Comparative study of proliferation inhibiting effect of two kinds of surfactants on cells

MTT assay was adopted to study the proliferation inhibiting effect of two kinds of N,N-dialkyl urea compounds and four kinds of alkylphenol ethoxylates surfactants on HeLa cells and CHO cells,and the cytotoxicity of the two kinds of non-ionic surfactants were discussed.The results indicated that the proliferation inhibiting effect of N,N-dialkyl urea and alkylphenol ethoxylates on both HeLa cells and CHO cells gradually increased as the concentration increased.N,N-diethyl urea and N,N-dipropyl urea could inhibit the proliferation of HeLa cells and CHO cells at the concentration of 10 mg/mL,and the inhibition rate was more than 80%.Alkylphenol ethoxylates OP-7,OP-9,NP-10 and NP-25 could inhibit the proliferation of HeLa cells and CHO cells at the concentration of 0.1,0.1,0.1,0.5 mg/mL,and the inhibition rate was more than 80%.Therefore,when the six substances reach a certain concentration,they inhibits the proliferation of HeLa cells and CHO cells significantly,and the cytotoxicity of N,N-dialkyl urea compounds is much lower than that of alkylphenol ethoxylates.