DataVXACS NanoPaper
journal article·2025-01-14

Neuronal Tracing and Visualization of Nerve Injury by a Membrane-Anchoring Aggregation-Induced Emission Probe

Rufan Mo, Ying Peng, Zeyang Ding, Huilin Xie, Zijie Qiu, Parvej Alam, Yong Liu, Gang Chen, Jianquan Zhang, Zheng Zhao, Ben Zhong Tang

ACS Nano· Volume 19· Issue 1
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Source abstract

Abstract

Deciphering neuronal circuits is pivotal for deepening our understanding of neuronal functions and advancing treatments for neurological disorders. Conventional neuronal tracers suffer from restrictions such as limited penetration depth, high immunogenicity, and inadequacy for long-term and in vivo imaging. In this context, we introduce an aggregation-induced emission luminogen (AIEgen), MeOTFVP, engineered for enhanced neuronal tracing and imaging. MeOTFVP is strategically designed to target cell membranes by integrating into the phospholipid bilayer through its amphipathy. The donor–acceptor molecular skeleton facilitates a red shift of its photoluminescence into the near-infrared (NIR) spectrum, significantly improving tissue penetration. The affinity of MeOTFVP for cell membranes, coupled with its deep tissue penetration, allows precise tracing in the paw-dorsal root ganglia (DRG) circuit and detailed imaging of the sciatic nerve. This study showcases the application of MeOTFVP as a dual-function neuronal tracer, propelling forward the possibilities for advanced neuronal tracing and imaging using AIEgens.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Neuroscience and Neural Engineering · Luminescence and Fluorescent Materials · Ion channel regulation and function

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Clinical Translational Research Center of Aggregation-Induced Emission, School of Medicine, The Second Affiliated Hospital, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), Shenzhen 518172, P. R. China

Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Kowloon, Hong Kong 999077, P. R. China

AIE Institute, Guangzhou, Guangdong Province 510530, China

School of Medicine, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), Shenzhen 518172, Guangdong, China

Funding

National Key Research and Development Program of China · Science, Technology and Innovation Commission of Shenzhen Municipality · Innovation and Technology Commission · National Natural Science Foundation of China · Shenzhen Key Laboratory of Functional Aggregate Materials

References (43)

Show source-provided references
1. 10.1038/nrn205710.1038/nrn2057 ↗
2. 10.1038/nn.360210.1038/nn.3602 ↗
3. 10.1038/s41380-023-02018-x10.1038/s41380-023-02018-x ↗
4. 10.1038/nrdp.2017.210.1038/nrdp.2017.2 ↗
5. 10.1038/s41583-020-00382-z10.1038/s41583-020-00382-z ↗
6. 10.1038/nrn239110.1038/nrn2391 ↗
7. 10.1016/S0896-6273(00)00031-310.1016/S0896-6273(00)00031-3 ↗
8. 10.1038/s41592-024-02203-y10.1038/s41592-024-02203-y ↗
9. 10.1038/s41467-022-35355-610.1038/s41467-022-35355-6 ↗
10. 10.1083/jcb.103.1.17110.1083/jcb.103.1.171 ↗
11. 10.1038/s41467-019-13476-910.1038/s41467-019-13476-9 ↗
12. 10.1097/j.pain.000000000000322910.1097/j.pain.0000000000003229 ↗
13. 10.1007/s12035-018-0918-y10.1007/s12035-018-0918-y ↗
14. 10.1002/anie.20191672910.1002/anie.201916729 ↗
15. 10.1002/agt2.29710.1002/agt2.297 ↗
16. 10.1002/agt2.32710.1002/agt2.327 ↗
17. 10.1002/anie.20201639910.1002/anie.202016399 ↗
18. 10.1073/pnas.210614311810.1073/pnas.2106143118 ↗
19. 10.1002/agt2.31210.1002/agt2.312 ↗
20. 10.1126/sciadv.abn539010.1126/sciadv.abn5390 ↗
21. 10.1002/anie.20200589910.1002/anie.202005899 ↗
22. 10.1002/agt2.35210.1002/agt2.352 ↗
23. 10.1002/agt2.16010.1002/agt2.160 ↗
24. 10.1002/anie.20200074010.1002/anie.202000740 ↗
25. 10.1007/s11426-021-1199-610.1007/s11426-021-1199-6 ↗
26. 10.1039/C7SC04963C10.1039/C7SC04963C ↗
27. 10.1002/smll.20210177010.1002/smll.202101770 ↗
28. 10.1016/j.xcrp.2021.10073510.1016/j.xcrp.2021.100735 ↗
29. 10.1021/acs.analchem.0c0259610.1021/acs.analchem.0c02596 ↗
30. 10.1039/C9MH00906J10.1039/C9MH00906J ↗
31. 10.1016/j.crmeth.2023.10048610.1016/j.crmeth.2023.100486 ↗
32. 10.1016/0166-2236(89)90040-410.1016/0166-2236(89)90040-4 ↗
33. 10.3390/biom1405053610.3390/biom14050536 ↗
34. 10.1007/s00441-023-03769-310.1007/s00441-023-03769-3 ↗
35. 10.1038/s41582-023-00777-310.1038/s41582-023-00777-3 ↗
36. 10.1002/adma.20231060510.1002/adma.202310605 ↗
37. 10.1073/pnas.161164211310.1073/pnas.1611642113 ↗
38. 10.7150/thno.2308410.7150/thno.23084 ↗
39. 10.1002/adma.20230472410.1002/adma.202304724 ↗
40. 10.1126/sciadv.aax604010.1126/sciadv.aax6040 ↗
41. 10.1016/0304-3959(88)90209-610.1016/0304-3959(88)90209-6 ↗
42. 10.1038/s41577-019-0147-210.1038/s41577-019-0147-2 ↗
43. 10.1038/s41593-021-00975-610.1038/s41593-021-00975-6 ↗