DataVXACS NanoPaper
journal article·2023-01-24

Defined Physicochemical Cues Steering Direct Neuronal Reprogramming on Colloidal Self-Assembled Patterns (cSAPs)

Javad Harati, Kun Liu, Hosein Shahsavarani, Ping Du, Massimiliano Galluzzi, Ke Deng, Jei Mei, Hsien-Yeh Chen, Shahin Bonakdar, Behrouz Aflatoonian, Guoqiang Hou, Yingjie Zhu, Haobo Pan, Raymond C. B. Wong, Mohammad Ali Shokrgozar, Weihong Song, Peng-Yuan Wang

ACS Nano· Volume 17· Issue 2
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Source abstract

Abstract

Direct neuronal reprogramming of somatic cells into induced neurons (iNs) has been recently established as a promising approach to generating neuron cells. Previous studies have reported that the biophysical cues of the in vitro microenvironment are potent modulators in the cell fate decision; thus, the present study explores the effects of a customized pattern (named colloidal self-assembled patterns, cSAPs) on iN generation from human fibroblasts using small molecules. The result revealed that the cSAP, composed of binary particles in a hexagonal-close-packed (hcp) geometry, is capable of improving neuronal reprogramming efficiency and steering the ratio of the iN subtypes. Cells exhibited distinct cell morphology, upregulated cell adhesion markers (i.e., SDC1 and ITGAV), enriched signaling pathways (i.e., Hippo and Wnt), and chromatin remodeling on the cSAP compared to those on the control substrates. The result also showed that the iN subtype specification on cSAP was surface-dependent; therefore, the defined physicochemical cue from each cSAP is exclusive. Our findings show that direct cell reprogramming can be manipulated through specific biophysical cues on the artificial matrix, which is significant in cell transdifferentiation and lineage conversion.
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Research context

OpenAlex topics: Pluripotent Stem Cells Research · 3D Printing in Biomedical Research · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Lab Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, Tehran1316943551, Iran

Oujiang Laboratory, Key Laboratory of Alzheimer’s Disease of Zhejiang Province, Institute of Aging, Wenzhou Medical University, Wenzhou, Zhejiang325000, People’s Republic of China

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong518055, People’s Republic of China

University of Chinese Academy of Science, Beijing101408, People’s Republic of China

Department of Cell and Molecular Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran1983969411, Iran

Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong518055, People’s Republic of China

School of Food and Bioengineering, Xihua University, Chengdu610097, People’s Republic of China

Department of Chemical Engineering, National Taiwan University, Taipei10617, Taiwan

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd8916188635, Iran

Shenzhen Key Laboratory of Drug Addiction, Shenzhen Neher Neural Plasticity Laboratory, the Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, Guangdong518055, People’s Republic of China

Centre for Eye Research Australia, Department of Surgery, University of Melbourne, Parkville, Victoria3002, Australia

Funding

Guangdong Science and Technology Department · Ministry of Science and Technology of the People's Republic of China · Science, Technology and Innovation Commission of Shenzhen Municipality · National Natural Science Foundation of China · Chinese Academy of Sciences · Natural Science Foundation of Zhejiang Province

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