Incorporation of Glucose Oxidase into Conducting Organic Langmuir-Blodgett Films

Conducting organic Langmuir-Blodgett films (LB) were prepared and their ability to immobilize a protein molecule was discussed. The conducting LB films are obtained by mixing bis-ethylenedioxy-tetrathiafulvalene (BO) molecule with fatty acid (FA). As an enzyme, glucose oxidase (GOD) was immobilized on the conducting LB film. Molecular orientations and other characterization of GOD-LB complexes were investigated by infrared absorption technique. To discuss the use of the conducting LB films for fabricating a glucose sensor, we investigate the change of the FT-IR in GOD-LB complexes after injection of glucose. GOD is absorbed in the LB films and maintains its bioactivity and sensitivity. The present technique with highly ordered conducting LB films is to be used as a suitable platform for immobilization of the enzyme for sensor architecture.