DataVXACS NanoPaper
journal article·2021-07-27Open access

Stealth Effect of Short Polyoxazolines in Graft Copolymers: Minor Changes of Backbone End Group Determine Liver Cell-Type Specificity

Irina Muljajew, Sophie Huschke, Anuradha Ramoji, Zoltán Cseresnyés, Stephanie Hoeppener, Ivo Nischang, Wanling Foo, Jürgen Popp, Marc Thilo Figge, Christine Weber, Michael Bauer, Ulrich S. Schubert, Adrian T. Press

ACS Nano· Volume 15· Issue 7
Publisher pageOpen full text
Source abstract

Abstract

Dye-loaded micelles of 10 nm diameter formed from amphiphilic graft copolymers composed of a hydrophobic poly(methyl methacrylate) backbone and hydrophilic poly(2-ethyl-2-oxazoline) side chains with a degree of polymerization of 15 were investigated concerning their cellular interaction and uptake in vitro as well as their interaction with local and circulating cells of the reticuloendothelial system in the liver by intravital microscopy. Despite the high molar mass of the individual macromolecules (Mn ≈ 20 kg mol-1), backbone end group modification by attachment of a hydrophilic anionic fluorescent probe strongly affected the in vivo performance. To understand these effects, the end group was additionally modified by the attachment of four methacrylic acid repeating units. Although various micelles appeared similar in dynamic light scattering and cryo-transmission electron microscopy, changes in the micelles were evident from principal component analysis of the Raman spectra. Whereas an efficient stealth effect was found for micelles formed from polymers with anionically charged or thiol end groups, a hydrophobic end group altered the micelles' structure sufficiently to adapt cell-type specificity and stealth properties in the liver.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

Polymers

OpenAlex topics: Nanoparticle-Based Drug Delivery · RNA Interference and Gene Delivery · Advanced Drug Delivery Systems

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743 Jena, Germany

Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany

Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany

Institute for Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Helmholtzweg 4, 07745 Jena, Germany

Leibniz Institute of Photonic Technology (IPHT) Jena, Member of the Leibniz Research Alliance - Leibniz Health Technologies, Albert-Einstein-Straße 9, 07745 Jena, Germany

Research Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute Jena, Adolf-Reichwein-Strasse 23, 07745 Jena, Germany

Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University Jena, Neugasse 24, 07743 Jena, Germany

Medical Faculty, Friedrich Schiller University Jena, Bachstraße 18, 07743 Jena, Germany

Funding

Th??ringer Aufbaubank · European Regional Development Fund · Deutsche Forschungsgemeinschaft

References (70)

Show source-provided references
1. 10.3762/bjnano.10.1610.3762/bjnano.10.16 ↗
2. 10.1124/pr.111.00536310.1124/pr.111.005363 ↗
3. 10.1038/nnano.2011.16610.1038/nnano.2011.166 ↗
4. 10.1016/0304-4165(81)90259-210.1016/0304-4165(81)90259-2 ↗
5. 10.1210/en.2009-108210.1210/en.2009-1082 ↗
6. 10.1073/pnas.88.24.1146010.1073/pnas.88.24.11460 ↗
7. 10.1016/0169-409X(95)00039-A10.1016/0169-409X(95)00039-A ↗
8. 10.1080/17425247.2016.118248510.1080/17425247.2016.1182485 ↗
9. 10.3109/0265204980900683110.3109/02652049809006831 ↗
10. 10.1016/S1359-6446(05)03575-010.1016/S1359-6446(05)03575-0 ↗
11. 10.1021/acs.analchem.6b0343710.1021/acs.analchem.6b03437 ↗
12. 10.1021/acs.analchem.6b0310910.1021/acs.analchem.6b03109 ↗
13. 10.1021/acsnano.9b0394210.1021/acsnano.9b03942 ↗
14. 10.1021/acsnano.9b0865910.1021/acsnano.9b08659 ↗
15. 10.1016/j.jconrel.2015.06.02310.1016/j.jconrel.2015.06.023 ↗
16. 10.1021/bc00030a00110.1021/bc00030a001 ↗
17. Moreadith R. W. · 2017 · 5110.1002/9781118967904.ch3 ↗
18. Danish, 100. Pipeline - Serina Therapeutics; https://serinatherapeutics.com/pipeline/ (accessed June 8, 2020).
19. 10.1002/adtp.20190016810.1002/adtp.201900168 ↗
20. 10.1002/marc.20120035410.1002/marc.201200354 ↗
21. 10.1021/acs.chemrev.9b0077410.1021/acs.chemrev.9b00774 ↗
22. 10.1002/mabi.20150018910.1002/mabi.201500189 ↗
23. 10.1002/jbm.a.3180010.1002/jbm.a.31800 ↗
24. 10.1002/adfm.20050011010.1002/adfm.200500110 ↗
25. 10.1016/j.polymer.2016.12.06810.1016/j.polymer.2016.12.068 ↗
26. 10.1039/c2cc30191a10.1039/c2cc30191a ↗
27. 10.1039/C6PY01130F10.1039/C6PY01130F ↗
28. 10.1002/pola.2853710.1002/pola.28537 ↗
29. 10.1021/acs.jpcb.5b1001910.1021/acs.jpcb.5b10019 ↗
30. 10.1039/C7SM00682A10.1039/C7SM00682A ↗
31. 10.1039/C9TB00118B10.1039/C9TB00118B ↗
32. 10.1021/acsmacrolett.9b0001610.1021/acsmacrolett.9b00016 ↗
33. 10.1016/S0168-3659(01)00451-510.1016/S0168-3659(01)00451-5 ↗
34. 10.1016/j.biomaterials.2011.01.02110.1016/j.biomaterials.2011.01.021 ↗
35. 10.1039/C6RA20368J10.1039/C6RA20368J ↗
36. 10.3390/ma1104052810.3390/ma11040528 ↗
37. 10.1038/am.2017.16110.1038/am.2017.161 ↗
38. 10.3389/fendo.2017.0026110.3389/fendo.2017.00261 ↗
39. 10.21769/BioProtoc.185910.21769/BioProtoc.1859 ↗
40. 10.1371/journal.pone.004579910.1371/journal.pone.0045799 ↗
41. 10.1038/srep0999410.1038/srep09994 ↗
42. 10.1038/nbt.261410.1038/nbt.2614 ↗
43. 10.1016/j.jconrel.2016.01.02010.1016/j.jconrel.2016.01.020 ↗
44. 10.1021/mp800051m10.1021/mp800051m ↗
45. 10.1021/acsnano.9b0138310.1021/acsnano.9b01383 ↗
46. 10.1016/0016-5085(81)90152-910.1016/0016-5085(81)90152-9 ↗
47. 10.1186/1743-8977-4-1010.1186/1743-8977-4-10 ↗
48. 10.1021/ja208433810.1021/ja2084338 ↗
49. 10.1016/j.jhep.2016.07.00910.1016/j.jhep.2016.07.009 ↗
50. 10.1152/ajpregu.00686.201110.1152/ajpregu.00686.2011 ↗
51. 10.1038/ncomms656510.1038/ncomms6565 ↗
52. 10.2147/IJN.S16880810.2147/IJN.S168808 ↗
53. 10.1021/acsnano.7b0699510.1021/acsnano.7b06995 ↗
54. 10.1021/nn303508c10.1021/nn303508c ↗
55. 10.1021/jp304630q10.1021/jp304630q ↗
56. 10.1016/j.jconrel.2015.08.00810.1016/j.jconrel.2015.08.008 ↗
57. 10.1021/acsnano.0c0235810.1021/acsnano.0c02358 ↗
58. 10.2217/nnm.15.18810.2217/nnm.15.188 ↗
59. 10.1021/ma902014q10.1021/ma902014q ↗
60. 10.1002/1097-0282(20001015)54:5<328::AID-BIP40>3.0.CO;2-P10.1002/1097-0282(20001015)54:5<328::AID-BIP40>3.0.CO;2-P ↗
61. 10.1021/acs.macromol.7b0266510.1021/acs.macromol.7b02665 ↗
62. 10.1021/acs.analchem.6b0361510.1021/acs.analchem.6b03615 ↗
63. 10.1016/j.ejpb.2013.01.02410.1016/j.ejpb.2013.01.024 ↗
64. 10.1021/bi00054a02110.1021/bi00054a021 ↗
65. 10.1016/S0005-2736(01)00389-310.1016/S0005-2736(01)00389-3 ↗
66. 10.1186/s12859-017-1934-z10.1186/s12859-017-1934-z ↗
67. 10.1038/nmeth.201910.1038/nmeth.2019 ↗
68. 10.1002/cyto.a.2320110.1002/cyto.a.23201 ↗
69. 10.1186/s12859-020-3363-710.1186/s12859-020-3363-7 ↗
70. 10.1109/TSMC.1979.431007610.1109/TSMC.1979.4310076 ↗