DataVXACS NanoPaper
journal article·2022-09-27

Color-Switchable Nanosilicon Fluorescent Probes

Huai Chen, Jiang Xu, Yaping Wang, Da Wang, Raquel Ferrer-Espada, Yutong Wang, Jingjian Zhou, Adrián Pedrazo-Tardajos, Mei Yang, Jia-Heng Tan, Xiaoyu Yang, Lei Zhang, Ilya Sychugov, Shoudeng Chen, Sara Bals, Johan Paulsson, Zhenyu Yang

ACS Nano· Volume 16· Issue 9
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Source abstract

Abstract

Fluorescent probes are vital to cell imaging by allowing specific parts of cells to be visualized and quantified. Color-switchable probes (CSPs), with tunable emission wavelength upon contact with specific targets, are particularly powerful because they not only eliminate the need to wash away all unbound probe but also allow for internal controls of probe concentrations, thereby facilitating quantification. Several such CSPs exist and have proven very useful, but not for all key cellular targets. Here we report a pioneering CSP for in situ cell imaging using aldehyde-functionalized silicon nanocrystals (SiNCs) that switch their intrinsic photoluminescence from red to blue quickly when interacting with amino acids in live cells. Though conventional probes often work better in cell-free extracts than in live cells, the SiNCs display the opposite behavior and function well and fast in universal cell lines at 37 °C while requiring much higher temperature in extracts. Furthermore, the SiNCs only disperse in cytoplasm not nucleus, and their fluorescence intensity correlated linearly with the concentration of fed amino acids. We believe these nanosilicon probes will be promising tools to visualize distribution of amino acids and potentially quantify amino acid related processes in live cells.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Silicon Nanostructures and Photoluminescence · Carbon and Quantum Dots Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

MOE Laboratory of Bioinorganic and Synthetic Chemistry, Lehn Institute of Functional Materials, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China

Department of Systems Biology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States

EMAT and NANOlab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium

Guangdong Provincial Key Laboratory of Biomedical Imaging, Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China

Department of Applied Physics, KTH Royal Institute of Technology, 11419 Stockholm, Sweden

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510275, China

Department of Molecular Medicine, School of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, United States

Department of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L3G1, Canada

Funding

Guangdong Science and Technology Department · Sun Yat-sen University · National Natural Science Foundation of China · Natural Science Foundation of Guangdong Province · EU Infrastructure Project EUSMI · ERC Consolidator Grant · Marie Sklodowska-Curie Actions (MSCA) in Horizon 2020 Programme

References (41)

Show source-provided references
1. 10.1021/ar100063310.1021/ar1000633 ↗
2. 10.1016/j.cbpa.2016.04.00410.1016/j.cbpa.2016.04.004 ↗
3. 10.1021/ja310317n10.1021/ja310317n ↗
4. 10.1021/acs.jpcc.7b0083310.1021/acs.jpcc.7b00833 ↗
5. 10.1021/ja110546410.1021/ja1105464 ↗
6. 10.1038/nbt89610.1038/nbt896 ↗
7. 10.1021/jacs.9b1108010.1021/jacs.9b11080 ↗
8. 10.1039/C6SC04842K10.1039/C6SC04842K ↗
9. 10.1126/science.281.5385.201310.1126/science.281.5385.2013 ↗
10. 10.1126/science.281.5385.201610.1126/science.281.5385.2016 ↗
11. 10.1038/nmat139010.1038/nmat1390 ↗
12. 10.1126/science.108852510.1126/science.1088525 ↗
13. 10.1002/smll.20110084510.1002/smll.201100845 ↗
14. 10.1039/C6TC01159D10.1039/C6TC01159D ↗
15. 10.1021/nn700319z10.1021/nn700319z ↗
16. 10.1002/anie.20150606510.1002/anie.201506065 ↗
17. 10.1038/nmat239810.1038/nmat2398 ↗
18. 10.1039/C3CS60353A10.1039/C3CS60353A ↗
19. 10.1021/ja402622710.1021/ja4026227 ↗
20. 10.1038/lsa.2013.310.1038/lsa.2013.3 ↗
21. 10.1021/nn504109a10.1021/nn504109a ↗
22. 10.1021/nn400064410.1021/nn4000644 ↗
23. 10.1016/j.jare.2010.05.00410.1016/j.jare.2010.05.004 ↗
24. 10.1039/C5EE02506K10.1039/C5EE02506K ↗
25. 10.2174/13852720978719377410.2174/138527209787193774 ↗
26. 10.1016/j.carbpol.2019.11525610.1016/j.carbpol.2019.115256 ↗
27. 10.1021/cr000064s10.1021/cr000064s ↗
28. 10.1038/srep2104210.1038/srep21042 ↗
29. 10.1103/PhysRevB.93.16141310.1103/PhysRevB.93.161413 ↗
30. 10.1016/S0257-8972(97)00229-610.1016/S0257-8972(97)00229-6 ↗
31. 10.1016/j.apcata.2010.09.00910.1016/j.apcata.2010.09.009 ↗
32. 10.1016/0021-9797(87)90323-710.1016/0021-9797(87)90323-7 ↗
33. 10.1002/pssa.20120027310.1002/pssa.201200273 ↗
34. 10.1021/acsphotonics.5b0014310.1021/acsphotonics.5b00143 ↗
35. 10.1083/jcb.74.2.46810.1083/jcb.74.2.468 ↗
36. 10.3389/fcell.2017.0010610.3389/fcell.2017.00106 ↗
37. 10.1073/pnas.54.3.88010.1073/pnas.54.3.880 ↗
38. 10.1021/jacs.6b0081610.1021/jacs.6b00816 ↗
39. 10.1038/nmeth.166310.1038/nmeth.1663 ↗
40. 10.1021/acs.accounts.6b0029210.1021/acs.accounts.6b00292 ↗
41. 10.1021/acs.chemmater.6b0266710.1021/acs.chemmater.6b02667 ↗