DataVXACS NanoPaper
journal article·2023-10-24

Enhanced Neuron Growth and Electrical Activity by a Supramolecular Netrin-1 Mimetic Nanofiber

Cara S. Smith, Zaida Álvarez, Ruomeng Qiu, Ivan R. Sasselli, Tristan Clemons, J. Alberto Ortega, Marcos Vilela-Picos, Haley Wellman, Evangelos Kiskinis, Samuel I. Stupp

ACS Nano· Volume 17· Issue 20
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Source abstract

Abstract

Neurotrophic factors are essential not only for guiding the organization of the developing nervous system but also for supporting the survival and growth of neurons after traumatic injury. In the central nervous system (CNS), inhibitory factors and the formation of a glial scar after injury hinder the functional recovery of neurons, requiring exogenous therapies to promote regeneration. Netrin-1, a neurotrophic factor, can initiate axon guidance, outgrowth, and branching, as well as synaptogenesis, through activation of deleted in colorectal cancer (DCC) receptors. We report here the development of a nanofiber-shaped supramolecular mimetic of netrin-1 with monomers that incorporate a cyclic peptide sequence as the bioactive component. The mimetic structure was found to activate the DCC receptor in primary cortical neurons using low molar ratios of the bioactive comonomer. The supramolecular nanofibers enhanced neurite outgrowth and upregulated maturation as well as pre- and postsynaptic markers over time, resulting in differences in electrical activity similar to neurons treated with the recombinant netrin-1 protein. The results suggest the possibility of using the supramolecular structure as a therapeutic to promote regenerative bioactivity in CNS injuries.
Source: openalex_inverted_index · Quality: baseline

Research context

NanomaterialsBiomaterials

OpenAlex topics: Axon Guidance and Neuronal Signaling · Nerve injury and regeneration · Neurogenesis and neuroplasticity mechanisms

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Simpson Querrey Institute for BioNanotechnology, Northwestern University, Chicago, Illinois 60611, United States

Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States

Department of Medicine, Northwestern University, Chicago, Illinois 60611, United States

Biomaterials for Neural Regeneration, Institute for Bioengineering of Catalonia (IBEC), Barcelona 08028, Spain

Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States

Centro de Fisica de Materiales (CFM), CSIC-UPV/EHU, San Sebastián 20018, Spain

The Ken & Ruth Davee Department of Neurology, Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States

Department of Pathology and Experimental Therapeutics, Institute of Neurosciences, University of Barcelona, L’Hospitalet de Llobregat, Barcelona 08907, Spain

Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States

Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States

Funding

National Institute of Neurological Disorders and Stroke · Ministerio de Universidades · Ministerio de Ciencia e Innovaci?n · Division of Graduate Education · National Institute of Biomedical Imaging and Bioengineering · New York Stem Cell Foundation · National Institute on Aging · American Australian Association · Center for Regenerative Nanomedicine

References (89)

Show source-provided references
1. 10.1038/367170a010.1038/367170a0 ↗
2. 10.1038/350230a010.1038/350230a0 ↗
3. 10.1016/S0896-6273(00)80266-410.1016/S0896-6273(00)80266-4 ↗
4. 10.1016/0092-8674(91)90396-G10.1016/0092-8674(91)90396-G ↗
5. 10.1038/3503955910.1038/35039559 ↗
6. 10.1126/science.849355710.1126/science.8493557 ↗
7. 10.1073/pnas.180473511510.1073/pnas.1804735115 ↗
8. 10.1038/s41586-018-0467-610.1038/s41586-018-0467-6 ↗
9. 10.1038/sj.cdd.440166210.1038/sj.cdd.4401662 ↗
10. 10.1038/373344a010.1038/373344a0 ↗
11. 10.1038/373339a010.1038/373339a0 ↗
12. 10.1016/j.jconrel.2006.05.00510.1016/j.jconrel.2006.05.005 ↗
13. 10.1021/acsbiomaterials.0c0002310.1021/acsbiomaterials.0c00023 ↗
14. 10.1523/JNEUROSCI.2769-09.200910.1523/JNEUROSCI.2769-09.2009 ↗
15. 10.1523/JNEUROSCI.17-14-05560.199710.1523/JNEUROSCI.17-14-05560.1997 ↗
16. 10.2174/138920101766616082314403210.2174/1389201017666160823144032 ↗
17. 10.1016/j.jconrel.2019.02.01610.1016/j.jconrel.2019.02.016 ↗
18. 10.1038/nmat447410.1038/nmat4474 ↗
19. 10.1126/science.106318710.1126/science.1063187 ↗
20. 10.1021/la903096910.1021/la9030969 ↗
21. 10.1126/science.aat614110.1126/science.aat6141 ↗
22. 10.1038/ncomms1598210.1038/ncomms15982 ↗
23. 10.1038/ncomms1156110.1038/ncomms11561 ↗
24. 10.1039/C7CS00735C10.1039/C7CS00735C ↗
25. 10.1021/acsami.3c0244710.1021/acsami.3c02447 ↗
26. 10.1126/science.abh360210.1126/science.abh3602 ↗
27. 10.1016/j.biomaterials.2013.09.07710.1016/j.biomaterials.2013.09.077 ↗
28. 10.1523/JNEUROSCI.0143-08.200810.1523/JNEUROSCI.0143-08.2008 ↗
29. 10.1016/j.actbio.2016.04.01010.1016/j.actbio.2016.04.010 ↗
30. 10.1002/term.264410.1002/term.2644 ↗
31. 10.1073/pnas.101654610810.1073/pnas.1016546108 ↗
32. 10.1093/brain/awy32810.1093/brain/awy328 ↗
33. 10.1021/acsbiomaterials.7b0034710.1021/acsbiomaterials.7b00347 ↗
34. 10.1021/acs.nanolett.8b0231710.1021/acs.nanolett.8b02317 ↗
35. 10.1016/j.stem.2022.12.01010.1016/j.stem.2022.12.010 ↗
36. 10.1016/0092-8674(94)90420-010.1016/0092-8674(94)90420-0 ↗
37. 10.1016/S0092-8674(00)81795-X10.1016/S0092-8674(00)81795-X ↗
38. 10.1016/S0896-6273(00)80413-410.1016/S0896-6273(00)80413-4 ↗
39. 10.1016/j.mcn.2004.11.00910.1016/j.mcn.2004.11.009 ↗
40. 10.1242/dev.124.24.506310.1242/dev.124.24.5063 ↗
41. 10.1016/S0896-6273(00)81116-210.1016/S0896-6273(00)81116-2 ↗
42. 10.1523/JNEUROSCI.4963-03.200410.1523/JNEUROSCI.4963-03.2004 ↗
43. 10.1523/JNEUROSCI.1085-13.201310.1523/JNEUROSCI.1085-13.2013 ↗
44. 10.1523/JNEUROSCI.0947-09.200910.1523/JNEUROSCI.0947-09.2009 ↗
45. 10.1038/s41467-017-00652-y10.1038/s41467-017-00652-y ↗
46. 10.1016/j.neuron.2014.07.01010.1016/j.neuron.2014.07.010 ↗
47. 10.1038/nature74810.1038/nature748 ↗
48. 10.1016/S0092-8674(00)81336-710.1016/S0092-8674(00)81336-7 ↗
49. 10.1083/jcb.20040505310.1083/jcb.200405053 ↗
50. 10.1007/s11684-011-0118-x10.1007/s11684-011-0118-x ↗
51. 10.1016/j.nbd.2011.06.00610.1016/j.nbd.2011.06.006 ↗
52. 10.1038/cdd.2008.19110.1038/cdd.2008.191 ↗
53. 10.3233/JAD-15104610.3233/JAD-151046 ↗
54. 10.1152/ajpcell.00332.201410.1152/ajpcell.00332.2014 ↗
55. 10.1039/C4BM00326H10.1039/C4BM00326H ↗
56. 10.1038/nmat453810.1038/nmat4538 ↗
57. 10.1007/978-1-4615-1863-1_810.1007/978-1-4615-1863-1_8 ↗
58. 10.1021/acsomega.0c0027110.1021/acsomega.0c00271 ↗
59. 10.1021/acsami.8b1365310.1021/acsami.8b13653 ↗
60. 10.1016/S0896-6273(00)80760-610.1016/S0896-6273(00)80760-6 ↗
61. 10.1006/mcne.2001.107510.1006/mcne.2001.1075 ↗
62. 10.1074/jbc.M10991320010.1074/jbc.M109913200 ↗
63. 10.1016/j.molmed.2012.07.00110.1016/j.molmed.2012.07.001 ↗
64. 10.1016/0092-8674(94)90421-910.1016/0092-8674(94)90421-9 ↗
65. 10.1523/JNEUROSCI.5191-05.200610.1523/JNEUROSCI.5191-05.2006 ↗
66. 10.1083/jcb.20131100310.1083/jcb.201311003 ↗
67. 10.1016/j.celrep.2012.12.00510.1016/j.celrep.2012.12.005 ↗
68. 10.1186/gb-2004-6-1-20410.1186/gb-2004-6-1-204 ↗
69. 10.1016/j.neuroscience.2008.08.01510.1016/j.neuroscience.2008.08.015 ↗
70. 10.1186/1758-2946-4-1710.1186/1758-2946-4-17 ↗
71. 10.1021/jp036508g10.1021/jp036508g ↗
72. 10.1021/jp071097f10.1021/jp071097f ↗
73. 10.1021/ct700324x10.1021/ct700324x ↗
74. 10.1021/ct300646g10.1021/ct300646g ↗
75. 10.1002/jnr.2247210.1002/jnr.22472 ↗
76. 10.1021/nl302487m10.1021/nl302487m ↗
77. 10.1002/smll.20200574310.1002/smll.202005743 ↗
78. 10.1021/acs.jpcb.1c0925810.1021/acs.jpcb.1c09258 ↗
79. 10.1038/nchem.212210.1038/nchem.2122 ↗
80. 10.1021/nn300015g10.1021/nn300015g ↗
81. 10.1002/chem.20150179310.1002/chem.201501793 ↗
82. 10.1016/j.softx.2015.06.00110.1016/j.softx.2015.06.001 ↗
83. 10.1016/0263-7855(96)00018-510.1016/0263-7855(96)00018-5 ↗
84. 10.1016/j.cpc.2015.09.01410.1016/j.cpc.2015.09.014 ↗
85. 10.1063/1.240842010.1063/1.2408420 ↗
86. 10.1063/1.44811810.1063/1.448118 ↗
87. 10.1093/cercor/bhp27510.1093/cercor/bhp275 ↗
88. XonaChips® 2-Compartment XC Series. Xona Microfluidics. https://xonamicrofluidics.com/protocols/ (accessed 2020-09-14).
89. QBM Cell Science – E14/15 Embryonic C57 Mouse Cortical Neurons. Axion BioSystems. https://www.axionbiosystems.com/resources/culture-protocol/qbm-cell-science-e1415-embryonic-c57-mouse-cortical-neurons (accessed 2023-02-07).