Entry Dynamics of Single Ebola Virus Revealed by Force Tracing

Qingrong Zhang; Falin Tian; Fei Wang; Zhengyuan Guo; Mingjun Cai; Haijiao Xu; Hongda Wang; Guocheng Yang; Xinghua Shi; Yuping Shan; Zongqiang Cui

doi:10.1021/acsnano.0c01739

journal article·2020-06-23Open access

Entry Dynamics of Single Ebola Virus Revealed by Force Tracing

Qingrong Zhang, Falin Tian, Fei Wang, Zhengyuan Guo, Mingjun Cai, Haijiao Xu, Hongda Wang, Guocheng Yang, Xinghua Shi, Yuping Shan, Zongqiang Cui

ACS Nano· Volume 14· Issue 6

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Source abstract

Abstract

Infections by the Ebola virus (EBOV) rapidly cause fatal hemorrhagic fever in humans. Viral entry into host cells is the most critical step in infection and an attractive target for therapeutic intervention. Herein, the invagination behavior and entry dynamics of filamentous Ebola virus-like particles (EBO-VLPs) were investigated using a force tracing technique based on atomic force microscopy and single-particle fluorescence tracking in real time. The filamentous EBOV-VLPs might enter cells in both horizontal and vertical modes, and the virus-receptor interactions during endocytic uptake were analyzed. In addition, molecular dynamics simulations and engulfment energy analysis further depicted EBO-VLP entry in the horizontal and vertical directions and suggested that internalization in the vertical direction requires a larger force and more time. This report provides useful information for further revealing the mechanism of viral infection, which is important for understanding viral pathogenesis.

Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Viral Infections and Outbreaks Research · COVID-19 epidemiological studies · SARS-CoV-2 and COVID-19 Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, P.R. China

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China

Laboratory of Theoretical and Computational Nanoscience, CAS Key Laboratory for Nanosystem and Hierarchy Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing 100190, P.R. China

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, P.R. China

University of Chinese Academy of Sciences, Beijing 100049, P.R. China

Funding

Ministry of Science and Technology of the People's Republic of China · K. C. Wong Education Foundation · China Postdoctoral Science Foundation · National Natural Science Foundation of China · Chinese Academy of Sciences · State Key Laboratory of Nonlinear Mechanics, Chinese Academy of Sciences

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