Determination of 3 kinds of phenolic environmental hormones in cashmere fabrics by HPLC-DAD-FLD

A method for determination of phenolic environmental hormones such as bisphenol A,4-nonylphenol and octylphenol in cashmere fabrics by high performance liquid chromatography with diode array detector and fluorescence detector(HPLC-DAD-FLD) was established.After cashmere fabrics were extracted by Soxhlet extraction,the extracts were separated and eluted on a fast chromatographic column Shim-pack XR-ODSⅡ by using a mobile phase consisting of CH3OH/CH3CN/H2O(at a volume ratio 30∶50∶20).Detection was performed by DAD at 225 nm and FLD at 228 nm and 305 nm using extenal standard method.The experimental results indicate that calibration curves of 3 kinds of phenolic environmental hormones were linear within the range of 0.01-1.0 mg/kg for FLD,the lower limit of quantification was found to be 0.01 mg/kg.Calibration curves were linear within the range of 0.05-10.0 mg/kg for UV,the lower limit of quantification was 0.05 mg/kg.The extraction recovey was 82.0%-102.0% and the average recovery was 89.2%,the RSD was 1.8%-7.5%.This method is found to be simple,sensitive,accurate and little interference for the determination of bisphenol A,4-n-nonylphenol and octylphenol residue in cashmere fabrics.