DataVXACS NanoPaper
journal article·2024-08-27Open access

Charge Detection Mass Spectrometry Enables Molecular Characterization of Nucleic Acid Nanoparticles

Polycarp C. Ofoegbu, Grant A. Knappe, Anna Romanov, Benjamin E. Draper, Mark Bathe, Martin F. Jarrold

ACS Nano· Volume 18· Issue 34
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Source abstract

Abstract

Nucleic acid nanoparticles (NANPs) are increasingly used in preclinical investigations as delivery vectors. Tools that can characterize assembly and assess quality will accelerate their development and clinical translation. Standard techniques used to characterize NANPs, like gel electrophoresis, lack the resolution for precise characterization. Here, we introduce the use of charge detection mass spectrometry (CD-MS) to characterize these materials. Using this technique, we determined the mass of NANPs varying in size, shape, and molecular mass, NANPs varying in production quality due to formulations lacking component oligonucleotides, and NANPs functionalized with protein and nucleic acid-based secondary molecules. Based on these demonstrations, CD-MS is a promising tool to precisely characterize NANPs, enabling more precise assessments of the manufacturing and processing of these materials.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, Indiana University, Bloomington, Indiana 47405, United States

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

Megadalton Solutions Inc, 3750 E Bluebird Ln, Bloomington, Indiana 47401, United States

Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02139, United States

Harvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, Massachusetts 02115, United States

Funding

National Science Foundation Graduate Research Fellowship Program · Army Research Office · National Institutes of Health

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