Immobilization of lysozyme on wool fabric by sol-gel method

Lysozymes were immobilized on wool fabrics by sol-gel method in order to enhance the antibacterial function of wool.The process of immobilization was studied and the optimum parameters were determined,i.e.,propyltrimethoxysilane(PTMS)/tetramethoxysilane(TMOS) molar ratio was 4∶1,water/silicon source substance volume ratio was 32,lysozyme mass concentration was 25 mg/mL,and PVA 0588 mass concentration was 2 mg/mL.Lysozymes immobilized on wool fabrics presented better thermostability and operation stability compared with free lysozymes.The immobilized lysozyme remained 96% of the original enzyme activity after an incubation at 40℃for 100 min,and 52.3% of the original enzyme activity after seven continuous reuses.