characterization of green fluorescent nanodiamonds for biological applications. Diam Relat Mater 18:567–57310.1016/j.diamond.2008.08.012 ↗
48. Zhang LW, Monteiro-Riviere NA (2009) Mechanisms of quantum dot nanoparticle cellular uptake. Toxicol Sci 110:138–15510.1093/toxsci/kfp087 ↗