Screening and identification of a bacterium strain for specific ramie degumming

When refering to the traditional chemical degumming of the ramie,there are defects such as serious environmental pollution and damage to ramie fibers,but bio-degumming can overcome the drawbacks inherent in chemical degumming.In this paper,a bacterium strain with high-efficient ramie degumming,named Dazu 5-1,was isolated from field soil by iterative enrichment,screening and rescreening.When the strain is used for 2 d fermentation to the ramie degumming,the average weight loss can reach 30.2%.Furthermore,when compared with chemical degumming,Dazu 5-1 strain increases the average value of single fiber strength of the degummed ramie by 76.93%.Dazu 5-1 strain is identified as Shigella by morphological observation,physiological and biochemical indices determination,16S rDNA base sequencing and homology analysis.