Optimization of culture conditions for laccase production from Polyporus elegans Fr.

The 9 factors that influenced laccase production from Polyporus elegans Fr.were studied using Plackett-Burman design,it was found that they were main nutrition factors such as C/N ratio,the concentration of Cu2+ and pH.On that basis,response surface analysis was adopted to investigate the optimal levels of the 3 main factors.The second regression analysis of the findings using Design-Expert software showed that the laccase activity was 446 U/mL(178 U/mL before optimization) when the C/N ratio,concentration of Cu2+ and pH were 4∶1,0.1 mmol/L and 5.5 respectively,which was 2.5 folds of that under the original culture conditions.