Antibiotic-Loaded Polymersomes for Clearance of Intracellular Burkholderia thailandensis
Source abstract
Abstract
Melioidosis caused by the facultative intracellular pathogen Burkholderia pseudomallei is difficult to treat due to poor intracellular bioavailability of antibiotics and antibiotic resistance. In the absence of novel compounds, polymersome (PM) encapsulation may increase the efficacy of existing antibiotics and reduce antibiotic resistance by promoting targeted, infection-specific intracellular uptake. In this study, we developed PMs composed of widely available poly(ethylene oxide)-polycaprolactone block copolymers and demonstrated their delivery to intracellular B. thailandensis infection using multispectral imaging flow cytometry (IFC) and coherent anti-Stokes Raman scattering microscopy. Antibiotics were tightly sequestered in PMs and did not inhibit the growth of free-living B. thailandensis. However, on uptake of antibiotic-loaded PMs by infected macrophages, IFC demonstrated PM colocalization with intracellular B. thailandensis and a significant inhibition of their growth. We conclude that PMs are a viable approach for the targeted antibiotic treatment of persistent intracellular Burkholderia infection.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Burkholderia infections and melioidosis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Bioengineering Sciences Group, Faculty of Engineering and the Environment, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom
Centre for Human Development, Stem Cells and Regeneration, Bone and Joint Research Group, University of Southampton Faculty of Medicine, Southampton SO16 6YD,United Kingdom
Clinical and Experimental Sciences, Faculty of Medicine, Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom
Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom
Chemical, Biological, and Radiological Division, Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, United Kingdom
London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom
Funding
Engineering and Physical Sciences Research Council · University of Southampton · Ministry of Defence
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