Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions
Source abstract
Abstract
The effect of manufactured gold nanoparticles (NP) on the immune system was analysed through their ability to perturb the functions of dendritic cells (DC), a major actor of both innate and acquired immune responses. For this purpose, DCs were produced in culture from mouse bone marrow progenitors.The analysis of the viability of the cells after their incubation in the presence of gold NP shows that these NP are not cytotoxics even at high concentration. Furthermore, the phenotype of the DC is unchanged after the addition of NP, indicating that there is no activation of the DC. But the analysis of the cells at the intracellular level reveals important amounts of gold NP amassing in endocytic compartments. Furthermore, the secretion of cytokines is significantly modified after such internalisation indicating a potential perturbation of the immune response.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Immunotherapy and Immune Responses · Nanoparticles: synthesis and applications · Reproductive System and Pregnancy
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (10)
Show source-provided references
1. Bueno C, Almeida J, Alguero MC et al (2001) Flow cytometric analysis of cytokine production by normal human peripheral blood dendritic cells and monocytes: comparative analysis of different stimuli, secretion-blocking agents and incubation periods. Cytometry 46:33–4010.1002/1097-0320(20010215)46:1<33::AID-CYTO1035>3.0.CO;2-S ↗
2. Chou CC, Hsiao HY, Hong QS et al (2008) Single-walled carbon nanotubes can induce pulmonary injury in mouse model. Nano Lett 8:437–44510.1021/nl0723634 ↗
3. Elamanchili P, Diwan M, Cao M, Samuel J (2004) Characterization of poly(d,l-lactic-co-glycolic acid) based nanoparticulate system for enhanced delivery of antigens to dendritic cells. Vaccine 22:2406–241210.1016/j.vaccine.2003.12.032 ↗
4. Faure M, Villiers CL, Marche PN (2004) Normal differentiation and functions of mouse dendritic cells derived from RAG-deficient bone marrow progenitors. Cell Immunol 228:8–1410.1016/j.cellimm.2004.04.002 ↗
5. Mitchell LA, Gao J, Wal RV, Gigliotti A, Burchiel SW, McDonald JD (2007) Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. Toxicol Sci 100:203–21410.1093/toxsci/kfm196 ↗
6. Ryan JJ, Bateman HR, Stover A et al (2007) Fullerene nanomaterials inhibit the allergic response. J Immunol 179:665–67210.4049/jimmunol.179.1.665 ↗
7. Savina A, Amigorena S (2007) Phagocytosis and antigen presentation in dendritic cells. Immunol Rev 219:143–15610.1111/j.1600-065X.2007.00552.x ↗
8. Schneider T, Becker A, Ringe K, Reinhold A, Firsching R, Sabel BA (2008) Brain tumor therapy by combined vaccination and antisense oligonucleotide delivery with nanoparticles. J Neuroimmunol 195:21–2710.1016/j.jneuroim.2007.12.005 ↗
9. Shvedova AA, Fabisiak JP, Kisin ER et al (2008) Sequential exposure to carbon nanotubes and bacteria enhances pulmonary inflammation and infectivity. Am J Respir Cell Mol Biol 38:579–59010.1165/rcmb.2007-0255OC ↗
10. Warheit DB, Sayes CM, Reed KL, Swain KA (2008) Health effects related to nanoparticle exposures: environmental, health and safety considerations for assessing hazards and risks. Pharmacol Ther 120:35–4210.1016/j.pharmthera.2008.07.001 ↗