DataVXACS NanoPaper
journal article·2024-04-09

Enhancing Specific Fluorescence In Situ Hybridization with Quantum Dots for Single-Molecule RNA Imaging in Formalin-Fixed Paraffin-Embedded Tumor Tissues

Zeyin Zhao, Mengyuan Jiang, Chen He, Wenjuan Yin, Yawei Feng, Peng Wang, Lisha Ying, Ting Fu, Dan Su, Ruizi Peng, Weihong Tan

ACS Nano· Volume 18· Issue 14
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Source abstract

Abstract

Single-molecule fluorescence in situ hybridization (smFISH) represents a promising approach for the quantitative analysis of nucleic acid biomarkers in clinical tissue samples. However, low signal intensity and high background noise are complications that arise from diagnostic pathology when performed with smFISH-based RNA imaging in formalin-fixed paraffin-embedded (FFPE) tissue specimens. Moreover, the associated complex procedures can produce uncertain results and poor image quality. Herein, by combining the high specificity of split DNA probes with the high signal readout of ZnCdSe/ZnS quantum dot (QD) labeling, we introduce QD split-FISH, a high-brightness smFISH technology, to quantify the expression of mRNA in both cell lines and clinical FFPE tissue samples of breast cancer and lung squamous carcinoma. Owing to its high signal-to-noise ratio, QD split-FISH is a fast, inexpensive, and sensitive method for quantifying mRNA expression in FFPE tumor tissues, making it suitable for biomarker imaging and diagnostic pathology.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Single-cell and spatial transcriptomics · Advanced biosensing and bioanalysis techniques · Anodic Oxide Films and Nanostructures

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China

Department of Pathology, Zhejiang Cancer Hospital, Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China

School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui 230026, China

School of Molecular Medicine, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

Funding

Natural Science Foundation of Hunan Province · National Natural Science Foundation of China · Natural Science Foundation of Zhejiang Province · Zhejiang Leading Innovation and Entrepreneurship Team · Zhejiang Kunpeng Program

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