Amino Acid Adsorption Onto Magnetic Nanoparticles Reveals Correlations With Physicochemical Parameters

Abstract We analyze the adsorption of the proteinogenic amino acids (AAs) glutamine, glutamic acid, lysine, tyrosine, proline, and valine onto bare iron oxide nanoparticles (approx. 10 nm). Aiming to identify the governing principles of low molecular weight coronae, which remain underinvestigated, our study covers broad concentration ranges up to the solubility limit of the AAs. Isothermal experiments reveal that the highly soluble AAs valine, proline, and lysine form extensive multilayers on the nanoparticle surface, and infrared measurements indicate intermolecular interactions, particularly with valine and lysine, for higher AA contents. Conversely, the low solubility of tyrosine and glutamic acid restricts their adsorption capacity, despite their higher partitioning on the solid surface. Parameters derived from fitting a classic saturation model seem to align with well‐documented physicochemical properties such as the hydrophobicity and the complexity indices – a promising first step towards formulating design principles. Scaling these parameters by the AA solubility reveals a clear correlation with the adsorption behavior. In adsorption experiments with AA model mixtures, sequential incubation increases the adsorption capacity for valine and proline, whereas simultaneous incubation with these AAs reduces tyrosine's capacity. Future studies should seek to elucidate adsorption patterns to advance our understanding of corona growth and evolution mechanisms.