Identifying Viral Vector Characteristics by Nanopore Sensing

Makusu Tsutsui; Mikako Wada; Akihide Arima; Yuji Tsunekawa; Takako Sasaki; Kenji Sakamoto; Kazumichi Yokota; Yoshinobu Baba; Tomoji Kawai; Takashi Okada

doi:10.1021/acsnano.4c01888

journal article·2024-06-18

Identifying Viral Vector Characteristics by Nanopore Sensing

Makusu Tsutsui, Mikako Wada, Akihide Arima, Yuji Tsunekawa, Takako Sasaki, Kenji Sakamoto, Kazumichi Yokota, Yoshinobu Baba, Tomoji Kawai, Takashi Okada

ACS Nano· Volume 18· Issue 24

Publisher page

Source abstract

Abstract

Using viral vectors as gene delivery vehicles for gene therapy necessitates their quality control. Here, we report on nanopore sensing for nondestructively inspecting genomes inside the nanoscale cargoes at the single-molecule level. Using ionic current measurements, we motion-tracked the adeno-associated virus (AAV) vectors as they translocated through a solid-state nanopore. Considering the varying contributions of the electrophoretic forces from the negatively charged internal polynucleotides of different lengths, the nanocargoes carrying longer DNA moved more slowly in the nanochannel. Moreover, ion blockage characteristics revealed their larger volume by up to approximately 3600 nm 3 in proportion to the length of single-stranded DNA packaged inside, thereby allowing electrical discriminations of AAV vectors by the gene-derived physical features. The present findings can be a promising tool for the enhanced quality control of AAV products by enabling the screening of empty and intermediate vectors at the single-particle level.

Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Nanopore and Nanochannel Transport Studies · RNA Interference and Gene Delivery · Energy Harvesting in Wireless Networks

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan

Division of Molecular and Medical Genetics, Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan

Institute of Nano-Life-Systems Institutes of Innovation for Future Society, Nagoya University, Nagoya 464-8603, Japan

National Institute of Advanced Industrial Science and Technology, Kagawa 761-0395, Japan

Department of Biomolecular Engineering Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan

Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan

Funding

Japan Agency for Medical Research and Development · Japan Society for the Promotion of Science

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