Dendritic sugar-microarrays by click chemistry

Abstract Saccharide microarrays with well-defined multivalency were prepared using glycodendrimers. The glycodendrimers with various generations and saccharides (α-Man, β-GlcNAc and β-Gal) were synthesized and immobilized on an acetylenyl-terminated gold substrate via click chemistry. The saccharide-immobilized substrates were analyzed using XPS, ellipsometry, cyclic voltammetry, contact angle goniometry, QCM and FTIR-RAS. The biological abilities of the saccharide-immobilized substrates were evaluated by SPR with the lectins of Con A, WGA and RCA 120 . Lectin binding to the glycodendrimer array was quantitatively analyzed, and association constants ( K A ) and kinetic rate constants ( k a and k d ) were evaluated. These kinetic constants were unique to each lectin, implying specificity of the multivalent effect of saccharide–lectin interaction.