Increasing Antigenecity of B16 Melanoma Cell Fraction with Microwave Hyperthermia

An injection of a fraction of a fraction (C30 mw) obtained by centrifugation at 30 kg of homogenate of B16 melanoma cells, treated with microwave hyperthermia (2450 MHz, CW, 44 degrees C, for 20 minutes), increases the survival time (p less than 0.01) of C57-BL/6J male mice inoculated 26 days later with 10(7) cells (viability greater than 95%) of B16 melanoma. This delay of 26 days between these two injections corresponds to the moment where the synthesis of immunoglobulins is increased in protected mice. The earliest death (p less than 0.001) of animals injected with untreated tumoral cell fraction (C30), and that received a suspension of viable B16 melanoma cells (10(7)) can be explained by an inhibition of humoral immunity system.