journal article·2020-06-23Open access

Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires

Yun X. Chen, Nicole L. Ing, Fengbin Wang, Dawei Xu, Nancy B. Sloan, Nga T. Lam, Daniel L. Winter, Edward H. Egelman, Allon I. Hochbaum, Douglas S. Clark, Dominic J. Glover

ACS Nano· Volume 14· Issue 6

Abstract

The transfer of electrons through protein complexes is central to cellular respiration. Exploiting proteins for charge transfer in a controllable fashion has the potential to revolutionize the integration of biological systems and electronic devices. Here we characterize the structure of an ultrastable protein filament and engineer the filament subunits to create electronically conductive nanowires under aqueous conditions. Cryoelectron microscopy was used to resolve the helical structure of gamma-prefoldin, a filamentous protein from a hyperthermophilic archaeon. Conjugation of tetra-heme c3-type cytochromes along the longitudinal axis of the filament created nanowires capable of long-range electron transfer. Electrochemical transport measurements indicated networks of the nanowires capable of conducting current between electrodes at the redox potential of the cytochromes. Functionalization of these highly engineerable nanowires with other molecules, such as redox enzymes, may be useful for bioelectronic applications.
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Research context

OpenAlex topics: Force Microscopy Techniques and Applications · Molecular Junctions and Nanostructures · Photosynthetic Processes and Mechanisms

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia

Department of Materials Science and Engineering, University of California, Irvine, California 92697, United States

Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, United States

Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States

Department of Chemistry, University of California, Irvine, California 92697, United States

Department of Chemical and Biomolecular Engineering, University of California, Irvine, California 92697, United States

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, United States

Funding

Air Force Office of Scientific Research · U.S. Department of Education · National Institute of General Medical Sciences

References (48)

Show source-provided references
1. 10.1038/nature0366110.1038/nature03661 ↗
2. 10.1038/nnano.2011.11910.1038/nnano.2011.119 ↗
3. 10.1073/pnas.120982910910.1073/pnas.1209829109 ↗
4. 10.1021/acs.jpcb.8b0743110.1021/acs.jpcb.8b07431 ↗
5. 10.1016/j.cell.2019.03.02910.1016/j.cell.2019.03.029 ↗
6. 10.1038/nchem.261610.1038/nchem.2616 ↗
7. 10.1021/acsnano.7b0875610.1021/acsnano.7b08756 ↗
8. 10.1021/acscentsci.6b0006710.1021/acscentsci.6b00067 ↗
9. 10.1038/ncomms1177110.1038/ncomms11771 ↗
10. 10.1110/ps.06259990710.1110/ps.062599907 ↗
11. 10.1007/978-3-030-00737-9_210.1007/978-3-030-00737-9_2 ↗
12. 10.1111/febs.1334110.1111/febs.13341 ↗
13. 10.1002/biot.20120000910.1002/biot.201200009 ↗
14. 10.1021/acssynbio.8b0024110.1021/acssynbio.8b00241 ↗
15. 10.1016/S0304-3991(00)00062-010.1016/S0304-3991(00)00062-0 ↗
16. 10.1016/j.jsb.2012.09.00610.1016/j.jsb.2012.09.006 ↗
17. 10.1016/j.jmb.2007.12.01010.1016/j.jmb.2007.12.010 ↗
18. 10.1016/j.sbi.2016.07.00910.1016/j.sbi.2016.07.009 ↗
19. 10.1107/S205979831800932410.1107/S2059798318009324 ↗
20. 10.1093/nar/gkw35710.1093/nar/gkw357 ↗
21. 10.1016/S0092-8674(00)00165-310.1016/S0092-8674(00)00165-3 ↗
22. 10.1016/j.jmb.2007.05.02210.1016/j.jmb.2007.05.022 ↗
23. 10.1073/pnas.94.10.538310.1073/pnas.94.10.5383 ↗
24. 10.1038/nrmicro.2017.15310.1038/nrmicro.2017.153 ↗
25. 10.1002/bip.2115710.1002/bip.21157 ↗
26. 10.1038/nature2300210.1038/nature23002 ↗
27. 10.1038/s41467-018-04290-w10.1038/s41467-018-04290-w ↗
28. 10.1128/AEM.66.9.4168-4171.200010.1128/AEM.66.9.4168-4171.2000 ↗
29. 10.1073/pnas.111548510910.1073/pnas.1115485109 ↗
30. 10.1039/C8CC08720B10.1039/C8CC08720B ↗
31. 10.1021/ja500215j10.1021/ja500215j ↗
32. 10.1016/0005-2728(75)90021-310.1016/0005-2728(75)90021-3 ↗
33. 10.1016/0022-2852(61)90236-310.1016/0022-2852(61)90236-3 ↗
34. 10.1039/C7CP03651E10.1039/C7CP03651E ↗
35. 10.1039/C5CP05152E10.1039/C5CP05152E ↗
36. 10.1038/nmeth.247210.1038/nmeth.2472 ↗
37. 10.1016/S1047-8477(03)00069-810.1016/S1047-8477(03)00069-8 ↗
38. 10.1016/j.jsb.2006.05.00910.1016/j.jsb.2006.05.009 ↗
39. 10.1002/jcc.2008410.1002/jcc.20084 ↗
40. 10.1038/nmeth.321310.1038/nmeth.3213 ↗
41. 10.1002/pro.327910.1002/pro.3279 ↗
42. 10.1002/pro.333010.1002/pro.3330 ↗
43. 10.1016/j.jmb.2013.10.02110.1016/j.jmb.2013.10.021 ↗
44. 10.1021/bi800570810.1021/bi8005708 ↗
45. 10.1038/nmeth.131810.1038/nmeth.1318 ↗
46. 10.1006/bbrc.1998.954910.1006/bbrc.1998.9549 ↗
47. 10.1006/abio.1996.017110.1006/abio.1996.0171 ↗
48. 10.1038/nmeth.208910.1038/nmeth.2089 ↗