Influence of hydroxyapatite nanoparticle shape on carrier concentration and its role in osteosarcoma cell inhibition
Source abstract
Abstract
Abstract In this study, two distinct hydroxyapatite nanoparticle (HAp) shapes—nanospheres and nanorods—were synthesized and evaluated for their influence on cytotoxicity and photocatalysis using MG63 osteosarcoma cells. The standard chemical precipitation process was used to create the hydroxyapatite nanospheres, while polyvinyl alcohol was used as a capping agent to create the nanorod forms. The characteristic features of the fabricated hydroxyapatite nanoparticles (HAp) were examined with X-ray diffraction (XRD) analysis, Raman spectroscopy, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and photoluminescence (PL). Also, high-resolution transmission electron microscopy (TEM) images and positron annihilation lifetime spectroscopy (PALS) were used to understand the relationship between the defects in hydroxyapatite (HAp) samples and the photodegradation of the MB dye. The interfacial charge-transfer behavior for each sample in an electrolyte was characterized by Mott–Schottky and electrochemical impedance. Also, the carrier density and conductivity of the manufactured materials and lattice structures were observed in two shapes, confirming the crystalline nature of the nanoparticles. To assess cytotoxicity, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using the osteosarcoma cell line (MG63). Nanorod particles exhibited higher cytotoxicity than nanospheres, as the application of nanotechnology starts to be an effective tool in cancer treatment. The outcomes of the current study may assist in the shape design of hydroxyapatite nanoparticles for therapeutic applications such as drug delivery vehicles in nanomedicine. It highlights the strong correlation between the electrochemical impedance and the cytotoxicity of the hydroxyapatite (HAp) samples, elucidating the role of charge carriers in cytotoxicity and photocatalytic degradation, initiating the redox reaction which releases the toxic reactive species selectively within osteosarcoma cells, resulting in the destruction of cancer cells while sparing normal cells, and demonstrating how physical and electrical characteristics impact a range of applications. Graphical Abstract
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · Planarian Biology and Electrostimulation · Hydrogen's biological and therapeutic effects
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References (100+)
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1. Zheng Y, Jiang W, Chen D, Wang L, Li Y, Dai L et al (2020) Research progress on exosome in malignant tumors. Chin J Lung Cancer 23:689–694. https://doi.org/10.3779/j.issn.1009-3419.2020.101.2810.3779/j.issn.1009-3419.2020.101.28 ↗
2. Zeien J, Qiu W, Triay M, Dhaibar HA, Cruz-Topete D, Cornett EM et al (2022) Clinical implications of chemotherapeutic agent organ toxicity on perioperative care. Biomed Pharmacother 146:112503. https://doi.org/10.1016/j.biopha.2021.11250310.1016/j.biopha.2021.112503 ↗
3. Zhou Z, Li M (2022) Targeted therapies for cancer. BMC Med 20:1–3. https://doi.org/10.1186/s12916-022-02287-310.1186/s12916-022-02287-3 ↗
4. Li J, Wang Q, Xia G, Adilijiang N, Li Y, Hou Z et al (2023) Recent advances in targeted drug delivery strategy for enhancing oncotherapy. Pharmaceutics. https://doi.org/10.3390/pharmaceutics1509223310.3390/pharmaceutics15092233 ↗
5. Ejigah V, Owoseni O, Bataille-Backer P, Ogundipe OD, Fisusi FA, Adesina SK (2022) Approaches to improve macromolecule and nanoparticle accumulation in the tumor microenvironment by the enhanced permeability and retention effect. Polymers. https://doi.org/10.3390/polym1413260110.3390/polym14132601 ↗
6. Vafa E, Azizli MJ, Tayebi L, Amani AM, Kamyab H (2024) Integrating bioactive glass with compatibilizer: a novel approach for hard tissue regeneration using PXDDA-co-PLA/PLCL/PGLA nanocomposites. Mater Chem Phys 316:316. https://doi.org/10.1016/j.matchemphys.2024.12907010.1016/j.matchemphys.2024.129070 ↗
7. Alshammari BH, Lashin MMA, Mahmood MA, Al-Mubaddel FS, Ilyas N, Rahman N et al (2023) Organic and inorganic nanomaterials: fabrication, properties and applications. RSC Adv 13:13735–13785. https://doi.org/10.1039/d3ra01421e10.1039/d3ra01421e ↗
8. Kargozar S, Mollazadeh S, Kermani F, Webster TJ, Nazarnezhad S, Hamzehlou S et al (2022) Hydroxyapatite nanoparticles for improved cancer theranostics. J Funct Biomater. https://doi.org/10.3390/jfb1303010010.3390/jfb13030100 ↗
9. Vafa E, Tayebi L, Azizli F, Parham S, Rezaeeparto K, Azadi S et al (2025) Effect of bioactive glass on PXDDA / PXDDA-co-PLA nanocomposite for hard tissue reconstruction: synthesis and characterization. J Mater Res Technol 36:4773–4785. https://doi.org/10.1016/j.jmrt.2025.04.05510.1016/j.jmrt.2025.04.055 ↗
10. Mo X, Zhang D, Liu K, Zhao X, Li X, Wang W (2023) Nano-hydroxyapatite composite scaffolds loaded with bioactive factors and drugs for bone tissue engineering. Int J Mol Sci. https://doi.org/10.3390/ijms2402129110.3390/ijms24021291 ↗
11. De Lama-Odría MdelC, del Valle LJ, Puiggalí J (2022) Hydroxyapatite biobased materials for treatment and diagnosis of cancer. Int J Mol Sci. https://doi.org/10.3390/ijms23191135210.3390/ijms231911352 ↗
12. Fukada M, Chhetri T, Suresh A, Upendran A, Afrasiabi Z (2023) Size and morphology-mediated antiproliferative activity of hydroxyapatite nanoparticles in human breast cancer cells. J Nanotechnol. https://doi.org/10.1155/2023/538115810.1155/2023/5381158 ↗
13. Asgharzadeh F, Hashemzadeh A, Eskandari M, Yaghoubi A, Naghibzadeh N, Mostafapour A et al (2022) Synthesis and evaluation of anti-inflammatory properties of hydroxyapatite nanoparticles in an experimental model of colitis. Nanomedicine J 9:319–327. https://doi.org/10.22038/NMJ.2022.65906.169110.22038/NMJ.2022.65906.1691 ↗
14. Azizli MJ, Lashgari S, Rezaeeparto K, Parham S, Ghadami A, Tayebi L et al (2025) Polymer synergy: enhancing PA6/PLA properties with POE-g-MA and bioactive glass for advanced biomedical solutions. J Polym Environ 33:1947–1971. https://doi.org/10.1007/s10924-025-03505-810.1007/s10924-025-03505-8 ↗
15. Furko M, Balázsi K, Balázsi C (2023) Calcium phosphate loaded biopolymer composites—a comprehensive review on the most recent progress and promising trends. Coatings. https://doi.org/10.3390/coatings1302036010.3390/coatings13020360 ↗
16. Trzaskowska M, Vivcharenko V, Przekora A (2023) The impact of hydroxyapatite sintering temperature on its microstructural, mechanical, and biological properties. Int J Mol Sci. https://doi.org/10.3390/ijms2406508310.3390/ijms24065083 ↗
17. Agbeboh NI, Oladele IO, Daramola OO, Adediran AA, Olasukanmi OO, Tanimola MO (2020) Environmentally sustainable processes for the synthesis of hydroxyapatite. Heliyon. https://doi.org/10.1016/j.heliyon.2020.e0376510.1016/j.heliyon.2020.e03765 ↗
18. Ismail W, Ibrahim G, Habib MA, Alduaij OK, Abdelfatah M, El-Shaer A (2023) Advancement of physical and photoelectrochemical properties of nanostructured CdS thin films toward optoelectronic applications. Nanomaterials. https://doi.org/10.3390/nano1311176410.3390/nano13111764 ↗
19. Lopes R, Gordo PM, Costa BFO, Alves P (2024) Formulation and characterization of chitosan-based mixed-matrix scaffold for tissue engineering. Macromol 4:253–268. https://doi.org/10.3390/macromol402001410.3390/macromol4020014 ↗
20. Kankala RK, Han YH, Xia HY, Wang SB, Chen AZ (2022) Nanoarchitectured prototypes of mesoporous silica nanoparticles for innovative biomedical applications. J Nanobiotechnology. https://doi.org/10.1186/s12951-022-01315-x10.1186/s12951-022-01315-x ↗
21. Gupta S, Attar ES, Joshi V, Devarajan PV (2025) Effect of shape on cellular internalization and anti-cancer efficacy of hydroxyapatite nanoparticles in an osteosarcoma cell line. RSC Pharm. https://doi.org/10.1039/d5pm00005j10.1039/d5pm00005j ↗
22. Gervasi F, Pojero F (2024) Use of oleuropein and hydroxytyrosol for cancer prevention and treatment: considerations about how bioavailability and metabolism impact their adoption in clinical routine. Biomedicines. https://doi.org/10.3390/biomedicines1203050210.3390/biomedicines12030502 ↗
23. Esfahrood ZR, Sarlak H, Torshabi M, Hartoonian S, Shekarchi F (2025) Assessment of photobiomodulation effect on osteoblast-like MG-63 cell biological behavior: analyzing proliferation and migration in the absence or presence of nicotine. BMC Oral Health. https://doi.org/10.1186/s12903-025-06386-410.1186/s12903-025-06386-4 ↗
24. Theansungnoen T, Nitthikan N, Wilai M, Chaiwut P, Kiattisin K, Intharuksa A (2022) Phytochemical analysis and antioxidant, antimicrobial, and antiaging activities of ethanolic seed extracts of four mucuna species. Cosmet. https://doi.org/10.3390/cosmetics901001410.3390/cosmetics9010014 ↗
25. Nasrallah DA, Ibrahim MA (2022) Enhancement of physico-chemical, optical, dielectric and antimicrobial properties of polyvinyl alcohol/carboxymethyl cellulose blend films by addition of silver doped hydroxyapatite nanoparticles. J Polym Res. https://doi.org/10.1007/s10965-022-02943-510.1007/s10965-022-02943-5 ↗
26. Rabiei M, Palevicius A, Monshi A, Nasiri S, Vilkauskas A, Janusas G (2020) Comparing methods for calculating nano crystal size of natural hydroxyapatite using X-ray diffraction. Nanomaterials 10:1–21. https://doi.org/10.3390/nano1009162710.3390/nano10091627 ↗
27. Neves JG, Navarro da Rocha D, Lopes CC, Prado da Silva MH, Sinhoreti MAC, Correr-Sobrinho L et al (2021) Effect of pH level and calcination on the production of calcium phosphates by acidic route of wet precipitation. Ceramica 67:236–243. https://doi.org/10.1590/0366-6913202167382296510.1590/0366-69132021673822965 ↗
28. Disha SA, Sahadat Hossain M, Habib ML, Ahmed S (2024) Calculation of crystallite sizes of pure and metals doped hydroxyapatite engaging Scherrer method, Halder-Wagner method, Williamson-Hall model, and size-strain plot. Results Materials 21:100496. https://doi.org/10.1016/j.rinma.2023.10049610.1016/j.rinma.2023.100496 ↗
29. Sabando C, Ide W, Rodríguez-Llamazares S, Bastías RM, Valenzuela M, Rojas C et al (2022) Preparation of poly(vinyl alcohol) microparticles for freeze protection of sensitive fruit crops. Polymers 14:1–12. https://doi.org/10.3390/polym1412245210.3390/polym14122452 ↗
30. Irudhayam V, Veerabathiran T (2021) Synthesis and characterization of PVA assisted Sr-hydroxyapatite using hydrothermal coupled microemulsion method. Res Sq 1–1410.21203/rs.3.rs-576758/v1 ↗
31. Liu Z, Lanier OL, Chauhan A (2020) Poly (vinyl alcohol) assisted synthesis and anti-solvent precipitation of gold nanoparticles. Nanomaterials 10:1–16. https://doi.org/10.3390/nano1012235910.3390/nano10122359 ↗
32. Sheikh L, Tripathy S, Nayar S (2016) Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery. RSC Adv 6:62556–62571. https://doi.org/10.1039/c6ra06759j10.1039/c6ra06759j ↗
33. Quinaz T, Freire TF, Olmos A, Martins M, Ferreira FBN, de Moura MFSM et al (2024) The influence of hydroxyapatite crystals on the viscoelastic behavior of poly(vinyl alcohol) braid systems. Biomimetics 9:93. https://doi.org/10.3390/biomimetics902009310.3390/biomimetics9020093 ↗
34. Restrepo CV, Villa CC (2021) Synthesis of silver nanoparticles, influence of capping agents, and dependence on size and shape: a review. Environ Nanotechnol Monit Manage 15:100428. https://doi.org/10.1016/j.enmm.2021.10042810.1016/j.enmm.2021.100428 ↗
35. Wen Y, Jinsheng Li HL, Huang H, Song K, Duan K, Guo T, * Weng J (2021) Improvement of drug-loading properties of hydroxyapatite particles using triethylamine as a capping agent: a novel approach. Crystals (Basel) 1–16. https://doi.org/10.3390/cryst1106070310.3390/cryst11060703 ↗
36. Junaidi, Triyana K, Suharyadi E, Harsojo, Wu LY (2017) The roles of polyvinyl alcohol (PVA) as the capping agent on the polyol method for synthesizing silver nanowires. J Nano Res 49:174–80. https://doi.org/10.4028/www.scientific.net/JNanoR.49.17410.4028/www.scientific.net/JNanoR.49.174 ↗
37. Suárez-López R, Puntes VF, Bastús NG, Hervés C, Jaime C (2022) Nucleation and growth of gold nanoparticles in the presence of different surfactants. A dissipative particle dynamics study. Sci Rep. https://doi.org/10.1038/s41598-022-18155-210.1038/s41598-022-18155-2 ↗
38. Morsy SM (2014) Role of surfactants in nanotechnology and their applications. Int J Curr Microbiol App Sci 3(5):237–260
39. Goh KW, Wong YH, Ramesh S, Chandran H, Krishnasamy S, Sidhu A et al (2021) Effect of pH on the properties of eggshell-derived hydroxyapatite bioceramic synthesized by wet chemical method assisted by microwave irradiation. Ceram Int 47:8879–8887. https://doi.org/10.1016/j.ceramint.2020.12.00910.1016/j.ceramint.2020.12.009 ↗
40. Ghazanfar E, Marwat MA, Batool SA, Anwar A, Abdullah SM, Din ZU et al (2024) Electrophoretic deposition of polyvinyl alcohol, C-H NRs along with moringa on an SS substrate for orthopedic implant applications. RSC Adv 14:26775–26787. https://doi.org/10.1039/d4ra03931a10.1039/d4ra03931a ↗
41. Herbanu A, Ana ID, Ardhani R, Siswomihardjo W (2023) Fibrous PVA matrix containing strontium-substituted hydroxyapatite nanoparticles from golden apple snail (Pomacea canaliculata L.) shells for bone tissue engineering. Bioengineering. https://doi.org/10.3390/bioengineering1007084410.3390/bioengineering10070844 ↗
42. Chocholata P, Kulda V, Dvorakova J, Supova M, Zaloudkova M, Babuska V (2021) In situ hydroxyapatite synthesis enhances biocompatibility of PVA/HA hydrogels. Int J Mol Sci. https://doi.org/10.3390/ijms2217933510.3390/ijms22179335 ↗
43. Januariyasa IK, Ana ID, Yusuf Y (2020) Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering. Mater Sci Eng C Mater Biol Appl 107:110347. https://doi.org/10.1016/j.msec.2019.11034710.1016/j.msec.2019.110347 ↗
44. Allender CJ, Bowen JL, Celorrio V, Davies-Jones JA, Davies PR, Guan S et al (2020) The role of growth directors in controlling the morphology of hematite nanorods. Nanoscale Res Lett. https://doi.org/10.1186/s11671-020-03387-w10.1186/s11671-020-03387-w ↗
45. Solovyeva EV, Odintsova OV, Svinko VO, Makeeva DV, Danilov DV (2023) Hydroxyapatite-nanosilver composites with plasmonic properties for application in surface-enhanced raman spectroscopy. Mater Today Commun 35:105908. https://doi.org/10.1016/j.mtcomm.2023.10590810.1016/j.mtcomm.2023.105908 ↗
46. Lara-Ochoa S, Ortega-Lara W, Guerrero-Beltrán CE (2021) Hydroxyapatite nanoparticles in drug delivery: physicochemistry and applications. Pharmaceutics 13:1–24. https://doi.org/10.3390/pharmaceutics1310164210.3390/pharmaceutics13101642 ↗
47. Rodríguez-Lugo V, Karthik TVK, Mendoza-Anaya D, Rubio-Rosas E, Villaseñor Cerón LS, Reyes-Valderrama MI et al (2018) Wet chemical synthesis of nanocrystalline hydroxyapatite flakes: effect of pH and sintering temperature on structural and morphological properties. R Soc Open Sci. https://doi.org/10.1098/rsos.18096210.1098/rsos.180962 ↗
48. Nandiyanto ABD, Oktiani R, Ragadhita R (2019) How to read and interpret FTIR spectroscope of organic material. Indones J Sci Technol 4(1):97–118. https://doi.org/10.17509/ijost.v4i1.1580610.17509/ijost.v4i1.15806 ↗
49. Fadia P, Tyagi S, Bhagat S, Nair A, Panchal P, Dave H et al (2021) Calcium carbonate nano- and microparticles: synthesis methods and biological applications. 3 Biotech. https://doi.org/10.1007/s13205-021-02995-210.1007/s13205-021-02995-2 ↗
50. Yotsova R, Peev S (2024) Biological properties and medical applications of carbonate apatite: a systematic review. Pharmaceutics. https://doi.org/10.3390/pharmaceutics1602029110.3390/pharmaceutics16020291 ↗
51. Pereira MBB, França DB, Araújo RC, Silva Filho EC, Rigaud B, Fonseca MG et al (2020) Amino hydroxyapatite/chitosan hybrids reticulated with glutaraldehyde at different pH values and their use for diclofenac removal. Carbohydr Polym 236:1–41. https://doi.org/10.1016/j.carbpol.2020.11603610.1016/j.carbpol.2020.116036 ↗
52. Djošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V et al (2023) Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. Mater Chem Phys 303:303. https://doi.org/10.1016/j.matchemphys.2023.12776610.1016/j.matchemphys.2023.127766 ↗
53. Fleisher-Craver HC, Vigh G (2008) PVA-based tunable buffering membranes for isoelectric trapping separations. Electrophoresis 29:4247–4256. https://doi.org/10.1002/elps.20080018010.1002/elps.200800180 ↗
54. Javed R, Zia M, Naz S, Aisida SO, Ain N, Ao Q (2020) Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. J Nanobiotechnology. https://doi.org/10.1186/s12951-020-00704-410.1186/s12951-020-00704-4 ↗
55. Rocha RLP, Morais AÍS, Araujo FP, Honório LMC, Silva MP, Furtini MB et al (2025) Enhanced photocatalytic performance of TiO2@Er-hydroxyapatite composite for cationic dye and drug removal. ACS Omega 10:5351–5361. https://doi.org/10.1021/acsomega.4c0631410.1021/acsomega.4c06314 ↗
56. Lv C, Zhang J, Wu L, Ouyang G, Hou X (2023) Turning hydroxyapatite from insulator to visible-light induced photocatalytic membrane through oxygen vacancy introduction and hetero-junction forming with chitosan. Carbohydr Polym. https://doi.org/10.1016/j.carbpol.2022.12023510.1016/j.carbpol.2022.120235 ↗
57. Mondal S, Park S, Choi J, Vu TTH, Doan VHM, Vo TT et al (2023) Hydroxyapatite: a journey from biomaterials to advanced functional materials. Adv Colloid Interface Sci. https://doi.org/10.1016/j.cis.2023.10301310.1016/j.cis.2023.103013 ↗
58. Javed R, Zia M, Naz S, Aisida SO, Ain N, Ao Q (2020) Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. J Nanobiotechnol. https://doi.org/10.1186/s12951-020-00704-410.1186/s12951-020-00704-4 ↗
59. Zhao Y, Wang Y, Ran F, Cui Y, Liu C, Zhao Q et al (2017) A comparison between sphere and rod nanoparticles regarding their in vivo biological behavior and pharmacokinetics. Sci Rep. https://doi.org/10.1038/s41598-017-03834-210.1038/s41598-017-03834-2 ↗
60. Kesharwani P, Ma R, Sang L, Fatima M, Sheikh A, Abourehab MAS et al (2023) Gold nanoparticles and gold nanorods in the landscape of cancer therapy. Mol Cancer. https://doi.org/10.1186/s12943-023-01798-810.1186/s12943-023-01798-8 ↗
61. Thamizhchelvan AM, Ma H, Wu T, Nguyen D, Padelford J, Whitworth TJ et al (2024) Shape-dependent cellular uptake of iron oxide nanorods: mechanisms of endocytosis and implications on cell labeling and cellular delivery. Nanoscale 16:21398–21415. https://doi.org/10.1039/d4nr02408g10.1039/d4nr02408g ↗
62. Abdelfatah M, Darwesh N, Habib MA, Alduaij OK, El-Shaer A, Ismail W (2013) Enhancement of structural, optical and photoelectrochemical properties of n−Cu2O thin films with K ions doping toward biosensor and solar cell applications 13. https://doi.org/10.3390/xxxxx10.3390/xxxxx ↗
63. Bera B, Chakraborty A, Kar T, Leuaa P, Neergat M (2017) Density of states, carrier concentration, and flat band potential derived from electrochemical impedance measurements of N-doped carbon and their influence on electrocatalysis of oxygen reduction reaction. J Phys Chem C Nanomater Interfaces 121:20850–20856. https://doi.org/10.1021/acs.jpcc.7b0673510.1021/acs.jpcc.7b06735 ↗
64. Sundarabharathi L, Ponnamma D, Parangusan H, Chinnaswamy M, Al-Maadeed MAA (2020) Effect of anions on the structural, morphological and dielectric properties of hydrothermally synthesized hydroxyapatite nanoparticles. SN Appl Sci. https://doi.org/10.1007/s42452-019-1807-310.1007/s42452-019-1807-3 ↗
65. Arab M, Behboodi P, Malek Khachatourian A, Nemati A (2024) Enhanced mechanical properties and biocompatibility of hydroxyapatite scaffolds by magnesium and titanium oxides for bone tissue applications. Heliyon. https://doi.org/10.1016/j.heliyon.2024.e3384710.1016/j.heliyon.2024.e33847 ↗
66. Chen YC, Chen YJ, Dong PH, Hsu YK (2020) Benchmarked photoelectrochemical water splitting by nickel-doped n-type cuprous oxide. ACS Appl Energy Mater 3:1373–1380. https://doi.org/10.1021/acsaem.9b0178110.1021/acsaem.9b01781 ↗
67. Kalubowila KDRN, Jayathileka KMDC, Kumara LSR, Ohara K, Kohara S, Sakata O et al (2019) Effect of bath pH on electronic and morphological properties of electrodeposited Cu2O thin films. J Electrochem Soc 166:D113–D119. https://doi.org/10.1149/2.0551904jes10.1149/2.0551904jes ↗
68. Liang X, He Q, Zhang J, Ding X, Gao Y, Chen W et al (2022) Enhanced photo-carrier transportation at semiconductor/electrolyte interface of TiO2 photoanode by oxygen vacancy engineering. Appl Surf Sci. https://doi.org/10.1016/j.apsusc.2022.15374410.1016/j.apsusc.2022.153744 ↗
69. Kavan L (2024) Electrochemistry and band structure of semiconductors (TiO2, SnO2, ZnO): avoiding pitfalls and textbook errors. J Solid State Electrochem 28:829–845. https://doi.org/10.1007/s10008-023-05770-w10.1007/s10008-023-05770-w ↗
70. Gelderman K, Lee L, Donne SW (2007) Flat-band potential of a semiconductor: using the Mott-Schottky equation. J Chem Educ 84(4):68510.1021/ed084p685 ↗
71. Li F, Yue X, Liao Y, Qiao L, Lv K, Xiang Q (2023) Understanding the unique S-scheme charge migration in triazine/heptazine crystalline carbon nitride homojunction. Nat Commun. https://doi.org/10.1038/s41467-023-39578-z10.1038/s41467-023-39578-z ↗
72. Wayman TMR, Lomonosov V, Ringe E (2024) Capping agents enable well-dispersed and colloidally stable metallic magnesium nanoparticles. J Phys Chem C Nanomater Interfaces 128:4666–4676. https://doi.org/10.1021/acs.jpcc.4c0036610.1021/acs.jpcc.4c00366 ↗
73. Alorku K, Manoj M, Yuan A (2020) A plant-mediated synthesis of nanostructured hydroxyapatite for biomedical applications: a review. RSC Adv 10:40923–40939. https://doi.org/10.1039/d0ra08529d10.1039/d0ra08529d ↗
74. Zeb A, Saliman M, Wajid A, Ullah H, Zia T ul H (2024) Cathodic shift in flatband potential of hematite based photoelectrodes: evaluating and correlating the redshift to solar driven photocatalysis. Desalin Water Treat 320:100792. https://doi.org/10.1016/j.dwt.2024.10079210.1016/j.dwt.2024.100792 ↗
75. Hamdani IR, Bhaskarwar AN (2022) Tuning of the structural, morphological, optoelectronic and interfacial properties of electrodeposited Cu2O towards solar water-splitting by varying the deposition pH. Sol Energy Mater Sol Cells. https://doi.org/10.1016/j.solmat.2022.11171910.1016/j.solmat.2022.111719 ↗
76. Koiki BA, Orimolade BO, Zwane BN, Nkosi D, Mabuba N, Arotiba OA (2020) Cu2O on anodised TiO2 nanotube arrays: a heterojunction photoanode for visible light assisted electrochemical degradation of pharmaceuticals in water. Electrochim Acta 340:340. https://doi.org/10.1016/j.electacta.2020.13594410.1016/j.electacta.2020.135944 ↗
77. Soni J, Koser AA (2015) Effect of capping agent concentration on ZnO nanoparticles 12
78. Rodrigues A, Sena da Fonseca B, Ferreira Pinto AP, Piçarra S, Montemor MF (2022) Synthesis and application of hydroxyapatite nanorods for improving properties of stone consolidants. Ceram Int 48:14606–14617. https://doi.org/10.1016/j.ceramint.2022.02.00410.1016/j.ceramint.2022.02.004 ↗
79. van der Heijden O, Park S, Vos RE, Eggebeen JJJ, Koper MTM (2024) Tafel slope plot as a tool to analyze electrocatalytic reactions. ACS Energy Lett 9:1871–1879. https://doi.org/10.1021/acsenergylett.4c0026610.1021/acsenergylett.4c00266 ↗
80. Bartoli F, Castello C, Peruzzolo T, Berretti E, Sediva E, Bouzek K et al (2025) MoO3-xNiMoO4 nanorods synthesized using NiO nanoparticles for hydrogen evolution in anion exchange membrane water electrolysis. Chem Eng J. https://doi.org/10.1016/j.cej.2025.16202610.1016/j.cej.2025.162026 ↗
81. Matvieiev O, Šelešovská R, Marton M, Hatala M, Metelka R, Weis M et al (2023) Effect of different modification by gold nanoparticles on the electrochemical performance of screen-printed sensors with boron-doped diamond electrode. Sci Rep. https://doi.org/10.1038/s41598-023-48834-710.1038/s41598-023-48834-7 ↗
82. Meng L, Shu M, Mei P, Liang Y, Xia L (2025) Size-controllable synthesis of hydroxyapatite nanorods via fluorine modulation: applications in dental adhesives for enhanced enamel remineralization. BMC Oral Health. https://doi.org/10.1186/s12903-025-05574-610.1186/s12903-025-05574-6 ↗
83. da Silva DM, de Menezes BRC, Bezzon VDN, do Amaral Montanheiro TL, de Macedo EF, Tada DB et al (2019) Phase transition of TiO2 nanoparticles into titanate nanorods via hydrothermal reactions. SN Appl Sci 1. https://doi.org/10.1007/s42452-019-1004-410.1007/s42452-019-1004-4 ↗
84. Liu T, Li M, Wang Y, Fang Y, Wang W (2018) Electrochemical impedance spectroscopy of single Au nanorods. Chem Sci 9:4424–4429. https://doi.org/10.1039/c8sc00983j10.1039/c8sc00983j ↗
85. Goloshchapov D, Seredin P, Minakov D, Domashevskaya E (2018) Study of the nanoporous CHAP photoluminiscence for developing the precise methods of early caries detection. IOP Conf Ser Mater Sci Eng. https://doi.org/10.1088/1757-899X/307/1/01202710.1088/1757-899X/307/1/012027 ↗
86. Van HN, Tam PD, Kien NDT, Huy PT, Pham VH (2017) Enhancing the luminescence of Eu3+/Eu2+ ion-doped hydroxyapatite by fluoridation and thermal annealing. Luminescence 32:817–823. https://doi.org/10.1002/bio.325710.1002/bio.3257 ↗
87. Machado TR, Sczancoski JC, Beltrán-Mir H, Nogueira IC, Li MS, Andrés J et al (2017) A novel approach to obtain highly intense self-activated photoluminescence emissions in hydroxyapatite nanoparticles. J Solid State Chem 249:64–69. https://doi.org/10.1016/j.jssc.2016.12.01810.1016/j.jssc.2016.12.018 ↗
88. Su W, Hu Y, Zeng M, Li M, Lin S, Zhou Y et al (2019) Design and evaluation of nano-hydroxyapatite/poly(vinyl alcohol) hydrogels coated with poly(lactic-co-glycolic acid)/nano-hydroxyapatite/poly(vinyl alcohol) scaffolds for cartilage repair. J Orthop Surg Res 14:1–9. https://doi.org/10.1186/s13018-019-1450-010.1186/s13018-019-1450-0 ↗
89. Junnarkar MV, Sawant PV, Parekar MA, Kardile AV, Thorat AB, Joshi RP et al (2024) Silver-blend hydroxyapatite bio-ceramics for enhanced photocatalytic degradation of methylene blue. Academia Materials Science. https://doi.org/10.20935/acadmatsci624010.20935/acadmatsci6240 ↗
90. Radecka M, Rekas M, Trenczek-Zajac A, Zakrzewska K (2008) Importance of the band gap energy and flat band potential for application of modified TiO2 photoanodes in water photolysis. J Power Sources 181:46–55. https://doi.org/10.1016/j.jpowsour.2007.10.08210.1016/j.jpowsour.2007.10.082 ↗
91. Sun H, Jiang C, Wu L, Bai X, Zhai S (2019) Cytotoxicity-related bioeffects induced by nanoparticles: the role of surface chemistry. Front Bioeng Biotechnol. https://doi.org/10.3389/fbioe.2019.0041410.3389/fbioe.2019.00414 ↗
92. Liu T, Yang F, Wang L, Pei L, Hu Y, Li R et al (2022) Synergistic effect of charge separation and multiple reactive oxygen species generation on boosting photocatalytic degradation of fluvastatin by ZnIn2S4/Bi2WO6 z-scheme heterostructured photocatalytst. Toxics. https://doi.org/10.3390/toxics1010055510.3390/toxics10100555 ↗
93. Elbasuney S, El-Khawaga AM, Elsayed MA, Elsaidy A, Correa-Duarte MA (2023) Enhanced photocatalytic and antibacterial activities of novel Ag-HA bioceramic nanocatalyst for waste-water treatment. Sci Rep. https://doi.org/10.1038/s41598-023-40970-410.1038/s41598-023-40970-4 ↗
94. Mondal S, De Anda Reyes ME, Pal U (2017) Plasmon induced enhanced photocatalytic activity of gold loaded hydroxyapatite nanoparticles for methylene blue degradation under visible light. RSC Adv 7:8633–8645. https://doi.org/10.1039/C6RA28640B10.1039/C6RA28640B ↗
95. He Z, Sun S, Deng C (2020) Effect of hydroxyapatite coating surface morphology on adsorption behavior of differently charged proteins. J Bionic Eng 17:345–356. https://doi.org/10.1007/s42235-020-0028-110.1007/s42235-020-0028-1 ↗
96. Sun W, Zhong J, Gao B, Feng J, Ye Z, Lin Y et al (2024) In vitro/in vivo evaluations of hydroxyapatite nanoparticles with different geometry. Int J Nanomedicine 19:8661–8679. https://doi.org/10.2147/IJN.S46968710.2147/IJN.S469687 ↗
97. Liang W, Ding P, Li G, Lu E, Zhao Z (2021) Hydroxyapatite nanoparticles facilitate osteoblast differentiation and bone formation within sagittal suture during expansion in rats. Drug Des Devel Ther 15:905–917. https://doi.org/10.2147/DDDT.S29964110.2147/DDDT.S299641 ↗
98. Carles-Carner M, Saleh LS, Bryant SJ (2018) The effects of hydroxyapatite nanoparticles embedded in a MMP-sensitive photoclickable PEG hydrogel on encapsulated MC3T3-E1 pre-osteoblasts. Biomed Mater. https://doi.org/10.1088/1748-605X/aabb3110.1088/1748-605X/aabb31 ↗
99. Jin Y, Liu X, Liu H, Chen S, Gao C, Ge K et al (2017) Oxidative stress-induced apoptosis of osteoblastic MC3T3-E1 cells by hydroxyapatite nanoparticles through lysosomal and mitochondrial pathways. RSC Adv 7:13010–13018. https://doi.org/10.1039/c7ra01008g10.1039/c7ra01008g ↗
100. Parayanthala Valappil M, Santhakumar S, Arumugam S (2014) Determination of oxidative stress related toxicity on repeated dermal exposure of hydroxyapatite nanoparticles in rats. Int J Biomater 2014:476942. https://doi.org/10.1155/2014/47694210.1155/2014/476942 ↗