Integrating Vascular Phenotypic and Proteomic Analysis in an Open Microfluidic Platform
Source abstract
Abstract
This research introduces a vascular phenotypic and proteomic analysis (VPT) platform designed to perform high-throughput experiments on vascular development. The VPT platform utilizes an open-channel configuration that facilitates angiogenesis by precise alignment of endothelial cells, allowing for a 3D morphological examination and protein analysis. We study the effects of antiangiogenic agents─bevacizumab, ramucirumab, cabozantinib, regorafenib, wortmannin, chloroquine, and paclitaxel─on cytoskeletal integrity and angiogenic sprouting, observing an approximately 50% reduction in sprouting at higher drug concentrations. Precise LC-MS/MS analyses reveal global protein expression changes in response to four of these drugs, providing insights into the signaling pathways related to the cell cycle, cytoskeleton, cellular senescence, and angiogenesis. Our findings emphasize the intricate relationship between cytoskeletal alterations and angiogenic responses, underlining the significance of integrating morphological and proteomic data for a comprehensive understanding of angiogenesis. The VPT platform not only advances our understanding of drug impacts on vascular biology but also offers a versatile tool for analyzing proteome and morphological features across various models beyond blood vessels.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Angiogenesis and VEGF in Cancer · Cancer Genomics and Diagnostics · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea
Target Link Therapeutics, Inc., Seoul 04545, Republic of Korea
Department of Public Health Science, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea
Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea
ProvaLabs, Inc., Seoul 08826, Republic of Korea
SOFT Foundry, Seoul National University, Seoul 08826, Republic of Korea
Inter-university Semiconductor Research Center, Seoul National University, Seoul 08826, Republic of Korea
Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do 13120, Republic of Korea
Institute of Advanced Machines and Design, Seoul National University, Seoul 08826, Republic of Korea
Qureator, Inc., San Diego, California 92121, United States
Funding
National Research Foundation of Korea · Gachon University
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