DataVXACS NanoPaper
journal article·2024-07-19

Portable Near-Infrared to Near-Infrared Platform for Homogeneous Quantification of Biomarkers in Complex Biological Samples

Yantao Li, Xiaorui Mai, Wenming Liu, Feng Wang, Shuo Yan, Yu Lei, Lu Chen, Weikang Mai, Qingwei Song, Weidong Du, Xukai Chen, Huiru Ye, Longfei Song, Yu Chen, Lei Zhao, Zhenwei Liu, Weidong Ding, Pei Yu, Xue Jiang, Yuyi Li, Jing Huang, Qiuqiang Zhan, Yiru Qin, Chenzhong Li, Wei Wei, Tianxing Ji

ACS Nano
Publisher page
Source abstract

Abstract

Förster resonance energy transfer (FRET)-based homogeneous immunoassay obviates tedious washing steps and thus is a promising approach for immunoassays. However, a conventional FRET-based homogeneous immunoassay operating in the visible region is not able to overcome the interference of complex biological samples, thus resulting in insufficient detection sensitivity and poor accuracy. Here, we develop a near-infrared (NIR)-to-NIR FRET platform (Ex = 808 nm, Em = 980 nm) that enables background-free high-throughput homogeneous quantification of various biomarkers in complex biological samples. This NIR-to-NIR FRET platform is portable and easy to operate and is mainly composed of a high-performance NIR-to-NIR FRET pair based on lanthanide-doped nanoparticles (LnNPs) and a custom-made microplate reader for readout of NIR luminescence signals. We demonstrate that this NIR-to-NIR FRET platform is versatile and robust, capable of realizing highly sensitive and accurate detection of various critical biomarkers, including small molecules (morphine and 1,25-dihydroxyvitamin D), proteins (human chorionic gonadotropin), and viral particles (adenovirus) in unprocessed complex biological samples (urine, whole blood, and feces) within 5-10 min. We expect this NIR-to-NIR FRET platform to provide low-cost healthcare for populations living in resource-limited areas and be widely used in many other fields, such as food safety and environmental monitoring.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Respiratory Disease, Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, P. R. China

MOE & Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. China

Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510535, P. R. China

Department of Pediatrics, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, P. R. China

Shenzhen Highcreation Technology Co., Ltd., Shenzhen 518122, P. R. China

State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, Hong Kong, 999077, P. R. China

Centre for Optical and Electromagnetic Research, Guangdong Engineering Research Centre of Optoelectronic Intelligent Information Perception, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510631, P. R. China

Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, P. R. China

Biomedical Engineering Division, Chinese University of Hong Kong (Shenzhen), Shenzhen 518172, China

Jvxin Tang Biotechnology Inc., Chengdu Future Medical City, Chengdu 610095, China

Funding

Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology · Basic and Applied Basic Research Foundation of Guangdong Province · National Natural Science Foundation of China · Clinical research project of Guangzhou Medical University Second Affiliated Hospitall · Plan on enhancing scientific research in GMU

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