Establishment of HPLC separation method for bis(halogeno-s-triazine) reactive dyes and their hydrolysates

In order to study the hydrolysis reaction of bis(halogeno-s-triazine) reactive dyes,the reversed-phase ion-pair high performance liquid chromatography was employed to separate the bis(halogeno-s-triazine) reactive dyes and their hydrolysis products.The establishing procedure of HPLC separation method that is appropriate for bis(halogeno-s-trazine) reactive dyes and their hydrolysis products was discussed in terms of selection of bis(halogeno-s-triazine)reactive dyes and chromatographic column,selection and optimization of mobile phase,determination of column temperature and so on.By this method the bis(monofluo-s-triazine) reactive dye Reactive Red LS-2G and bis(monochloro-s-triazine) reactive dye Reactive Red KE-3B and their hydrolysis products were separated completely.