Measuring the Maturity of Developing Cotton Fibers using an Automated Polarized Light Microscopy Technique

Cotton fibers are trichome cells composed primarily of cellulose. Mature fibers have more cellulose and a greater degree of cell wall thickening, and perform better than less mature fibers during textile processing. An automated polarized light microscope instrument called Siro-Mat that measures cotton fiber cell wall thickening was employed to assess the maturity of developing fibers from single cotton fruit. Fruit were taken from the first fruiting branch and position on glasshouse grown Gossypium hirsutum L. (Upland) and G. barbadense L. (Pima) plants, sequentially harvested from 24 days postanthesis (dpa) at approximately four-day intervals up until approximately 50 dpa. The instrument assessed an average of 13,000 fiber snippets per fruit. Upland fibers matured at a slower rate than Pima fibers up to 35 dpa. However, after 45 dpa Upland fibers had achieved a higher average maturity (i.e. 0.99 birefringence maturity index (BMI), cf. 0.79 for Pima). For both species the uniformity of fiber maturity increased as fibers matured up until 35 dpa for Upland and 29 dpa for Pima (i.e. the BMI coefficient of variation decreased as BMI increased during fruit development). It is envisaged that SiroMat will be a useful tool in helping to understand and manage fiber maturity by characterizing the maturation dynamics of cultivars with different inherent fiber properties, and for cultivars subjected to different environmental and agronomic conditions.