Chromogenic method determination of 23 kinds of carcinogenic aromatic amines

In order to reduce the dependence on large-scale chromatographs,improve the detection speed and cut the cost of testing,a chromogenic determination method for 23 kinds of carcinogenic aromatic amines through reacting with guaiacol was developed.According to the structural features of aromatic amines and mechanism of diazo-coupling reaction,all terms of diazo-coupling reaction process were studied and optimized effectively.The optimal conditions were obtained as follows: at room temperature,23 kinds of carcinogenic aromatic amines reacted with sodium nitrite and diazo salt was formed at a pH value of 2.56,and then added ammonium sulfamate,removing the excessive sodium nitrite,guaiacol was added to produce coupling /developing reaction with the diazo salt;the best developing pH ranged from 10 to 11,and developing reaction could be completed quickly,and the resulting azo compound remained stable within 180 min.Linearity was excellent in the concentration range of 1-50 mg/L.This method could be effectively applied to textiles in qualitative screening test of 23 kinds of carcinogenic aromatic amines.