Research on novel polylactic acid / polyglycolic acid nano scaffold loaded drug system

poly-( D,L-lactide-co-glycolide)( PLGA) was biodegradable and biocompatible,and the degradation products of PLGA were non-toxic as raw materials. The PLGA loaded Tetracycline hydrochloride( Tet) nanofibers membrane was fabricated via electrospinning, to prepare tissue engineering scaffolds. The morphology were observed by means of scan electric microscope( SEM) and fluorescence microscopy. Cells and scaffold of the morphology,adhesion and growth were observed by SEM. The absorbency of drug was detected by UV-visible spectroscopy,then counting the velocity of drug release. The antimicrobial activity in vitro were examined by improved Kirby-Bauer diffusion method. The average diameter of PLGA nanofibers with different Tet contents between 360 nm and470 nm. The 10% drug loaded membranes exhibited the highest initial burst release. Tet release sustained over 27 days in vitro. The antimicrobial activity in vitro was effective in inhibiting Staphylococcus aureus growth. Examination of the biocompatibility of PLGA/ Tet scaffolds with MG-63 cells showed no adverse effect on the cell adhesion and proliferation. So PLGA / Tet nanofibers has good biocompatibility.