journal article·2016-04-26

Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions

In Soo Park, Tae Joon Kwak, Gyudo Lee, Myeonggu Son, Jeong Woo Choi, Seungyeop Choi, Kihwan Nam, Sei-Young Lee, Woo-Jin Chang, Kilho Eom, Dae Sung Yoon, Sangyoup Lee, Rashid Bashir, Sang Woo Lee

ACS Nano· Volume 10· Issue 4
Publisher page

Abstract

The direct quantification of weak intermolecular binding interactions is very important for many applications in biology and medicine. Techniques that can be used to investigate such interactions under a controlled environment, while varying different parameters such as loading rate, pulling direction, rupture event measurements, and the use of different functionalized probes, are still lacking. Herein, we demonstrate a biaxial dielectrophoresis force spectroscopy (BDFS) method that can be used to investigate weak unbinding events in a high-throughput manner under controlled environments and by varying the pulling direction (i.e., transverse and/or vertical axes) as well as the loading rate. With the BDFS system, we can quantitatively analyze binding interactions related to hydrogen bonding or ionic attractions between functionalized microbeads and a surface within a microfluidic device. Our BDFS system allowed for the characterization of the number of bonds involved in an interaction, bond affinity, kinetic rates, and energy barrier heights and widths from different regimes of the energy landscape.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Force Microscopy Techniques and Applications · Microfluidic and Bio-sensing Technologies · Mechanical and Optical Resonators

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering, Yonsei University, Wonju 220-710, Korea

Mechanical Engineering Department, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin 53211, United States

T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts 02115, United States

Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 136-791, Korea

Biomechanics Laboratory, College of Sport Science, Sungkyunkwan University, Suwon 440-746, Korea

Department of Bio-convergence Engineering, Korea University, Seoul 136-703, Korea

Department of Biomedical Engineering, University of Science and Technology, Daejeon 305-350, Korea

Department of Bioengineering, Micro and Nanotechnology Laboratory, University of Illinois at Urbana—Champaign Champaign, Illinois 61801, United States

Funding

National Research Foundation of Korea · Yonsei University

References (35)

Show source-provided references
1. 10.1146/annurev.biochem.72.121801.16154210.1146/annurev.biochem.72.121801.161542 ↗
2. 10.1126/science.123104110.1126/science.1231041 ↗
3. 10.1016/S0959-440X(97)80082-610.1016/S0959-440X(97)80082-6 ↗
4. 10.1073/pnas.95.13.740210.1073/pnas.95.13.7402 ↗
5. 10.1073/pnas.23045169710.1073/pnas.230451697 ↗
6. 10.1073/pnas.060334210310.1073/pnas.0603342103 ↗
7. 10.1016/S0006-3495(02)75672-510.1016/S0006-3495(02)75672-5 ↗
8. 10.1038/nnano.2010.21210.1038/nnano.2010.212 ↗
9. 10.1126/science.34757510.1126/science.347575 ↗
10. 10.1146/annurev.cellbio.042308.11323810.1146/annurev.cellbio.042308.113238 ↗
11. 10.1126/science.276.5315.110910.1126/science.276.5315.1109 ↗
12. 10.1103/PhysRevE.71.02190410.1103/PhysRevE.71.021904 ↗
13. 10.1146/annurev.biochem.77.060706.09310210.1146/annurev.biochem.77.060706.093102 ↗
14. 10.1016/S0079-6107(00)00014-610.1016/S0079-6107(00)00014-6 ↗
15. 10.1146/annurev.biophys.36.101106.10145110.1146/annurev.biophys.36.101106.101451 ↗
16. 10.1016/S0003-2670(02)01569-610.1016/S0003-2670(02)01569-6 ↗
17. 10.1073/pnas.060299510310.1073/pnas.0602995103 ↗
18. 10.1038/nsb96510.1038/nsb965 ↗
19. 10.1021/jp075955j10.1021/jp075955j ↗
20. 10.1073/pnas.070545810410.1073/pnas.0705458104 ↗
21. 10.1002/jmr.84110.1002/jmr.841 ↗
22. 10.1103/PhysRevLett.104.10830110.1103/PhysRevLett.104.108301 ↗
23. 10.1063/1.274448310.1063/1.2744483 ↗
24. 10.1021/ac901211b10.1021/ac901211b ↗
25. 10.1021/nn302202t10.1021/nn302202t ↗
26. 10.1016/S0006-3495(93)81277-310.1016/S0006-3495(93)81277-3 ↗
27. 10.1016/S0006-3495(90)82357-210.1016/S0006-3495(90)82357-2 ↗
28. 10.1103/PhysRevE.78.02190510.1103/PhysRevE.78.021905 ↗
29. 10.1038/ncomms786110.1038/ncomms7861 ↗
30. 10.1038/1621910.1038/16219 ↗
31. 10.1016/j.jcis.2007.02.08410.1016/j.jcis.2007.02.084 ↗
32. 10.1063/1.273592010.1063/1.2735920 ↗
33. 10.1109/MEMB.2003.130499910.1109/MEMB.2003.1304999 ↗
34. 10.1021/la950500j10.1021/la950500j ↗
35. 10.1021/ac961065g10.1021/ac961065g ↗