Facile construction of beads-on-a-string complexes of polymeric micelles and DNA via tunable electrostatic interactions
Source abstract
Abstract
Abstract Inspired by the structure of chromatin fibers, the compaction of DNA by cationic nanoparticles has received much attention in the fields of nanomaterials and biotechnology. However, the fabrication of artificial chromatin-biomimetic assemblies of DNA and nanoparticles in biological environments is still a challenge due to their dependence on uncontrollable electrostatic interactions. Herein, block and random copolymerization of 2-(diisopropylamino)ethyl methacrylate (DPA) and 2-(dimethylamino)ethyl methacrylate (DMA) were used to regulate the charge density and distribution of cationic polyethylene glycol-b-poly(tertiary amine methacrylate) micelles, adjusting the electrostatic interaction between the micelles and DNA. We showed that the assembled PEG-b-PDMA-b-PDPA micelle/DNA system exhibited a strong electrostatic interactions between the micelles and DNA, causing uncontrollable intrachain flocculation. However, the assembled PEG-b-P(DMA-r-DPA) micelle/DNA system showed relatively week, tunable electrostatic interactions, leading to an open -chromatin-like structure
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · DNA and Nucleic Acid Chemistry · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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