journal article·2025-07-29

Establishment of a Cochlear Organoid Platform for Remodeling the Extracellular Matrix

Nianci Li, Ziyu Zhang, Liyan Zhang, Danqi Wu, Zhe Li, Changling Xu, Qiuhan Sun, Yicheng Lu, Yinyi Zhou, Xiaoyun Qian, Yangnan Hu, Fangzhi Tan, Jieyu Qi, Renjie Chai

ACS Nano· Volume 19· Issue 29
Publisher page

Abstract

Irreparable injury and depletion of inner ear hair cells lead to permanent hearing impairment. Cochlear organoids serve as a robust in vitro platform for investigating the pathogenesis of deafness and exploring therapeutic approaches. However, the widely used commercial Matrigel in cochlear organoid culture suffers from issues related to consistency and structural stability. Here, we developed a cochlear organoid culture system using synthetic GelMA hydrogel, which promoted the spontaneous clustering and organization of cochlear stem cells, thereby accelerating organoid formation and facilitating the functional maturation of cochlear hair cells. Moreover, we observed that the extracellular matrix (ECM) underwent rapid and extensive remodeling during the early phase of organoid self-organization. During cochlear organoid development, matrix metalloproteinases (Mmps) were markedly upregulated, with Mmp9 displaying relatively higher expression. Notably, inhibition of Mmps significantly interfered with the organoid formation process, suggesting their important role in cochlear organoid morphogenesis. Collectively, this work provides an alternative approach for cochlear organoid generation and underscores the contribution of Mmp-mediated ECM remodeling during organoid formation.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Hearing, Cochlea, Tinnitus, Genetics · Planarian Biology and Electrostimulation · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, State Key Laboratory of Digital Medical Engineering, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health

Southeast University

Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, School of Medicine

University of Electronic Science and Technology of China

Department of Otolaryngology-Head and Neck Surgery

the Affiliated Drum Tower Hospital of Nanjing University Medical School. Jiangsu Provincial Key Medical Discipline (Laboratory)

Co-Innovation Center of Neuroregeneration

Nantong University

Advanced Technology Research Institute

Beijing Institute of Technology

Southeast University Shenzhen Research Institute

Funding

Taishan Scholar Foundation of Shandong Province · 2022 Open Project Fund of the Guangdong Academy of Medical Sciences grant · Shenzhen Science and Technology Program grant · National Key R&D Program of China grant · Natural Science Foundation of Jiangsu Province grant · China Postdoctoral Science Foundation grant · Shandong Province Outstanding Youth Science Foundation · Beijing Natural Science Foundation · National Natural Science Foundation of China grant · Science and Technology Department of Sichuan Province grant · Jiangsu Provincial Scientific Research Center of Applied Mathematics under Grant · Research Personnel Cultivation Programme of Zhongda Hospital Southeast University grant · STI2030-Major Projects grant · Key Program of Jiangsu Natural Science Foundation

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