Modification of silk fibroin nanofibrous scaffold and its blood compatibility

The electrospun silk fibroin nanofibrous scaffolds were modified with heparin by grafting after plasma treatment and blending electrospinning.The effect of the modification was evaluated by means of SEM and XPS.Platelet adhesion tests and in vitro coagulative time tests were carried out on the silk fibroin nanofibrous scaffolds before and after modification.The results showed that the heparin was successfully introduced to the silk fibroin nanofibrous scaffolds by both two kinds of modification.In vitro coagulative time tests showed that the APTT,PT and TT of the heparin-modified scaffolds were significantly higher than that of the pure silk fibroin scaffolds.Platelet adhesion and aggregation was found on the pure silk fibroin scaffolds.However,it could not be seen on the heparin-modified scaffolds.