Separation of pigments extracted from natural brown cotton fiber by liquid chromatography

Aiming at exploring the components and the specific molecular structure of natural brown cotton pigments,the high-performance liquid chromatography(HPLC) with polyamide as the column was used to separate pigments extracted from natural brown cotton fiber.The results showed that the pigment extract can be successfully fractionated by polyamide column chromatography,and the pigments consist of two fractions for their different colors and HPLC chromatograms of elution segments.Two colored component and one colorless component were separated from water elution segment by C18-reversed-phase chromatography,but the other segment can not be separated by this chromatography column,and according to the retention action on chromatography columns,this part of pigment may belong to a series of polyphenol compounds that have very similar structures.