journal article·2025-01-14

High-Definition, Video-Rate Triple-Channel NIR-II Imaging Using Shadowless Lamp Excitation and Illumination

Yuhuang Zhang, Shiyi Peng, Jian Guo, Jiayi Li, Zeyi Lu, Tianxiang Wu, Liying Chen, Wen Liu, Zhe Feng, Mingxi Zhang, Jun Qian

ACS Nano· Volume 19· Issue 1
Publisher page

Abstract

Multichannel imaging in the second near-infrared (NIR-II) window offers vital and comprehensive information for complex surgical environments, yet a simple, high-quality, video-rate multichannel imaging method with low safety risk remains to be proposed. Centered at the superior NIR-IIx window of 1400–1500 nm, triple-channel imaging coordinated with 1000–1100 and 1700–1880 nm (NIR-IIc) achieves exceptional clarity and an impressive signal-to-crosstalk ratio as high as 22.10. To further simplify the light source and lower the safety risk, we develop a type of in vivo multichannel imaging-assisted surgical navigation mode at a video frame rate of 25 fps under shadowless lamp excitation and illumination instead of extra excitation light sources. This work provides a reference for real-time, high-imaging-performance multichannel imaging with minimal crosstalk and introduces a practical fluorescence surgical navigation paradigm.
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Research context

OpenAlex topics: Nanoplatforms for cancer theranostics · Photoacoustic and Ultrasonic Imaging · Optical Imaging and Spectroscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Extreme Photonics and Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, China

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China

Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China

College of Physics and Electronic Information Engineering, Zhejiang Normal University, Jinhua 321004, China

Funding

National Key Research and Development Program of China · China Postdoctoral Science Foundation · Fundamental Research Funds for the Central Universities · National Natural Science Foundation of China · Natural Science Foundation of Zhejiang Province · Dr. Li Dak Sum & Yip Yio Chin Development Fund for Regenerative Medicine, Zhejiang University · University Laboratory Work Research Project of Zhejiang Province

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