Mutagenesis and screening tests of strains for high-efficiency bio-degumming of ramie

The soil of ramie field and sludge from retting pool were used as the sources of strains for screening,and eight effective strains were obtained from plate screening firstly,then ramie microbial fermentation experiment was performed and the strain which enjoys advantages was screened out.It was affirmed that CJ2 was Bacillus licheniformis after the colony morphology、 physiological and biochemical indexes inspection.The degumming loss percentage of ramie by CJ2 was 17.6%.The mutant B2 was gained from the strain CJ2 by UV mutagenesis for 60 s,whose degumming loss percentage was 22.8%,29.5% higher than that of the strain CJ2.The results showed that it was effective to use UV mutagenesis for screening high-efficieny strain for biological degumming of ramie,and the strain B2 was a suitable choice as the receptor bacteria in the microbial degumming of ramie.