Proliferation inhibition effects of ethoxylated fatty alcohol on cells

In view of the safety problem of chemicals for textiles,MTT assay was adopted to study the effects of three different kinds of ethoxylated fatty alcohols on the proliferation inhibition of Chinese hamster ovary(CHO)cells and human cervical carcinoma HeLa cells.The results indicated that the proliferation inhibition effect of ethoxylated fatty alcohol on both CHO cells and HeLa cells gradually decreased as the number of EO increased.In other words,with respect to the proliferation inhibition on both cells,it showed the following order: AEO5 AEO9 AEO15.When the concentrations of AEO5 and AEO9 were higher than 0.050 μL/mL and 0.075 μL/mL,respectively,the increase of proliferation inhibition effect on the cells exhibited insignificant with the increase of the concentration.However,when the concentration of AEO15 was higher than 0.100 μL/mL,its proliferation inhibition effect on the cells still showed an upward tend.When the concentrations of AEO5,AEO9 and AEO15 were lower than 0.005,0.005 and 0.010 μL/mL,respectively,their proliferation inhibition effect on CHO cells and HeLa cells was not noticeable.