Process optimization of composite microorganism degumming of ramie

Mixed seed liquid of bacillus B2 and aspergillus M2 was used in the degumming of ramie,and the optimal conditions of joint strains for enzymatic degumming of ramie were determined by the L18(36) orthogonal experiment as follows: bacillus B2 and aspergillus M2 seed liquid inoculation amounts are 6% and 11%,respectively,the ratio of water and material is 15∶1(mL/g),initial pH value is 6,degumming at 35 ℃ for 50 hours.Under these conditions,the degumming rate of the ramie can reach 31.9%,and its fiber fineness and breaking strength meet the standard of the second grade refined ramie.Infrared spectrometry and scanning electron microscopy analysis show that after bio-enzymatic degumming,the smoothness and fineness of the ramie have been improved significantly,and its molecular structure of the glue complex has changed obviously.