Self-Assembly of DNA Nanoflares in Mitochondria for Sensitive In Situ Imaging of Cancer Markers
Source abstract
Abstract
Real-time monitoring of cancer markers at the organelle level offers an effective approach to understanding the mechanisms of cancer generation. Herein, we have developed a sensitive in situ imaging method for human apurinic/apyrimidinic endonuclease 1 (APE1), a cancer biomarker that regulates mitochondrial function by triggering a chain reaction within mitochondria to form highly efficient DNA nanoflares through palindrome-driven assembly (PDA). To deliver the DNA sequences with palindromes for the chain reaction, we prepared triphenylphosphine (TPP)-modified polymeric nanoparticles specifically targeting mitochondria. The presence of APE1 in mitochondria triggered the PDA, leading to the in situ assembly of DNA nanoflares and yielding highly sensitive imaging of APE1. This strategy demonstrates the high capability of combined chain reactions for the real-time monitoring of cancer markers within organelles.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · DNA and Nucleic Acid Chemistry · Biosensors and Analytical Detection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences
Southwest University
Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Chemistry and Chemical Engineering
Funding
Chongqing Municipal Science and Technology Bureau · National Natural Science Foundation of China
References (40)
Show source-provided references
1. 10.1016/j.devcel.2021.03.01210.1016/j.devcel.2021.03.012 ↗
2. 10.1038/s41586-023-06537-z10.1038/s41586-023-06537-z ↗
3. 10.1016/S1474-4422(12)70238-710.1016/S1474-4422(12)70238-7 ↗
4. 10.1038/s41421-020-00233-210.1038/s41421-020-00233-2 ↗
5. 10.1016/j.cell.2016.07.00210.1016/j.cell.2016.07.002 ↗
6. 10.1038/s41586-023-05793-310.1038/s41586-023-05793-3 ↗
7. 10.1038/s41586-023-06857-010.1038/s41586-023-06857-0 ↗
8. 10.1007/s10495-022-01709-010.1007/s10495-022-01709-0 ↗
9. 10.7150/ijbs.8160910.7150/ijbs.81609 ↗
10. 10.1126/science.abe997710.1126/science.abe9977 ↗
11. 10.1016/j.mtnano.2021.10014910.1016/j.mtnano.2021.100149 ↗
12. 10.1016/j.cell.2015.10.00110.1016/j.cell.2015.10.001 ↗
13. 10.1016/j.mito.2013.10.00710.1016/j.mito.2013.10.007 ↗
14. 10.1111/j.1349-7006.2012.02239.x10.1111/j.1349-7006.2012.02239.x ↗
15. 10.1016/j.jphotobiol.2020.11199210.1016/j.jphotobiol.2020.111992 ↗
16. Robertson K. A. · Cancer Res. · 2001 · 61 · 2220
17. 10.1002/anie.20220323810.1002/anie.202203238 ↗
18. 10.1016/j.bios.2022.11436010.1016/j.bios.2022.114360 ↗
19. 10.1021/acs.analchem.3c0257410.1021/acs.analchem.3c02574 ↗
20. 10.1002/anie.20201673810.1002/anie.202016738 ↗
21. 10.1002/anie.20220427710.1002/anie.202204277 ↗
22. 10.1021/acs.chemrev.3c0002810.1021/acs.chemrev.3c00028 ↗
23. 10.1021/jacs.3c0208310.1021/jacs.3c02083 ↗
24. 10.1021/acs.nanolett.2c0293410.1021/acs.nanolett.2c02934 ↗
25. 10.1021/acsnano.9b0999510.1021/acsnano.9b09995 ↗
26. 10.1038/s41467-023-38949-w10.1038/s41467-023-38949-w ↗
27. 10.1002/smtd.20220171210.1002/smtd.202201712 ↗
28. 10.1038/s41467-021-23850-110.1038/s41467-021-23850-1 ↗
29. 10.1038/nnano.2015.24310.1038/nnano.2015.243 ↗
30. 10.1038/nnano.2016.5410.1038/nnano.2016.54 ↗
31. 10.1126/sciadv.abf900010.1126/sciadv.abf9000 ↗
32. 10.1021/jacs.2c1108110.1021/jacs.2c11081 ↗
33. 10.1021/acs.analchem.3c0111710.1021/acs.analchem.3c01117 ↗
34. 10.1021/acs.analchem.2c0094110.1021/acs.analchem.2c00941 ↗
35. 10.1002/smll.20220796110.1002/smll.202207961 ↗
36. 10.1002/anie.20120498910.1002/anie.201204989 ↗
37. 10.1073/pnas.121009610910.1073/pnas.1210096109 ↗
38. 10.1021/acsnano.3c0344310.1021/acsnano.3c03443 ↗
39. 10.1016/j.nantod.2022.10157310.1016/j.nantod.2022.101573 ↗
40. 10.1016/j.lungcan.2009.02.01910.1016/j.lungcan.2009.02.019 ↗