journal article·2022-04-26

Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity

Fengyan Song, Xinwen Ou, Tsu Yu Chou, Junkai Liu, Hui Gao, Ruoyao Zhang, Xiaolin Huang, Zujin Zhao, Jianwei Sun, Sijie Chen, Jacky W. Y. Lam, Ben Zhong Tang

ACS Nano· Volume 16· Issue 4
Publisher page

Abstract

Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.
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Research context

OpenAlex topics: Nanoplatforms for cancer theranostics · Luminescence and Fluorescent Materials · Advanced Fluorescence Microscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China

Department of Chemical Biology, School of Life Science and Chemistry, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China

Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong 999077, China

School of Aeronautic Science and Engineering, Beihang University, Beijing 100191, China

Institute of Engineering Medicine, Beijing Institute of Technology, Beijing 100081, China

State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China

State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, China

Funding

Beihang University · Research Grants Council, University Grants Committee · Innovation and Technology Commission · National Natural Science Foundation of China · Karolinska Institutet · Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet

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