Isolation and identification of actinomycete strain DM182 for microbial degumming of hemp

Whether the degumming strains exist in soil sample collected from the roots of hemp was explored in this study.The actinomycetes were cultivated on GAUZE's Medium No.1.The degumming strains of hemp were screened by the method of transparent zones.The strain was classified by physiological,biochemical and morphological characteristics and 16 S rRNA gene sequence analysis.The results showed that a total of 41 actinomyctes were isolated from the samples,of which the strain DM182 was much effective in decomposing the pectin of hemp,and the transparent circle diameter is 3.78 cm.According to its morphological,physiological and biochemical characteristics and 16 S rDNA sequence analysis,the strain DM182 was preliminarily identified as Nocardioides albus.Thus,some members of this genus have a very wide application prospect in agriculture and industry.It is for the first time to report that N.albus strain was isolated in the local region,and the study provided a basis for the further exploration of the strain.