journal article·2010-03-23

Controlled Assembly for Well-Defined 3D Bioarchitecture Using Two Active Enzymes

Dong Chung Kim, Jung Inn Sohn, Dejian Zhou, Thomas A. J. Duke, Dae Joon Kang

ACS Nano· Volume 4· Issue 3
Publisher page

Abstract

This paper reports that a bioarchitecture with two different active enzymes can be fabricated conveniently on a prepatterned surface by highly selective surface-templated layer-by-layer (LBL) assembly by coupling a bilayer of avidin/biotin-lactate oxidase (biotin-LOD) with a bilayer of avidin/biotin-horseradish peroxidase (biotin-HRP). The two different active enzymes can be utilized as excellent building blocks for the fabrication of well-defined 3D nanostructures with precise control of the position and height on the surface. In addition, the LBL assembled bienzyme structures are highly functional, and bioarchitectures based on LOD and HRP-mediated coupling reaction can be employed in a number of viable biosensing applications.
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Research context

OpenAlex topics: Diatoms and Algae Research · Nanofabrication and Lithography Techniques · Molecular Junctions and Nanostructures

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

BK21 Physics Research Division, Department of Energy Science, Institute of Basic Science, SKKU Advanced Institute of Nanotechnology, Center for Nanotubes and Nanostructured Composites, Sungkyunkwan University, Suwon, 440-746, South Korea

Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, 19 J J Thomson Avenue, Cambridge CB3 0HE, U.K.

Department of Human Nutrition and Food Science, Chungwoon University, Hongseong 350-701, South Korea

Nanoscience Centre, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U.K.

School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, U.K.

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