Advancing DNA and RNA Modification Detection via Nanopore Sequencing
Source abstract
Abstract
DNA/RNA modifications are crucial for biological processes. To understand their regulatory mechanisms, precise mapping and quantification of these modifications are essential. Although next-generation sequencing can detect the location and stoichiometry of modifications, complex treatments are required, hindering efficiency and long-range analysis. In contrast, nanopore sequencing eliminates the need for additional treatment and PCR amplification, thus preserving DNA/RNA modification information. It enables modifications to be identified and quantified directly at the single-molecule level. Therefore, nanopore sequencing facilitates long-read, real-time modification detection, and has gained widespread applications. This perspective introduces the principles of nanopore sequencing, evaluates its strengths/weaknesses, and critically examines its broader real-world applications. To expand the utility of nanopore sequencing further, we discuss the current challenges and suggest future directions.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: RNA modifications and cancer · Advanced biosensing and bioanalysis techniques · Nanopore and Nanochannel Transport Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies
Peking University
NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, and Department of Obstetrics and Gynecology
The First Affiliated Hospital of Anhui Medical University
State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences
Department of Chemical Biology and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering
Funding
National Key Research and Development Program of China · National Natural Science Foundation of China · Central Guidance for local Science and Technology Development Fund Project
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