Compressible Hollow Microlasers in Organoids for High-Throughput and Real-Time Mechanical Screening
Source abstract
Abstract
Mechanical stress within organoids is a pivotal indicator in disease modeling and pharmacokinetics, yet current tools lack the ability to rapidly and dynamically screen these mechanics. Here, we introduce biocompatible and compressible hollow microlasers that realize all-optical assessment of cellular stress within organoids. The laser spectroscopy yields identification of cellular deformation at the nanometer scale, corresponding to tens of pascals stress sensitivity. The compressibility enables the investigation of the isotropic component, which is the fundamental mechanics of multicellular models. By integrating with a microwell array, we demonstrate the high-throughput screening of mechanical cues in tumoroids, establishing a platform for mechano-responsive drug screening. Furthermore, we showcase the monitoring and mapping of dynamic contractile stress within human embryonic stem cell-derived cardiac organoids, revealing the internal mechanical inhomogeneity within a single organoid. This method eliminates time-consuming scanning and sample damage, providing insights into organoid mechanobiology.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Cellular Mechanics and Interactions · 3D Printing in Biomedical Research · Microfluidic and Bio-sensing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, Republic of Singapore
School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China
Department of Biological Sciences, National University of Singapore, 16 Science Drive 4, Singapore 117543, Singapore
Funding
Agency for Science, Technology and Research · Nanyang Technological University
References (57)
Show source-provided references
1. 10.1126/science.aaw756710.1126/science.aaw7567 ↗
2. 10.1126/science.aaw698510.1126/science.aaw6985 ↗
3. 10.1038/nrc254410.1038/nrc2544 ↗
4. 10.1038/nrm.2017.10810.1038/nrm.2017.108 ↗
5. 10.1038/s41586-020-2724-810.1038/s41586-020-2724-8 ↗
6. 10.1038/s41556-021-00699-610.1038/s41556-021-00699-6 ↗
7. 10.1088/1758-5090/ac2ef910.1088/1758-5090/ac2ef9 ↗
8. 10.1038/nmeth.153110.1038/nmeth.1531 ↗
9. 10.1002/adbi.20190012810.1002/adbi.201900128 ↗
10. 10.1021/acsbiomaterials.8b0123010.1021/acsbiomaterials.8b01230 ↗
11. 10.1038/s41551-016-000410.1038/s41551-016-0004 ↗
12. 10.1038/ncomms1405610.1038/ncomms14056 ↗
13. 10.1038/nmeth.276110.1038/nmeth.2761 ↗
14. 10.1038/s41467-018-04245-110.1038/s41467-018-04245-1 ↗
15. 10.1038/s41598-017-12363-x10.1038/s41598-017-12363-x ↗
16. 10.1039/C9SM02377A10.1039/C9SM02377A ↗
17. 10.1002/advs.20210241810.1002/advs.202102418 ↗
18. 10.1002/adhm.20200171610.1002/adhm.202001716 ↗
19. 10.1016/j.actbio.2021.12.03310.1016/j.actbio.2021.12.033 ↗
20. 10.1038/s43586-021-00079-210.1038/s43586-021-00079-2 ↗
21. 10.1002/adom.20190037710.1002/adom.201900377 ↗
22. 10.1038/s41551-017-0128-310.1038/s41551-017-0128-3 ↗
23. 10.1038/s41566-020-0631-z10.1038/s41566-020-0631-z ↗
24. 10.1021/acs.nanolett.2c0412310.1021/acs.nanolett.2c04123 ↗
25. 10.1038/s41467-022-28904-610.1038/s41467-022-28904-6 ↗
26. 10.1016/j.bios.2009.07.02110.1016/j.bios.2009.07.021 ↗
27. 10.1038/s41566-019-0489-010.1038/s41566-019-0489-0 ↗
28. 10.1038/nphoton.2015.12910.1038/nphoton.2015.129 ↗
29. 10.1073/pnas.231488412110.1073/pnas.2314884121 ↗
30. 10.1038/nbt0897-77810.1038/nbt0897-778 ↗
31. 10.1002/mabi.20160032810.1002/mabi.201600328 ↗
32. 10.1016/s0168-3659(98)00173-410.1016/s0168-3659(98)00173-4 ↗
33. 10.1163/15685620232081386410.1163/156856202320813864 ↗
34. 10.1016/j.actbio.2014.08.01510.1016/j.actbio.2014.08.015 ↗
35. 10.1016/0003-2697(87)90157-610.1016/0003-2697(87)90157-6 ↗
36. 10.1038/ncomms1552810.1038/ncomms15528 ↗
37. 10.1002/adom.20210142110.1002/adom.202101421 ↗
38. 10.1002/advs.20210355010.1002/advs.202103550 ↗
39. 10.1016/j.polymdegradstab.2011.07.01110.1016/j.polymdegradstab.2011.07.011 ↗
40. 10.1073/pnas.121335310910.1073/pnas.1213353109 ↗
41. 10.1038/s41467-018-07967-410.1038/s41467-018-07967-4 ↗
42. 10.1016/j.trecan.2018.02.00510.1016/j.trecan.2018.02.005 ↗
43. 10.1039/c3tb20441c10.1039/c3tb20441c ↗
44. 10.1038/nrm.2017.9810.1038/nrm.2017.98 ↗
45. 10.1038/nature1432910.1038/nature14329 ↗
46. 10.1146/annurev-bioeng-071813-10525910.1146/annurev-bioeng-071813-105259 ↗
47. 10.1115/1.403499110.1115/1.4034991 ↗
48. 10.1038/s41587-021-00815-910.1038/s41587-021-00815-9 ↗
49. 10.1038/s41596-021-00629-810.1038/s41596-021-00629-8 ↗
50. 10.1186/s13287-022-03215-110.1186/s13287-022-03215-1 ↗
51. 10.1038/s41586-018-0016-310.1038/s41586-018-0016-3 ↗
52. 10.1002/viw.2020008010.1002/viw.20200080 ↗
53. 10.1038/s41467-022-34860-y10.1038/s41467-022-34860-y ↗
54. 10.3390/app1119898110.3390/app11198981 ↗
55. 10.1039/C6SM02841A10.1039/C6SM02841A ↗
56. 10.1039/C9LC00872A10.1039/C9LC00872A ↗
57. 10.1038/nprot.2012.15010.1038/nprot.2012.150 ↗